Sputum inflammatory profile before and after specific inhalation challenge in individuals with suspected occupational asthma.

<h4>Background</h4>The aim of this study was to establish the sputum inflammatory profile and changes in levels of leukotriene B₄ (LTB₄) and a panel of Th1/Th2 cytokines in subjects with suspected occupational asthma (OA) following specific inhalation challenge (SIC) to high-molecular-we...

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Autores principales:	Sara Sánchez-Vidaurre, María-Jesús Cruz, Susana Gómez-Ollés, Ferran Morell, Xavier Muñoz
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2716445f9f13414d8d67b52f3b998f80
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spelling	oai:doaj.org-article:2716445f9f13414d8d67b52f3b998f802021-11-18T08:46:57ZSputum inflammatory profile before and after specific inhalation challenge in individuals with suspected occupational asthma.1932-620310.1371/journal.pone.0078304https://doaj.org/article/2716445f9f13414d8d67b52f3b998f802013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24236015/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The aim of this study was to establish the sputum inflammatory profile and changes in levels of leukotriene B₄ (LTB₄) and a panel of Th1/Th2 cytokines in subjects with suspected occupational asthma (OA) following specific inhalation challenge (SIC) to high-molecular-weight (HMW) and low-molecular-weight (LMW) agents.<h4>Material and methods</h4>Fifty-one consecutive subjects undergoing SIC for suspected OA were enrolled. Sputum induction was performed the day before and 24 h after exposure to the offending agent. Total and differential cell counts were assessed. LTB₄ and a 10 Th1/Th2 cytokines were measured in sputum supernatant.<h4>Results</h4>Thirty-four patients tested positive to SIC and were diagnosed with OA (in 10 due to HMW agents and in 24 to LMW agents). SIC was negative in 17 subjects. As compared to baseline an increase was found in the percentage of sputum eosinophils and neutrophils, and in IL-10 concentration after SIC (p = 0.0078, p = 0.0195, and p = 0.046, respectively), and a decrease was seen in LTB₄ level (p = 0.0078) in patients with OA due to HMW agents. An increase in the percentage of sputum neutrophils after SIC (p = 0.0040) was observed in subjects without OA exposed to LMW agents. IL-8 levels after SIC were higher in patients without OA compared with patients with OA (p = 0.0146).<h4>Conclusion</h4>When conducting airway inflammation studies in OA, patients should be divided according to the causal agent (HMW or LMW). In OA patients exposed to HMW agents, an increase in the number of neutrophils can be found in parallel to the increase of eosinophils, although this does not contradict an IgE-mediated mechanism. Exposure to LMW agents can result in increased neutrophilic inflammation in patients with airway diseases unrelated to OA. There is variability in the responses observed in patients with OA exposed to LMW agents.Sara Sánchez-VidaurreMaría-Jesús CruzSusana Gómez-OllésFerran MorellXavier MuñozPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 11, p e78304 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sara Sánchez-Vidaurre María-Jesús Cruz Susana Gómez-Ollés Ferran Morell Xavier Muñoz Sputum inflammatory profile before and after specific inhalation challenge in individuals with suspected occupational asthma.
description	<h4>Background</h4>The aim of this study was to establish the sputum inflammatory profile and changes in levels of leukotriene B₄ (LTB₄) and a panel of Th1/Th2 cytokines in subjects with suspected occupational asthma (OA) following specific inhalation challenge (SIC) to high-molecular-weight (HMW) and low-molecular-weight (LMW) agents.<h4>Material and methods</h4>Fifty-one consecutive subjects undergoing SIC for suspected OA were enrolled. Sputum induction was performed the day before and 24 h after exposure to the offending agent. Total and differential cell counts were assessed. LTB₄ and a 10 Th1/Th2 cytokines were measured in sputum supernatant.<h4>Results</h4>Thirty-four patients tested positive to SIC and were diagnosed with OA (in 10 due to HMW agents and in 24 to LMW agents). SIC was negative in 17 subjects. As compared to baseline an increase was found in the percentage of sputum eosinophils and neutrophils, and in IL-10 concentration after SIC (p = 0.0078, p = 0.0195, and p = 0.046, respectively), and a decrease was seen in LTB₄ level (p = 0.0078) in patients with OA due to HMW agents. An increase in the percentage of sputum neutrophils after SIC (p = 0.0040) was observed in subjects without OA exposed to LMW agents. IL-8 levels after SIC were higher in patients without OA compared with patients with OA (p = 0.0146).<h4>Conclusion</h4>When conducting airway inflammation studies in OA, patients should be divided according to the causal agent (HMW or LMW). In OA patients exposed to HMW agents, an increase in the number of neutrophils can be found in parallel to the increase of eosinophils, although this does not contradict an IgE-mediated mechanism. Exposure to LMW agents can result in increased neutrophilic inflammation in patients with airway diseases unrelated to OA. There is variability in the responses observed in patients with OA exposed to LMW agents.
format	article
author	Sara Sánchez-Vidaurre María-Jesús Cruz Susana Gómez-Ollés Ferran Morell Xavier Muñoz
author_facet	Sara Sánchez-Vidaurre María-Jesús Cruz Susana Gómez-Ollés Ferran Morell Xavier Muñoz
author_sort	Sara Sánchez-Vidaurre
title	Sputum inflammatory profile before and after specific inhalation challenge in individuals with suspected occupational asthma.
title_short	Sputum inflammatory profile before and after specific inhalation challenge in individuals with suspected occupational asthma.
title_full	Sputum inflammatory profile before and after specific inhalation challenge in individuals with suspected occupational asthma.
title_fullStr	Sputum inflammatory profile before and after specific inhalation challenge in individuals with suspected occupational asthma.
title_full_unstemmed	Sputum inflammatory profile before and after specific inhalation challenge in individuals with suspected occupational asthma.
title_sort	sputum inflammatory profile before and after specific inhalation challenge in individuals with suspected occupational asthma.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/2716445f9f13414d8d67b52f3b998f80
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Sputum inflammatory profile before and after specific inhalation challenge in individuals with suspected occupational asthma.

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