Characterization of chemically induced ovarian carcinomas in an ethanol-preferring rat model: influence of long-term melatonin treatment.

Ovarian cancer is the fourth most common cause of cancer deaths among women, and chronic alcoholism may exert co-carcinogenic effects. Because melatonin (mel) has oncostatic properties, we aimed to investigate and characterize the chemical induction of ovarian tumors in a model of ethanol-preferring...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Luiz Gustavo A Chuffa, Beatriz A Fioruci-Fontanelli, Leonardo O Mendes, Wagner J Fávaro, Patricia Fernanda F Pinheiro, Marcelo Martinez, Francisco Eduardo Martinez
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/27280d8f53a74b8eb1daf6b55bd82ae7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:27280d8f53a74b8eb1daf6b55bd82ae7
record_format	dspace
spelling	oai:doaj.org-article:27280d8f53a74b8eb1daf6b55bd82ae72021-11-18T08:41:26ZCharacterization of chemically induced ovarian carcinomas in an ethanol-preferring rat model: influence of long-term melatonin treatment.1932-620310.1371/journal.pone.0081676https://doaj.org/article/27280d8f53a74b8eb1daf6b55bd82ae72013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24367487/?tool=EBIhttps://doaj.org/toc/1932-6203Ovarian cancer is the fourth most common cause of cancer deaths among women, and chronic alcoholism may exert co-carcinogenic effects. Because melatonin (mel) has oncostatic properties, we aimed to investigate and characterize the chemical induction of ovarian tumors in a model of ethanol-preferring rats and to verify the influence of mel treatment on the overall features of these tumors. After rats were selected to receive ethanol (EtOH), they were surgically injected with 100 µg of 7,12-dimethyl-benz[a]anthracene (DMBA) plus sesame oil directly under the left ovarian bursa. At 260 days old, half of the animals received i.p. injections of 200 µg mel/100 g b.w. for 60 days. Four experimental groups were established: Group C, rats bearing ovarian carcinomas (OC); Group C+EtOH, rats voluntarily consuming 10% (v/v) EtOH and bearing OC; Group C+M, rats bearing OC and receiving mel; and Group C+EtOH+M, rats with OC consuming EtOH and receiving mel. Estrous cycle and nutritional parameters were evaluated, and anatomopathological analyses of the ovarian tumors were conducted. The incidence of ovarian tumors was higher in EtOH drinking animals 120 days post-DMBA administration, and mel efficiently reduced the prevalence of some aggressive tumors. Although mel promoted high EtOH consumption, it was effective in synchronizing the estrous cycle and reducing ovarian tumor mass by 20%. While rats in the C group displayed cysts containing serous fluid, C+EtOH rats showed solid tumor masses. After mel treatment, the ovaries of these rats presented as soft and mobile tissues. EtOH consumption increased the incidence of serous papillary carcinomas and sarcomas but not clear cell carcinomas. In contrast, mel reduced the incidence of sarcomas, endometrioid carcinomas and cystic teratomas. Combination of DMBA with EtOH intake potentiated the incidence of OC with malignant histologic subtypes. We concluded that mel reduces ovarian masses and the incidence of adenocarcinomas in ethanol-deprived rats.Luiz Gustavo A ChuffaBeatriz A Fioruci-FontanelliLeonardo O MendesWagner J FávaroPatricia Fernanda F PinheiroMarcelo MartinezFrancisco Eduardo MartinezPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e81676 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Luiz Gustavo A Chuffa Beatriz A Fioruci-Fontanelli Leonardo O Mendes Wagner J Fávaro Patricia Fernanda F Pinheiro Marcelo Martinez Francisco Eduardo Martinez Characterization of chemically induced ovarian carcinomas in an ethanol-preferring rat model: influence of long-term melatonin treatment.
