Dynamics of Neutralizing Antibody Responses Following Natural SARS-CoV-2 Infection and Correlation with Commercial Serologic Tests. A Reappraisal and Indirect Comparison with Vaccinated Subjects

Neutralising antibodies (NAbs) represent the real source of protection against SARS-CoV-2 infections by preventing the virus from entering target cells. The gold standard in the detection of these antibodies is the plaque reduction neutralization test (PRNT). As these experiments must be done in a v...

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Autores principales:	Constant Gillot, Julien Favresse, Vincent Maloteau, Jean-Michel Dogné, Jonathan Douxfils
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	COVID-19 SARS-CoV-2 neutralizing antibody Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/27293ea28d8e46fc81c6f70d5abc2928
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spelling	oai:doaj.org-article:27293ea28d8e46fc81c6f70d5abc29282021-11-25T19:14:38ZDynamics of Neutralizing Antibody Responses Following Natural SARS-CoV-2 Infection and Correlation with Commercial Serologic Tests. A Reappraisal and Indirect Comparison with Vaccinated Subjects10.3390/v131123291999-4915https://doaj.org/article/27293ea28d8e46fc81c6f70d5abc29282021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2329https://doaj.org/toc/1999-4915Neutralising antibodies (NAbs) represent the real source of protection against SARS-CoV-2 infections by preventing the virus from entering target cells. The gold standard in the detection of these antibodies is the plaque reduction neutralization test (PRNT). As these experiments must be done in a very secure environment, other techniques based on pseudoviruses: pseudovirus neutralization test (pVNT) or surrogate virus neutralization test (sVNT) have been developed. Binding assays, on the other hand, measure total antibodies or IgG, IgM, and IgA directed against one epitope of the SARS-CoV-2, independently of their neutralizing capacity. The aim of this study is to compare the performance of six commercial binding assays to the pVNT and sVNT. In this study, we used blood samples from a cohort of 62 RT-PCR confirmed COVID-19 patients. Based on the results of the neutralizing assays, adapted cut-offs for the binding assays were calculated. The use of these adapted cut-offs does not permit to improve the accuracy of the serological assays and we did not find an adapted cut-off able to improve the capacity of these tests to detect NAbs. For a part of the population, a longitudinal follow-up with at least two samples for the same patient was performed. From day 14 to day 291, more than 75% of the samples were positive for NAbs (<i>n</i> = 87/110, 79.1%). Interestingly, 6 months post symptoms onset, the majority of the samples (<i>N</i> = 44/52, 84.6%) were still positive for NAbs. This is in sharp contrast with the results we obtained 6 months post-vaccination in our cohort of healthcare workers who have received the two-dose regimens of BNT162b2. In this cohort of vaccinated subjects, 43% (<i>n</i> = 25/58) of the participants no longer exhibit NAbs activity 180 days after the administration of the first dose of BNT162b2.Constant GillotJulien FavresseVincent MaloteauJean-Michel DognéJonathan DouxfilsMDPI AGarticleCOVID-19SARS-CoV-2neutralizing antibodyMicrobiologyQR1-502ENViruses, Vol 13, Iss 2329, p 2329 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	COVID-19 SARS-CoV-2 neutralizing antibody Microbiology QR1-502
spellingShingle	COVID-19 SARS-CoV-2 neutralizing antibody Microbiology QR1-502 Constant Gillot Julien Favresse Vincent Maloteau Jean-Michel Dogné Jonathan Douxfils Dynamics of Neutralizing Antibody Responses Following Natural SARS-CoV-2 Infection and Correlation with Commercial Serologic Tests. A Reappraisal and Indirect Comparison with Vaccinated Subjects
description	Neutralising antibodies (NAbs) represent the real source of protection against SARS-CoV-2 infections by preventing the virus from entering target cells. The gold standard in the detection of these antibodies is the plaque reduction neutralization test (PRNT). As these experiments must be done in a very secure environment, other techniques based on pseudoviruses: pseudovirus neutralization test (pVNT) or surrogate virus neutralization test (sVNT) have been developed. Binding assays, on the other hand, measure total antibodies or IgG, IgM, and IgA directed against one epitope of the SARS-CoV-2, independently of their neutralizing capacity. The aim of this study is to compare the performance of six commercial binding assays to the pVNT and sVNT. In this study, we used blood samples from a cohort of 62 RT-PCR confirmed COVID-19 patients. Based on the results of the neutralizing assays, adapted cut-offs for the binding assays were calculated. The use of these adapted cut-offs does not permit to improve the accuracy of the serological assays and we did not find an adapted cut-off able to improve the capacity of these tests to detect NAbs. For a part of the population, a longitudinal follow-up with at least two samples for the same patient was performed. From day 14 to day 291, more than 75% of the samples were positive for NAbs (<i>n</i> = 87/110, 79.1%). Interestingly, 6 months post symptoms onset, the majority of the samples (<i>N</i> = 44/52, 84.6%) were still positive for NAbs. This is in sharp contrast with the results we obtained 6 months post-vaccination in our cohort of healthcare workers who have received the two-dose regimens of BNT162b2. In this cohort of vaccinated subjects, 43% (<i>n</i> = 25/58) of the participants no longer exhibit NAbs activity 180 days after the administration of the first dose of BNT162b2.
format	article
author	Constant Gillot Julien Favresse Vincent Maloteau Jean-Michel Dogné Jonathan Douxfils
author_facet	Constant Gillot Julien Favresse Vincent Maloteau Jean-Michel Dogné Jonathan Douxfils
author_sort	Constant Gillot
title	Dynamics of Neutralizing Antibody Responses Following Natural SARS-CoV-2 Infection and Correlation with Commercial Serologic Tests. A Reappraisal and Indirect Comparison with Vaccinated Subjects
title_short	Dynamics of Neutralizing Antibody Responses Following Natural SARS-CoV-2 Infection and Correlation with Commercial Serologic Tests. A Reappraisal and Indirect Comparison with Vaccinated Subjects
title_full	Dynamics of Neutralizing Antibody Responses Following Natural SARS-CoV-2 Infection and Correlation with Commercial Serologic Tests. A Reappraisal and Indirect Comparison with Vaccinated Subjects
title_fullStr	Dynamics of Neutralizing Antibody Responses Following Natural SARS-CoV-2 Infection and Correlation with Commercial Serologic Tests. A Reappraisal and Indirect Comparison with Vaccinated Subjects
title_full_unstemmed	Dynamics of Neutralizing Antibody Responses Following Natural SARS-CoV-2 Infection and Correlation with Commercial Serologic Tests. A Reappraisal and Indirect Comparison with Vaccinated Subjects
title_sort	dynamics of neutralizing antibody responses following natural sars-cov-2 infection and correlation with commercial serologic tests. a reappraisal and indirect comparison with vaccinated subjects
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/27293ea28d8e46fc81c6f70d5abc2928
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Dynamics of Neutralizing Antibody Responses Following Natural SARS-CoV-2 Infection and Correlation with Commercial Serologic Tests. A Reappraisal and Indirect Comparison with Vaccinated Subjects

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