Plasma-derived exosome characterization reveals a distinct microRNA signature in long duration Type 1 diabetes

Abstract Type 1 diabetes mellitus (T1DM) results from an autoimmune attack against the insulin-producing ß cells which leads to chronic hyperglycemia. Exosomes are lipid vesicles derived from cellular multivesicular bodies that are enriched in specific miRNAs, potentially providing a disease-specifi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Marta Garcia-Contreras, Sanket H. Shah, Alejandro Tamayo, Paul D. Robbins, Ronald B. Golberg, Armando J. Mendez, Camillo Ricordi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/270cea278d9d423e81a5e6af695e9c12
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:270cea278d9d423e81a5e6af695e9c12
record_format	dspace
spelling	oai:doaj.org-article:270cea278d9d423e81a5e6af695e9c122021-12-02T15:06:07ZPlasma-derived exosome characterization reveals a distinct microRNA signature in long duration Type 1 diabetes10.1038/s41598-017-05787-y2045-2322https://doaj.org/article/270cea278d9d423e81a5e6af695e9c122017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05787-yhttps://doaj.org/toc/2045-2322Abstract Type 1 diabetes mellitus (T1DM) results from an autoimmune attack against the insulin-producing ß cells which leads to chronic hyperglycemia. Exosomes are lipid vesicles derived from cellular multivesicular bodies that are enriched in specific miRNAs, potentially providing a disease-specific diagnostic signature. To assess the value of exosome miRNAs as biomarkers for T1DM, miRNA expression in plasma-derived exosomes was measured. Nanoparticle tracking analysis and transmission electron microscopy confirmed the presence of plasma-derived exosomes (EXOs) isolated by differential centrifugation. Total RNA extracted from plasma-derived EXOs of 12 T1DM and 12 control subjects was hybridized onto Nanostring human v2 miRNA microarray array and expression data were analyzed on nSolver analysis software. We found 7 different miRNAs (1 up-regulated and 6 down-regulated), that were differentially expressed in T1DM. The selected candidate miRNAs were validated by qRT-PCR analysis of cohorts of 24 T1DM and 24 control subjects. Most of the deregulated miRNAs are involved in progression of T1DM. These findings highlight the potential of EXOs miRNA profiling in the diagnosis as well as new insights into the molecular mechanisms involved in T1DM.Marta Garcia-ContrerasSanket H. ShahAlejandro TamayoPaul D. RobbinsRonald B. GolbergArmando J. MendezCamillo RicordiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marta Garcia-Contreras Sanket H. Shah Alejandro Tamayo Paul D. Robbins Ronald B. Golberg Armando J. Mendez Camillo Ricordi Plasma-derived exosome characterization reveals a distinct microRNA signature in long duration Type 1 diabetes
description	Abstract Type 1 diabetes mellitus (T1DM) results from an autoimmune attack against the insulin-producing ß cells which leads to chronic hyperglycemia. Exosomes are lipid vesicles derived from cellular multivesicular bodies that are enriched in specific miRNAs, potentially providing a disease-specific diagnostic signature. To assess the value of exosome miRNAs as biomarkers for T1DM, miRNA expression in plasma-derived exosomes was measured. Nanoparticle tracking analysis and transmission electron microscopy confirmed the presence of plasma-derived exosomes (EXOs) isolated by differential centrifugation. Total RNA extracted from plasma-derived EXOs of 12 T1DM and 12 control subjects was hybridized onto Nanostring human v2 miRNA microarray array and expression data were analyzed on nSolver analysis software. We found 7 different miRNAs (1 up-regulated and 6 down-regulated), that were differentially expressed in T1DM. The selected candidate miRNAs were validated by qRT-PCR analysis of cohorts of 24 T1DM and 24 control subjects. Most of the deregulated miRNAs are involved in progression of T1DM. These findings highlight the potential of EXOs miRNA profiling in the diagnosis as well as new insights into the molecular mechanisms involved in T1DM.
format	article
author	Marta Garcia-Contreras Sanket H. Shah Alejandro Tamayo Paul D. Robbins Ronald B. Golberg Armando J. Mendez Camillo Ricordi
author_facet	Marta Garcia-Contreras Sanket H. Shah Alejandro Tamayo Paul D. Robbins Ronald B. Golberg Armando J. Mendez Camillo Ricordi
author_sort	Marta Garcia-Contreras
title	Plasma-derived exosome characterization reveals a distinct microRNA signature in long duration Type 1 diabetes
title_short	Plasma-derived exosome characterization reveals a distinct microRNA signature in long duration Type 1 diabetes
title_full	Plasma-derived exosome characterization reveals a distinct microRNA signature in long duration Type 1 diabetes
title_fullStr	Plasma-derived exosome characterization reveals a distinct microRNA signature in long duration Type 1 diabetes
title_full_unstemmed	Plasma-derived exosome characterization reveals a distinct microRNA signature in long duration Type 1 diabetes
title_sort	plasma-derived exosome characterization reveals a distinct microrna signature in long duration type 1 diabetes
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/270cea278d9d423e81a5e6af695e9c12
work_keys_str_mv	AT martagarciacontreras plasmaderivedexosomecharacterizationrevealsadistinctmicrornasignatureinlongdurationtype1diabetes AT sankethshah plasmaderivedexosomecharacterizationrevealsadistinctmicrornasignatureinlongdurationtype1diabetes AT alejandrotamayo plasmaderivedexosomecharacterizationrevealsadistinctmicrornasignatureinlongdurationtype1diabetes AT pauldrobbins plasmaderivedexosomecharacterizationrevealsadistinctmicrornasignatureinlongdurationtype1diabetes AT ronaldbgolberg plasmaderivedexosomecharacterizationrevealsadistinctmicrornasignatureinlongdurationtype1diabetes AT armandojmendez plasmaderivedexosomecharacterizationrevealsadistinctmicrornasignatureinlongdurationtype1diabetes AT camilloricordi plasmaderivedexosomecharacterizationrevealsadistinctmicrornasignatureinlongdurationtype1diabetes
_version_	1718388566991568896

Plasma-derived exosome characterization reveals a distinct microRNA signature in long duration Type 1 diabetes

Ejemplares similares