SinR controls enterotoxin expression in Bacillus thuringiensis biofilms.

The entomopathogen Bacillus thuringiensis produces dense biofilms under various conditions. Here, we report that the transition phase regulators Spo0A, AbrB and SinR control biofilm formation and swimming motility in B. thuringiensis, just as they control biofilm formation and swarming motility in t...

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Autores principales:	Annette Fagerlund, Thomas Dubois, Ole-Andreas Økstad, Emilie Verplaetse, Nathalie Gilois, Imène Bennaceur, Stéphane Perchat, Myriam Gominet, Stéphane Aymerich, Anne-Brit Kolstø, Didier Lereclus, Michel Gohar
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/270b763accff40adbc2c3de15624b327
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spelling	oai:doaj.org-article:270b763accff40adbc2c3de15624b3272021-11-18T08:34:31ZSinR controls enterotoxin expression in Bacillus thuringiensis biofilms.1932-620310.1371/journal.pone.0087532https://doaj.org/article/270b763accff40adbc2c3de15624b3272014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24498128/?tool=EBIhttps://doaj.org/toc/1932-6203The entomopathogen Bacillus thuringiensis produces dense biofilms under various conditions. Here, we report that the transition phase regulators Spo0A, AbrB and SinR control biofilm formation and swimming motility in B. thuringiensis, just as they control biofilm formation and swarming motility in the closely related saprophyte species B. subtilis. However, microarray analysis indicated that in B. thuringiensis, in contrast to B. subtilis, SinR does not control an eps operon involved in exopolysaccharides production, but regulates genes involved in the biosynthesis of the lipopeptide kurstakin. This lipopeptide is required for biofilm formation and was previously shown to be important for survival in the host cadaver (necrotrophism). Microarray analysis also revealed that the SinR regulon contains genes coding for the Hbl enterotoxin. Transcriptional fusion assays, Western blots and hemolysis assays confirmed that SinR controls Hbl expression, together with PlcR, the main virulence regulator in B. thuringiensis. We show that Hbl is expressed in a sustained way in a small subpopulation of the biofilm, whereas almost all the planktonic population transiently expresses Hbl. The gene coding for SinI, an antagonist of SinR, is expressed in the same biofilm subpopulation as hbl, suggesting that hbl transcription heterogeneity is SinI-dependent. B. thuringiensis and B. cereus are enteric bacteria which possibly form biofilms lining the host intestinal epithelium. Toxins produced in biofilms could therefore be delivered directly to the target tissue.Annette FagerlundThomas DuboisOle-Andreas ØkstadEmilie VerplaetseNathalie GiloisImène BennaceurStéphane PerchatMyriam GominetStéphane AymerichAnne-Brit KolstøDidier LereclusMichel GoharPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e87532 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Annette Fagerlund Thomas Dubois Ole-Andreas Økstad Emilie Verplaetse Nathalie Gilois Imène Bennaceur Stéphane Perchat Myriam Gominet Stéphane Aymerich Anne-Brit Kolstø Didier Lereclus Michel Gohar SinR controls enterotoxin expression in Bacillus thuringiensis biofilms.
description	The entomopathogen Bacillus thuringiensis produces dense biofilms under various conditions. Here, we report that the transition phase regulators Spo0A, AbrB and SinR control biofilm formation and swimming motility in B. thuringiensis, just as they control biofilm formation and swarming motility in the closely related saprophyte species B. subtilis. However, microarray analysis indicated that in B. thuringiensis, in contrast to B. subtilis, SinR does not control an eps operon involved in exopolysaccharides production, but regulates genes involved in the biosynthesis of the lipopeptide kurstakin. This lipopeptide is required for biofilm formation and was previously shown to be important for survival in the host cadaver (necrotrophism). Microarray analysis also revealed that the SinR regulon contains genes coding for the Hbl enterotoxin. Transcriptional fusion assays, Western blots and hemolysis assays confirmed that SinR controls Hbl expression, together with PlcR, the main virulence regulator in B. thuringiensis. We show that Hbl is expressed in a sustained way in a small subpopulation of the biofilm, whereas almost all the planktonic population transiently expresses Hbl. The gene coding for SinI, an antagonist of SinR, is expressed in the same biofilm subpopulation as hbl, suggesting that hbl transcription heterogeneity is SinI-dependent. B. thuringiensis and B. cereus are enteric bacteria which possibly form biofilms lining the host intestinal epithelium. Toxins produced in biofilms could therefore be delivered directly to the target tissue.
format	article
author	Annette Fagerlund Thomas Dubois Ole-Andreas Økstad Emilie Verplaetse Nathalie Gilois Imène Bennaceur Stéphane Perchat Myriam Gominet Stéphane Aymerich Anne-Brit Kolstø Didier Lereclus Michel Gohar
author_facet	Annette Fagerlund Thomas Dubois Ole-Andreas Økstad Emilie Verplaetse Nathalie Gilois Imène Bennaceur Stéphane Perchat Myriam Gominet Stéphane Aymerich Anne-Brit Kolstø Didier Lereclus Michel Gohar
author_sort	Annette Fagerlund
title	SinR controls enterotoxin expression in Bacillus thuringiensis biofilms.
title_short	SinR controls enterotoxin expression in Bacillus thuringiensis biofilms.
title_full	SinR controls enterotoxin expression in Bacillus thuringiensis biofilms.
title_fullStr	SinR controls enterotoxin expression in Bacillus thuringiensis biofilms.
title_full_unstemmed	SinR controls enterotoxin expression in Bacillus thuringiensis biofilms.
title_sort	sinr controls enterotoxin expression in bacillus thuringiensis biofilms.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/270b763accff40adbc2c3de15624b327
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SinR controls enterotoxin expression in Bacillus thuringiensis biofilms.

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