Lipase-producing ability of bacteria from inasua (fish fermented product) from Central Moluccas, Indonesia

Abstract. Anggriani L, Budiarti S, Mubarik NR. 2020. Lipase-producing ability of bacteria from inasua (fish fermented product) from Central Moluccas, Indonesia. Biodiversitas 21: 622-628. One of fish preservation techniques through fermentation is Inasua which occurs spontaneously and involves vario...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Latusi Anggriani, SRI BUDIARTI, NISA RACHMANIA MUBARIK
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	16s rrna gene, bacillus, fish fermented, lipase-producing Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/271e10d9cac346a484824c3c6024a493
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:271e10d9cac346a484824c3c6024a493
record_format	dspace
spelling	oai:doaj.org-article:271e10d9cac346a484824c3c6024a4932021-11-21T22:01:10ZLipase-producing ability of bacteria from inasua (fish fermented product) from Central Moluccas, Indonesia1412-033X2085-472210.13057/biodiv/d210226https://doaj.org/article/271e10d9cac346a484824c3c6024a4932020-01-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4488https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Anggriani L, Budiarti S, Mubarik NR. 2020. Lipase-producing ability of bacteria from inasua (fish fermented product) from Central Moluccas, Indonesia. Biodiversitas 21: 622-628. One of fish preservation techniques through fermentation is Inasua which occurs spontaneously and involves various types of microorganisms. Isolates from Inasua are believed to have the ability to produce lipolytic enzymes. The purpose of this study was to identify potential bacterial isolates isolated from Inasua and to determine their lipase producing ability. The confirmation test of lipase-producing ability was conducted by inoculating the microbes into media containing Nutrient agar (NA), olive oil, and rhodamin-B. Lipolytic bacteria were detected using a screening method with UV light. From the results, there were 5 isolates of bacteria that showed lipolytic activity as IG 3.1, IG 6, IG 10, IG 12, IG 66. Lipase assay was determined using spectrophotometric method with p-nitrophenyl palmitate (pNPP) as a substrate and titration method with olive oil as a substrate. The enzyme showed maximum activity at pNPP as a substrate. IG 6 and IG 12 gave the highest activity from lipase assay. Growth and production curves were created before partial purification was carried out. The results of the curves showed that the optimum activity of IG 6 and IG 12 were in the stationary phase with value 38.16 U.mg-1 protein and 7.01 U.mg-1 protein, respectively. After ammonium sulfate precipitation, the activity of the lipase IG 6 and IG 12 were found to be 39.65 U.mL-1 and 37.05 U.mL-1, respectively. During optimization, the enzyme showed maximum activity at pH 8/70°C temperature. Molecular identification by using the 16S rRNA gene and phylogenetic analysis showed that isolates IG 6 and IG12 are closely related to Bacillus paramycoides and Bacillus kochii, respectively.Latusi AnggrianiSRI BUDIARTINISA RACHMANIA MUBARIKMBI & UNS Soloarticle16s rrna gene, bacillus, fish fermented, lipase-producingBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 2 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	16s rrna gene, bacillus, fish fermented, lipase-producing Biology (General) QH301-705.5
spellingShingle	16s rrna gene, bacillus, fish fermented, lipase-producing Biology (General) QH301-705.5 Latusi Anggriani SRI BUDIARTI NISA RACHMANIA MUBARIK Lipase-producing ability of bacteria from inasua (fish fermented product) from Central Moluccas, Indonesia
description	Abstract. Anggriani L, Budiarti S, Mubarik NR. 2020. Lipase-producing ability of bacteria from inasua (fish fermented product) from Central Moluccas, Indonesia. Biodiversitas 21: 622-628. One of fish preservation techniques through fermentation is Inasua which occurs spontaneously and involves various types of microorganisms. Isolates from Inasua are believed to have the ability to produce lipolytic enzymes. The purpose of this study was to identify potential bacterial isolates isolated from Inasua and to determine their lipase producing ability. The confirmation test of lipase-producing ability was conducted by inoculating the microbes into media containing Nutrient agar (NA), olive oil, and rhodamin-B. Lipolytic bacteria were detected using a screening method with UV light. From the results, there were 5 isolates of bacteria that showed lipolytic activity as IG 3.1, IG 6, IG 10, IG 12, IG 66. Lipase assay was determined using spectrophotometric method with p-nitrophenyl palmitate (pNPP) as a substrate and titration method with olive oil as a substrate. The enzyme showed maximum activity at pNPP as a substrate. IG 6 and IG 12 gave the highest activity from lipase assay. Growth and production curves were created before partial purification was carried out. The results of the curves showed that the optimum activity of IG 6 and IG 12 were in the stationary phase with value 38.16 U.mg-1 protein and 7.01 U.mg-1 protein, respectively. After ammonium sulfate precipitation, the activity of the lipase IG 6 and IG 12 were found to be 39.65 U.mL-1 and 37.05 U.mL-1, respectively. During optimization, the enzyme showed maximum activity at pH 8/70°C temperature. Molecular identification by using the 16S rRNA gene and phylogenetic analysis showed that isolates IG 6 and IG12 are closely related to Bacillus paramycoides and Bacillus kochii, respectively.
format	article
author	Latusi Anggriani SRI BUDIARTI NISA RACHMANIA MUBARIK
author_facet	Latusi Anggriani SRI BUDIARTI NISA RACHMANIA MUBARIK
author_sort	Latusi Anggriani
title	Lipase-producing ability of bacteria from inasua (fish fermented product) from Central Moluccas, Indonesia
title_short	Lipase-producing ability of bacteria from inasua (fish fermented product) from Central Moluccas, Indonesia
title_full	Lipase-producing ability of bacteria from inasua (fish fermented product) from Central Moluccas, Indonesia
title_fullStr	Lipase-producing ability of bacteria from inasua (fish fermented product) from Central Moluccas, Indonesia
title_full_unstemmed	Lipase-producing ability of bacteria from inasua (fish fermented product) from Central Moluccas, Indonesia
title_sort	lipase-producing ability of bacteria from inasua (fish fermented product) from central moluccas, indonesia
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/271e10d9cac346a484824c3c6024a493
work_keys_str_mv	AT latusianggriani lipaseproducingabilityofbacteriafrominasuafishfermentedproductfromcentralmoluccasindonesia AT sribudiarti lipaseproducingabilityofbacteriafrominasuafishfermentedproductfromcentralmoluccasindonesia AT nisarachmaniamubarik lipaseproducingabilityofbacteriafrominasuafishfermentedproductfromcentralmoluccasindonesia
_version_	1718418571979128832

Lipase-producing ability of bacteria from inasua (fish fermented product) from Central Moluccas, Indonesia

Ejemplares similares