Selective algicidal action of peptides against harmful algal bloom species.

Recently, harmful algal bloom (HAB), also termed "red tide", has been recognized as a serious problem in marine environments according to climate changes worldwide. Many novel materials or methods to prevent HAB have not yet been employed except for clay dispersion, in which can the result...

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Autores principales:	Seong-Cheol Park, Jong-Kook Lee, Si Wouk Kim, Yoonkyung Park
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2729d2296cc14efeb9ee3b96e2e3485a
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spelling	oai:doaj.org-article:2729d2296cc14efeb9ee3b96e2e3485a2021-11-18T07:35:42ZSelective algicidal action of peptides against harmful algal bloom species.1932-620310.1371/journal.pone.0026733https://doaj.org/article/2729d2296cc14efeb9ee3b96e2e3485a2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22046341/?tool=EBIhttps://doaj.org/toc/1932-6203Recently, harmful algal bloom (HAB), also termed "red tide", has been recognized as a serious problem in marine environments according to climate changes worldwide. Many novel materials or methods to prevent HAB have not yet been employed except for clay dispersion, in which can the resulting sedimentation on the seafloor can also cause alteration in marine ecology or secondary environmental pollution. In the current study, we investigated that antimicrobial peptide have a potential in controlling HAB without cytotoxicity to harmless marine organisms. Here, antimicrobial peptides are proposed as new algicidal compounds in combating HAB cells. HPA3 and HPA3NT3 peptides which exert potent antimicrobial activity via pore forming action in plasma membrane showed that HPA3NT3 reduced the motility of algal cells, disrupted their plasma membrane, and induced the efflux of intracellular components. Against raphidoflagellate such as Heterosigma akashiwo, Chattonella sp., and C. marina, it displayed a rapid lysing action in cell membranes at 1~4 µM within 2 min. Comparatively, its lysing effects occurred at 8 µM within 1 h in dinoflagellate such as Cochlodium polykrikoides, Prorocentrum micans, and P. minimum. Moreover, its lysing action induced the lysis of chloroplasts and loss of chlorophyll a. In the contrary, this peptide was not effective against Skeletonema costatum, harmless algal cell, even at 256 µM, moreover, it killed only H. akashiwo or C. marina in co-cultivation with S. costatum, indicating to its selective algicidal activity between harmful and harmless algal cells. The peptide was non-hemolytic against red blood cells of Sebastes schlegeli, the black rockfish, at 120 µM. HAB cells were quickly and selectively lysed following treatment of antimicrobial peptides without cytotoxicity to harmless marine organisms. Thus, the antibiotic peptides examined in our study appear to have much potential in effectively controlling HAB with minimal impact on marine ecology.Seong-Cheol ParkJong-Kook LeeSi Wouk KimYoonkyung ParkPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 10, p e26733 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Seong-Cheol Park Jong-Kook Lee Si Wouk Kim Yoonkyung Park Selective algicidal action of peptides against harmful algal bloom species.
description	Recently, harmful algal bloom (HAB), also termed "red tide", has been recognized as a serious problem in marine environments according to climate changes worldwide. Many novel materials or methods to prevent HAB have not yet been employed except for clay dispersion, in which can the resulting sedimentation on the seafloor can also cause alteration in marine ecology or secondary environmental pollution. In the current study, we investigated that antimicrobial peptide have a potential in controlling HAB without cytotoxicity to harmless marine organisms. Here, antimicrobial peptides are proposed as new algicidal compounds in combating HAB cells. HPA3 and HPA3NT3 peptides which exert potent antimicrobial activity via pore forming action in plasma membrane showed that HPA3NT3 reduced the motility of algal cells, disrupted their plasma membrane, and induced the efflux of intracellular components. Against raphidoflagellate such as Heterosigma akashiwo, Chattonella sp., and C. marina, it displayed a rapid lysing action in cell membranes at 1~4 µM within 2 min. Comparatively, its lysing effects occurred at 8 µM within 1 h in dinoflagellate such as Cochlodium polykrikoides, Prorocentrum micans, and P. minimum. Moreover, its lysing action induced the lysis of chloroplasts and loss of chlorophyll a. In the contrary, this peptide was not effective against Skeletonema costatum, harmless algal cell, even at 256 µM, moreover, it killed only H. akashiwo or C. marina in co-cultivation with S. costatum, indicating to its selective algicidal activity between harmful and harmless algal cells. The peptide was non-hemolytic against red blood cells of Sebastes schlegeli, the black rockfish, at 120 µM. HAB cells were quickly and selectively lysed following treatment of antimicrobial peptides without cytotoxicity to harmless marine organisms. Thus, the antibiotic peptides examined in our study appear to have much potential in effectively controlling HAB with minimal impact on marine ecology.
format	article
author	Seong-Cheol Park Jong-Kook Lee Si Wouk Kim Yoonkyung Park
author_facet	Seong-Cheol Park Jong-Kook Lee Si Wouk Kim Yoonkyung Park
author_sort	Seong-Cheol Park
title	Selective algicidal action of peptides against harmful algal bloom species.
title_short	Selective algicidal action of peptides against harmful algal bloom species.
title_full	Selective algicidal action of peptides against harmful algal bloom species.
title_fullStr	Selective algicidal action of peptides against harmful algal bloom species.
title_full_unstemmed	Selective algicidal action of peptides against harmful algal bloom species.
title_sort	selective algicidal action of peptides against harmful algal bloom species.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/2729d2296cc14efeb9ee3b96e2e3485a
work_keys_str_mv	AT seongcheolpark selectivealgicidalactionofpeptidesagainstharmfulalgalbloomspecies AT jongkooklee selectivealgicidalactionofpeptidesagainstharmfulalgalbloomspecies AT siwoukkim selectivealgicidalactionofpeptidesagainstharmfulalgalbloomspecies AT yoonkyungpark selectivealgicidalactionofpeptidesagainstharmfulalgalbloomspecies
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Selective algicidal action of peptides against harmful algal bloom species.

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