Potential biomarkers of childhood brain tumor identified by proteomics of cerebrospinal fluid from extraventricular drainage (EVD)

Potential biomarkers of childhood brain tumor identified by proteomics of cerebrospinal fluid from extraventricular drainage (EVD)

Abstract Brain tumors are the most common solid tumors in childhood. There is the need for biomarkers of residual disease, therapy response and recurrence. Cerebrospinal fluid (CSF) is a source of brain tumor biomarkers. We analyzed the proteome of waste CSF from extraventricular drainage (EVD) from...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Maurizio Bruschi, Andrea Petretto, Armando Cama, Marco Pavanello, Martina Bartolucci, Giovanni Morana, Luca Antonio Ramenghi, Maria Luisa Garré, Gian Marco Ghiggeri, Isabella Panfoli, Giovanni Candiano
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
Materias:
Medicine
R
Science
Q
Acceso en línea:https://doaj.org/article/27191e19a4334a8383599b8230036639
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:27191e19a4334a8383599b8230036639
record_format dspace
spelling oai:doaj.org-article:27191e19a4334a8383599b82300366392021-12-02T13:50:49ZPotential biomarkers of childhood brain tumor identified by proteomics of cerebrospinal fluid from extraventricular drainage (EVD)10.1038/s41598-020-80647-w2045-2322https://doaj.org/article/27191e19a4334a8383599b82300366392021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80647-whttps://doaj.org/toc/2045-2322Abstract Brain tumors are the most common solid tumors in childhood. There is the need for biomarkers of residual disease, therapy response and recurrence. Cerebrospinal fluid (CSF) is a source of brain tumor biomarkers. We analyzed the proteome of waste CSF from extraventricular drainage (EVD) from 29 children bearing different brain tumors and 17 controls needing EVD insertion for unrelated causes. 1598 and 1526 proteins were identified by liquid chromatography-coupled tandem mass spectrometry proteomics in CSF control and brain tumor patients, respectively, 263 and 191 proteins being exclusive of either condition. Bioinformatic analysis revealed promising protein biomarkers for the discrimination between control and tumor (TATA-binding protein-associated factor 15 and S100 protein B). Moreover, Thymosin beta-4 (TMSB4X) and CD109, and 14.3.3 and HSP90 alpha could discriminate among other brain tumors and low-grade gliomas plus glyoneuronal tumors/pilocytic astrocytoma, or embryonal tumors/medulloblastoma. Biomarkers were validated by ELISA assay. Our method was able to distinguish among brain tumor vs non-tumor/hemorrhagic conditions (controls) and to differentiate two large classes of brain tumors. Further prospective studies may assess whether the biomarkers proposed by our discovery approach can be identified in other bodily fluids, therefore less invasively, and are useful to guide therapy and predict recurrences.Maurizio BruschiAndrea PetrettoArmando CamaMarco PavanelloMartina BartolucciGiovanni MoranaLuca Antonio RamenghiMaria Luisa GarréGian Marco GhiggeriIsabella PanfoliGiovanni CandianoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Maurizio Bruschi
Andrea Petretto
Armando Cama
Marco Pavanello
Martina Bartolucci
Giovanni Morana
Luca Antonio Ramenghi
Maria Luisa Garré
Gian Marco Ghiggeri
Isabella Panfoli
Giovanni Candiano
Potential biomarkers of childhood brain tumor identified by proteomics of cerebrospinal fluid from extraventricular drainage (EVD)
