Molecular Characterization of Viral Responsive Protein 15 and Its Possible Role in Nuclear Export of Virus in Black Tiger Shrimp Penaeus monodon

Abstract A viral responsive protein 15 from Penaeus monodon (PmVRP15) has been reported to be important for white spot syndrome virus (WSSV) infection in vivo. This work aims to characterize PmVRP15 and investigate its possible role in nuclear import/export of the virus. Circular dichroism spectra s...

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Autores principales:	Krisadaporn Jaturontakul, Thapanan Jatuyosporn, Pasunee Laohawutthichai, Sun-Yong Kim, Tomoyuki Mori, Premruethai Supungul, Toshio Hakoshima, Anchalee Tassanakajon, Kuakarun Krusong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/273251378f494a37aa3d54cc76b1fa9e
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spelling	oai:doaj.org-article:273251378f494a37aa3d54cc76b1fa9e2021-12-02T11:52:40ZMolecular Characterization of Viral Responsive Protein 15 and Its Possible Role in Nuclear Export of Virus in Black Tiger Shrimp Penaeus monodon10.1038/s41598-017-06653-72045-2322https://doaj.org/article/273251378f494a37aa3d54cc76b1fa9e2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06653-7https://doaj.org/toc/2045-2322Abstract A viral responsive protein 15 from Penaeus monodon (PmVRP15) has been reported to be important for white spot syndrome virus (WSSV) infection in vivo. This work aims to characterize PmVRP15 and investigate its possible role in nuclear import/export of the virus. Circular dichroism spectra showed that PmVRP15 contains high helical contents (82%). Analytical ultracentrifugation suggested that PmVRP15 could possibly form oligomers in solution. A subcellular fractionation study showed that PmVRP15 was found in heavy and light membrane fractions, indicating that PmVRP15 may be associated with endoplasmic reticulum. Double-stranded RNAi-mediated knockdown of PmVRP15 gene expression in vitro showed no effect on WSSV copy number in whole hemocyte cells. However, PmVRP15 silencing resulted in an accumulation of WSSV DNA in the nucleus of PmVRP15-silenced hemocytes. Immunofluorescence confocal microscopy showed that PmVRP15 knockdown hemocytes had a much lower level of VP28 (WSSV envelope protein), in comparison to that in the control. It is likely that PmVRP15 may play a role in viral nuclear egress.Krisadaporn JaturontakulThapanan JatuyospornPasunee LaohawutthichaiSun-Yong KimTomoyuki MoriPremruethai SupungulToshio HakoshimaAnchalee TassanakajonKuakarun KrusongNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Krisadaporn Jaturontakul Thapanan Jatuyosporn Pasunee Laohawutthichai Sun-Yong Kim Tomoyuki Mori Premruethai Supungul Toshio Hakoshima Anchalee Tassanakajon Kuakarun Krusong Molecular Characterization of Viral Responsive Protein 15 and Its Possible Role in Nuclear Export of Virus in Black Tiger Shrimp Penaeus monodon
description	Abstract A viral responsive protein 15 from Penaeus monodon (PmVRP15) has been reported to be important for white spot syndrome virus (WSSV) infection in vivo. This work aims to characterize PmVRP15 and investigate its possible role in nuclear import/export of the virus. Circular dichroism spectra showed that PmVRP15 contains high helical contents (82%). Analytical ultracentrifugation suggested that PmVRP15 could possibly form oligomers in solution. A subcellular fractionation study showed that PmVRP15 was found in heavy and light membrane fractions, indicating that PmVRP15 may be associated with endoplasmic reticulum. Double-stranded RNAi-mediated knockdown of PmVRP15 gene expression in vitro showed no effect on WSSV copy number in whole hemocyte cells. However, PmVRP15 silencing resulted in an accumulation of WSSV DNA in the nucleus of PmVRP15-silenced hemocytes. Immunofluorescence confocal microscopy showed that PmVRP15 knockdown hemocytes had a much lower level of VP28 (WSSV envelope protein), in comparison to that in the control. It is likely that PmVRP15 may play a role in viral nuclear egress.
format	article
author	Krisadaporn Jaturontakul Thapanan Jatuyosporn Pasunee Laohawutthichai Sun-Yong Kim Tomoyuki Mori Premruethai Supungul Toshio Hakoshima Anchalee Tassanakajon Kuakarun Krusong
author_facet	Krisadaporn Jaturontakul Thapanan Jatuyosporn Pasunee Laohawutthichai Sun-Yong Kim Tomoyuki Mori Premruethai Supungul Toshio Hakoshima Anchalee Tassanakajon Kuakarun Krusong
author_sort	Krisadaporn Jaturontakul
title	Molecular Characterization of Viral Responsive Protein 15 and Its Possible Role in Nuclear Export of Virus in Black Tiger Shrimp Penaeus monodon
title_short	Molecular Characterization of Viral Responsive Protein 15 and Its Possible Role in Nuclear Export of Virus in Black Tiger Shrimp Penaeus monodon
title_full	Molecular Characterization of Viral Responsive Protein 15 and Its Possible Role in Nuclear Export of Virus in Black Tiger Shrimp Penaeus monodon
title_fullStr	Molecular Characterization of Viral Responsive Protein 15 and Its Possible Role in Nuclear Export of Virus in Black Tiger Shrimp Penaeus monodon
title_full_unstemmed	Molecular Characterization of Viral Responsive Protein 15 and Its Possible Role in Nuclear Export of Virus in Black Tiger Shrimp Penaeus monodon
title_sort	molecular characterization of viral responsive protein 15 and its possible role in nuclear export of virus in black tiger shrimp penaeus monodon
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/273251378f494a37aa3d54cc76b1fa9e
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Molecular Characterization of Viral Responsive Protein 15 and Its Possible Role in Nuclear Export of Virus in Black Tiger Shrimp Penaeus monodon

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