description	Ovarian cancer is the fourth most common cause of cancer deaths among women, and chronic alcoholism may exert co-carcinogenic effects. Because melatonin (mel) has oncostatic properties, we aimed to investigate and characterize the chemical induction of ovarian tumors in a model of ethanol-preferring rats and to verify the influence of mel treatment on the overall features of these tumors. After rats were selected to receive ethanol (EtOH), they were surgically injected with 100 µg of 7,12-dimethyl-benz[a]anthracene (DMBA) plus sesame oil directly under the left ovarian bursa. At 260 days old, half of the animals received i.p. injections of 200 µg mel/100 g b.w. for 60 days. Four experimental groups were established: Group C, rats bearing ovarian carcinomas (OC); Group C+EtOH, rats voluntarily consuming 10% (v/v) EtOH and bearing OC; Group C+M, rats bearing OC and receiving mel; and Group C+EtOH+M, rats with OC consuming EtOH and receiving mel. Estrous cycle and nutritional parameters were evaluated, and anatomopathological analyses of the ovarian tumors were conducted. The incidence of ovarian tumors was higher in EtOH drinking animals 120 days post-DMBA administration, and mel efficiently reduced the prevalence of some aggressive tumors. Although mel promoted high EtOH consumption, it was effective in synchronizing the estrous cycle and reducing ovarian tumor mass by 20%. While rats in the C group displayed cysts containing serous fluid, C+EtOH rats showed solid tumor masses. After mel treatment, the ovaries of these rats presented as soft and mobile tissues. EtOH consumption increased the incidence of serous papillary carcinomas and sarcomas but not clear cell carcinomas. In contrast, mel reduced the incidence of sarcomas, endometrioid carcinomas and cystic teratomas. Combination of DMBA with EtOH intake potentiated the incidence of OC with malignant histologic subtypes. We concluded that mel reduces ovarian masses and the incidence of adenocarcinomas in ethanol-deprived rats.
format	article
author	Luiz Gustavo A Chuffa Beatriz A Fioruci-Fontanelli Leonardo O Mendes Wagner J Fávaro Patricia Fernanda F Pinheiro Marcelo Martinez Francisco Eduardo Martinez
author_facet	Luiz Gustavo A Chuffa Beatriz A Fioruci-Fontanelli Leonardo O Mendes Wagner J Fávaro Patricia Fernanda F Pinheiro Marcelo Martinez Francisco Eduardo Martinez
author_sort	Luiz Gustavo A Chuffa
title	Characterization of chemically induced ovarian carcinomas in an ethanol-preferring rat model: influence of long-term melatonin treatment.
title_short	Characterization of chemically induced ovarian carcinomas in an ethanol-preferring rat model: influence of long-term melatonin treatment.
title_full	Characterization of chemically induced ovarian carcinomas in an ethanol-preferring rat model: influence of long-term melatonin treatment.
title_fullStr	Characterization of chemically induced ovarian carcinomas in an ethanol-preferring rat model: influence of long-term melatonin treatment.
title_full_unstemmed	Characterization of chemically induced ovarian carcinomas in an ethanol-preferring rat model: influence of long-term melatonin treatment.
title_sort	characterization of chemically induced ovarian carcinomas in an ethanol-preferring rat model: influence of long-term melatonin treatment.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/27280d8f53a74b8eb1daf6b55bd82ae7
work_keys_str_mv	AT luizgustavoachuffa characterizationofchemicallyinducedovariancarcinomasinanethanolpreferringratmodelinfluenceoflongtermmelatonintreatment AT beatrizafiorucifontanelli characterizationofchemicallyinducedovariancarcinomasinanethanolpreferringratmodelinfluenceoflongtermmelatonintreatment AT leonardoomendes characterizationofchemicallyinducedovariancarcinomasinanethanolpreferringratmodelinfluenceoflongtermmelatonintreatment AT wagnerjfavaro characterizationofchemicallyinducedovariancarcinomasinanethanolpreferringratmodelinfluenceoflongtermmelatonintreatment AT patriciafernandafpinheiro characterizationofchemicallyinducedovariancarcinomasinanethanolpreferringratmodelinfluenceoflongtermmelatonintreatment AT marcelomartinez characterizationofchemicallyinducedovariancarcinomasinanethanolpreferringratmodelinfluenceoflongtermmelatonintreatment AT franciscoeduardomartinez characterizationofchemicallyinducedovariancarcinomasinanethanolpreferringratmodelinfluenceoflongtermmelatonintreatment
_version_	1718421433620627456

Characterization of chemically induced ovarian carcinomas in an ethanol-preferring rat model: influence of long-term melatonin treatment.

Ejemplares similares