description Abstract Brain tumors are the most common solid tumors in childhood. There is the need for biomarkers of residual disease, therapy response and recurrence. Cerebrospinal fluid (CSF) is a source of brain tumor biomarkers. We analyzed the proteome of waste CSF from extraventricular drainage (EVD) from 29 children bearing different brain tumors and 17 controls needing EVD insertion for unrelated causes. 1598 and 1526 proteins were identified by liquid chromatography-coupled tandem mass spectrometry proteomics in CSF control and brain tumor patients, respectively, 263 and 191 proteins being exclusive of either condition. Bioinformatic analysis revealed promising protein biomarkers for the discrimination between control and tumor (TATA-binding protein-associated factor 15 and S100 protein B). Moreover, Thymosin beta-4 (TMSB4X) and CD109, and 14.3.3 and HSP90 alpha could discriminate among other brain tumors and low-grade gliomas plus glyoneuronal tumors/pilocytic astrocytoma, or embryonal tumors/medulloblastoma. Biomarkers were validated by ELISA assay. Our method was able to distinguish among brain tumor vs non-tumor/hemorrhagic conditions (controls) and to differentiate two large classes of brain tumors. Further prospective studies may assess whether the biomarkers proposed by our discovery approach can be identified in other bodily fluids, therefore less invasively, and are useful to guide therapy and predict recurrences.
format article
author Maurizio Bruschi
Andrea Petretto
Armando Cama
Marco Pavanello
Martina Bartolucci
Giovanni Morana
Luca Antonio Ramenghi
Maria Luisa Garré
Gian Marco Ghiggeri
Isabella Panfoli
Giovanni Candiano
author_facet Maurizio Bruschi
Andrea Petretto
Armando Cama
Marco Pavanello
Martina Bartolucci
Giovanni Morana
Luca Antonio Ramenghi
Maria Luisa Garré
Gian Marco Ghiggeri
Isabella Panfoli
Giovanni Candiano
author_sort Maurizio Bruschi
title Potential biomarkers of childhood brain tumor identified by proteomics of cerebrospinal fluid from extraventricular drainage (EVD)
title_short Potential biomarkers of childhood brain tumor identified by proteomics of cerebrospinal fluid from extraventricular drainage (EVD)
title_full Potential biomarkers of childhood brain tumor identified by proteomics of cerebrospinal fluid from extraventricular drainage (EVD)
title_fullStr Potential biomarkers of childhood brain tumor identified by proteomics of cerebrospinal fluid from extraventricular drainage (EVD)
title_full_unstemmed Potential biomarkers of childhood brain tumor identified by proteomics of cerebrospinal fluid from extraventricular drainage (EVD)
title_sort potential biomarkers of childhood brain tumor identified by proteomics of cerebrospinal fluid from extraventricular drainage (evd)
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/27191e19a4334a8383599b8230036639
work_keys_str_mv AT mauriziobruschi potentialbiomarkersofchildhoodbraintumoridentifiedbyproteomicsofcerebrospinalfluidfromextraventriculardrainageevd
AT andreapetretto potentialbiomarkersofchildhoodbraintumoridentifiedbyproteomicsofcerebrospinalfluidfromextraventriculardrainageevd
AT armandocama potentialbiomarkersofchildhoodbraintumoridentifiedbyproteomicsofcerebrospinalfluidfromextraventriculardrainageevd
AT marcopavanello potentialbiomarkersofchildhoodbraintumoridentifiedbyproteomicsofcerebrospinalfluidfromextraventriculardrainageevd
AT martinabartolucci potentialbiomarkersofchildhoodbraintumoridentifiedbyproteomicsofcerebrospinalfluidfromextraventriculardrainageevd
AT giovannimorana potentialbiomarkersofchildhoodbraintumoridentifiedbyproteomicsofcerebrospinalfluidfromextraventriculardrainageevd
AT lucaantonioramenghi potentialbiomarkersofchildhoodbraintumoridentifiedbyproteomicsofcerebrospinalfluidfromextraventriculardrainageevd
AT marialuisagarre potentialbiomarkersofchildhoodbraintumoridentifiedbyproteomicsofcerebrospinalfluidfromextraventriculardrainageevd
AT gianmarcoghiggeri potentialbiomarkersofchildhoodbraintumoridentifiedbyproteomicsofcerebrospinalfluidfromextraventriculardrainageevd
AT isabellapanfoli potentialbiomarkersofchildhoodbraintumoridentifiedbyproteomicsofcerebrospinalfluidfromextraventriculardrainageevd
AT giovannicandiano potentialbiomarkersofchildhoodbraintumoridentifiedbyproteomicsofcerebrospinalfluidfromextraventriculardrainageevd
_version_ 1718392427895586816

Ejemplares similares