Hypoperfusion of the infrapatellar fat pad and its relationship to MRI T2* relaxation time changes in a 5/6 nephrectomy model

Abstract The purpose of present study was to longitudinally investigate the alterations in infrapatellar fat pad (IPFP) vascularity in 5/6 nephrectomized rats by using dynamic contrast enhanced (DCE) MRI and IPFP degeneration by using MRI T2* relaxation time. Twelve male Sprague–Dawley rats were ass...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Guo-Shu Huang, Yi-Jen Peng, Yu-Juei Hsu, Herng-Sheng Lee, Yue-Cune Chang, Shih-Wei Chiang, Yi-Chih Hsu, Ying-Chun Liu, Ming-Huang Lin, Chao-Ying Wang
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
Materias:
R
Q
Acceso en línea:https://doaj.org/article/2713a5886b2542e5baaa1d712a24ecfd
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:2713a5886b2542e5baaa1d712a24ecfd
record_format dspace
spelling oai:doaj.org-article:2713a5886b2542e5baaa1d712a24ecfd2021-12-02T16:57:57ZHypoperfusion of the infrapatellar fat pad and its relationship to MRI T2* relaxation time changes in a 5/6 nephrectomy model10.1038/s41598-021-89336-82045-2322https://doaj.org/article/2713a5886b2542e5baaa1d712a24ecfd2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89336-8https://doaj.org/toc/2045-2322Abstract The purpose of present study was to longitudinally investigate the alterations in infrapatellar fat pad (IPFP) vascularity in 5/6 nephrectomized rats by using dynamic contrast enhanced (DCE) MRI and IPFP degeneration by using MRI T2* relaxation time. Twelve male Sprague–Dawley rats were assigned to a control group and a 5/6 nephrectomy CKD group. The right knees of all rats were longitudinally scanned by 4.7 T MRI, and serial changes in the IPFP were assessed at 0, 8, 16, 30, and 44 weeks by DCE-MRI (parameters A, k el and k ep ) and MRI T2* mapping. After MRI measurements, knee specimens were obtained and evaluated histologically. The CKD group had IPFPs with lower blood volume A and lower permeability k ep values from 16 weeks (p < 0.05), lower venous washout k el value from 30 weeks (p < 0.001), and significantly higher T2* values reflecting adipocyte degeneration beginning at 16 weeks (p < 0.05). The histopathological results confirmed the MRI findings. Hypoperfusion and adipocytes degeneration related to CKD were demonstrated in a rodent 5/6 nephrectomy model. DCE parameters and MRI T2* can serve as imaging biomarkers of fat pad degeneration during CKD progression.Guo-Shu HuangYi-Jen PengYu-Juei HsuHerng-Sheng LeeYue-Cune ChangShih-Wei ChiangYi-Chih HsuYing-Chun LiuMing-Huang LinChao-Ying WangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Guo-Shu Huang
Yi-Jen Peng
Yu-Juei Hsu
Herng-Sheng Lee
Yue-Cune Chang
Shih-Wei Chiang
Yi-Chih Hsu
Ying-Chun Liu
Ming-Huang Lin
Chao-Ying Wang
Hypoperfusion of the infrapatellar fat pad and its relationship to MRI T2* relaxation time changes in a 5/6 nephrectomy model
description Abstract The purpose of present study was to longitudinally investigate the alterations in infrapatellar fat pad (IPFP) vascularity in 5/6 nephrectomized rats by using dynamic contrast enhanced (DCE) MRI and IPFP degeneration by using MRI T2* relaxation time. Twelve male Sprague–Dawley rats were assigned to a control group and a 5/6 nephrectomy CKD group. The right knees of all rats were longitudinally scanned by 4.7 T MRI, and serial changes in the IPFP were assessed at 0, 8, 16, 30, and 44 weeks by DCE-MRI (parameters A, k el and k ep ) and MRI T2* mapping. After MRI measurements, knee specimens were obtained and evaluated histologically. The CKD group had IPFPs with lower blood volume A and lower permeability k ep values from 16 weeks (p < 0.05), lower venous washout k el value from 30 weeks (p < 0.001), and significantly higher T2* values reflecting adipocyte degeneration beginning at 16 weeks (p < 0.05). The histopathological results confirmed the MRI findings. Hypoperfusion and adipocytes degeneration related to CKD were demonstrated in a rodent 5/6 nephrectomy model. DCE parameters and MRI T2* can serve as imaging biomarkers of fat pad degeneration during CKD progression.
format article
author Guo-Shu Huang
Yi-Jen Peng
Yu-Juei Hsu
Herng-Sheng Lee
Yue-Cune Chang
Shih-Wei Chiang
Yi-Chih Hsu
Ying-Chun Liu
Ming-Huang Lin
Chao-Ying Wang
author_facet Guo-Shu Huang
Yi-Jen Peng
Yu-Juei Hsu
Herng-Sheng Lee
Yue-Cune Chang
Shih-Wei Chiang
Yi-Chih Hsu
Ying-Chun Liu
Ming-Huang Lin
Chao-Ying Wang
author_sort Guo-Shu Huang
title Hypoperfusion of the infrapatellar fat pad and its relationship to MRI T2* relaxation time changes in a 5/6 nephrectomy model
title_short Hypoperfusion of the infrapatellar fat pad and its relationship to MRI T2* relaxation time changes in a 5/6 nephrectomy model
title_full Hypoperfusion of the infrapatellar fat pad and its relationship to MRI T2* relaxation time changes in a 5/6 nephrectomy model
title_fullStr Hypoperfusion of the infrapatellar fat pad and its relationship to MRI T2* relaxation time changes in a 5/6 nephrectomy model
title_full_unstemmed Hypoperfusion of the infrapatellar fat pad and its relationship to MRI T2* relaxation time changes in a 5/6 nephrectomy model
title_sort hypoperfusion of the infrapatellar fat pad and its relationship to mri t2* relaxation time changes in a 5/6 nephrectomy model
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/2713a5886b2542e5baaa1d712a24ecfd
work_keys_str_mv AT guoshuhuang hypoperfusionoftheinfrapatellarfatpadanditsrelationshiptomrit2relaxationtimechangesina56nephrectomymodel
AT yijenpeng hypoperfusionoftheinfrapatellarfatpadanditsrelationshiptomrit2relaxationtimechangesina56nephrectomymodel
AT yujueihsu hypoperfusionoftheinfrapatellarfatpadanditsrelationshiptomrit2relaxationtimechangesina56nephrectomymodel
AT herngshenglee hypoperfusionoftheinfrapatellarfatpadanditsrelationshiptomrit2relaxationtimechangesina56nephrectomymodel
AT yuecunechang hypoperfusionoftheinfrapatellarfatpadanditsrelationshiptomrit2relaxationtimechangesina56nephrectomymodel
AT shihweichiang hypoperfusionoftheinfrapatellarfatpadanditsrelationshiptomrit2relaxationtimechangesina56nephrectomymodel
AT yichihhsu hypoperfusionoftheinfrapatellarfatpadanditsrelationshiptomrit2relaxationtimechangesina56nephrectomymodel
AT yingchunliu hypoperfusionoftheinfrapatellarfatpadanditsrelationshiptomrit2relaxationtimechangesina56nephrectomymodel
AT minghuanglin hypoperfusionoftheinfrapatellarfatpadanditsrelationshiptomrit2relaxationtimechangesina56nephrectomymodel
AT chaoyingwang hypoperfusionoftheinfrapatellarfatpadanditsrelationshiptomrit2relaxationtimechangesina56nephrectomymodel
_version_ 1718382436583211008