Nanomedicine for glaucoma: liposomes provide sustained release of latanoprost in the eye

Jayaganesh V Natarajan1*, Marcus Ang2*, Anastasia Darwitan1, Sujay Chattopadhyay3, Tina T Wong2, Subbu S Venkatraman1 1Materials Science and Engineering, Nanyang Technological University, Singapore; 2Singapore Eye Research Institute, Singapore; 3Polymer Division, Indian Institute of Technology Roork...

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Autores principales:	Natarajan JV, Ang M, Darwitan A, Chattopadhyay S, Wong TT, Venkatraman SS
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/2713e1fa08a1431d989499c9486fde02
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spelling	oai:doaj.org-article:2713e1fa08a1431d989499c9486fde022021-12-02T05:09:45ZNanomedicine for glaucoma: liposomes provide sustained release of latanoprost in the eye1176-91141178-2013https://doaj.org/article/2713e1fa08a1431d989499c9486fde022012-01-01T00:00:00Zhttp://www.dovepress.com/nanomedicine-for-glaucoma-liposomes-provide-sustained-release-of-latan-a9000https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Jayaganesh V Natarajan1, Marcus Ang2, Anastasia Darwitan1, Sujay Chattopadhyay3, Tina T Wong2, Subbu S Venkatraman1 1Materials Science and Engineering, Nanyang Technological University, Singapore; 2Singapore Eye Research Institute, Singapore; 3Polymer Division, Indian Institute of Technology Roorkee, India*These authors contributed equally to this workPurpose: To report the development and therapeutic evaluation of a liposomal nanocarrier for sustained release of latanoprost, in the rabbit eye.Methods: We fabricated latanoprost-loaded egg-phosphatidylcholine (EggPC) liposomes using the film hydration technique. The delivery vehicles were nano-sized (Z avg = 109 &plusmn; 18 nm), had a narrow poly dispersity index (PDI = 0.19 &plusmn; 0.04), and a very high loading efficiency (94% &plusmn; 5%). Based on in vitro data, we evaluated this formulation for lowering intraocular pressure (IOP) in rabbit eyes. Following a single subconjunctival injection of the latanoprost loaded formulation, the eyes were clinically monitored and the IOP recorded.Results: Latanoprost-loaded EggPC liposomes demonstrated a high drug/lipid mole ratio of 0.181, remained stable for at least 6 months on storage (4&deg;C), and at least 1 month at 25&deg;C. A slow and sustained release of 60% of latanoprost was achieved by 14 days in the in vitro release study. The same formulation demonstrated a greater sustained IOP lowering effect compared with daily administration of topical latanoprost beyond 90 days (4.8 &plusmn; 1.5 vs 2.5 &plusmn; 0.9 mmHg; P &lt; 0.001). No signs of inflammation were evident in the eyes from slit-lamp examination analysis.Conclusion: The loading required for a long-term sustained delivery of latanoprost for up to 90 days in the rabbit eyes was achieved with EggPC liposomes. A single injection of latanoprost-loaded EggPC liposomes can lower the IOP for up to 90 days, with a greater IOP lowering effect than daily topical administration of latanoprost.Keywords: nanomedicine, nanoliposomes, EggPC, latanoprost, sustained ocular delivery, glaucomaNatarajan JVAng MDarwitan AChattopadhyay SWong TTVenkatraman SSDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 123-131 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Natarajan JV Ang M Darwitan A Chattopadhyay S Wong TT Venkatraman SS Nanomedicine for glaucoma: liposomes provide sustained release of latanoprost in the eye
description	Jayaganesh V Natarajan1, Marcus Ang2, Anastasia Darwitan1, Sujay Chattopadhyay3, Tina T Wong2, Subbu S Venkatraman1 1Materials Science and Engineering, Nanyang Technological University, Singapore; 2Singapore Eye Research Institute, Singapore; 3Polymer Division, Indian Institute of Technology Roorkee, India*These authors contributed equally to this workPurpose: To report the development and therapeutic evaluation of a liposomal nanocarrier for sustained release of latanoprost, in the rabbit eye.Methods: We fabricated latanoprost-loaded egg-phosphatidylcholine (EggPC) liposomes using the film hydration technique. The delivery vehicles were nano-sized (Z avg = 109 &plusmn; 18 nm), had a narrow poly dispersity index (PDI = 0.19 &plusmn; 0.04), and a very high loading efficiency (94% &plusmn; 5%). Based on in vitro data, we evaluated this formulation for lowering intraocular pressure (IOP) in rabbit eyes. Following a single subconjunctival injection of the latanoprost loaded formulation, the eyes were clinically monitored and the IOP recorded.Results: Latanoprost-loaded EggPC liposomes demonstrated a high drug/lipid mole ratio of 0.181, remained stable for at least 6 months on storage (4&deg;C), and at least 1 month at 25&deg;C. A slow and sustained release of 60% of latanoprost was achieved by 14 days in the in vitro release study. The same formulation demonstrated a greater sustained IOP lowering effect compared with daily administration of topical latanoprost beyond 90 days (4.8 &plusmn; 1.5 vs 2.5 &plusmn; 0.9 mmHg; P &lt; 0.001). No signs of inflammation were evident in the eyes from slit-lamp examination analysis.Conclusion: The loading required for a long-term sustained delivery of latanoprost for up to 90 days in the rabbit eyes was achieved with EggPC liposomes. A single injection of latanoprost-loaded EggPC liposomes can lower the IOP for up to 90 days, with a greater IOP lowering effect than daily topical administration of latanoprost.Keywords: nanomedicine, nanoliposomes, EggPC, latanoprost, sustained ocular delivery, glaucoma
format	article
author	Natarajan JV Ang M Darwitan A Chattopadhyay S Wong TT Venkatraman SS
author_facet	Natarajan JV Ang M Darwitan A Chattopadhyay S Wong TT Venkatraman SS
author_sort	Natarajan JV
title	Nanomedicine for glaucoma: liposomes provide sustained release of latanoprost in the eye
title_short	Nanomedicine for glaucoma: liposomes provide sustained release of latanoprost in the eye
title_full	Nanomedicine for glaucoma: liposomes provide sustained release of latanoprost in the eye
title_fullStr	Nanomedicine for glaucoma: liposomes provide sustained release of latanoprost in the eye
title_full_unstemmed	Nanomedicine for glaucoma: liposomes provide sustained release of latanoprost in the eye
title_sort	nanomedicine for glaucoma: liposomes provide sustained release of latanoprost in the eye
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/2713e1fa08a1431d989499c9486fde02
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Nanomedicine for glaucoma: liposomes provide sustained release of latanoprost in the eye

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