Doxycycline in Extremely Low Dose Improves Glycemic Control and Islet Morphology in Mice Fed a High-Fat Diet

Yixin Chen,1 Yu Chen,2 Na Wang,2 Shanhong Gu,1 Meilin Wang,1 Yucai Fu,3 Chiju Wei,2 Wencan Xu1 1Department of Endocrinology, The First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, 515041, People’s Republic of China; 2Laboratory of Molecular Biology, Multid...

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Autores principales:	Chen Y, Wang N, Gu S, Wang M, Fu Y, Wei C, Xu W
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	doxycycline inflammation t2dm diabetes glucose metabolism Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/27385cf3cbba43a6b57878c37e2bcab1
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spelling	oai:doaj.org-article:27385cf3cbba43a6b57878c37e2bcab12021-12-02T13:30:40ZDoxycycline in Extremely Low Dose Improves Glycemic Control and Islet Morphology in Mice Fed a High-Fat Diet1178-7007https://doaj.org/article/27385cf3cbba43a6b57878c37e2bcab12021-02-01T00:00:00Zhttps://www.dovepress.com/doxycycline-in-extremely-low-dose-improves-glycemic-control-and-islet--peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Yixin Chen,1 Yu Chen,2 Na Wang,2 Shanhong Gu,1 Meilin Wang,1 Yucai Fu,3 Chiju Wei,2 Wencan Xu1 1Department of Endocrinology, The First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, 515041, People’s Republic of China; 2Laboratory of Molecular Biology, Multidisciplinary Research Center, Shantou University, Shantou, Guangdong, 515063, People’s Republic of China; 3Laboratory of Cell Senescence, Shantou University Medical College, Shantou, Guangdong, 515041, People’s Republic of ChinaCorrespondence: Chiju WeiShantou University, 243 Daxue Road, Shantou, Guangdong, 515063, People’s Republic of ChinaEmail chijuwei@stu.edu.cnWencan XuDepartment of Endocrinology, The First Affiliated Hospital of Shantou University Medical College, 57 Changping Road, Shantou, Guangdong, 515041, People’s Republic of ChinaEmail xuwcan@163.comPurpose: Chronic low-grade inflammation is detected in obese and diabetic individuals. Tetracyclines, used as antibiotics for years, have been demonstrated to have diverse non-bactericidal effects, including anti-tumor and anti-inflammatory activities. This study aimed to investigate whether doxycycline at sub-antimicrobial concentrations could improve glycemic control in mice fed a high-fat diet, through its anti-inflammatory activities.Methods: C57BL/6J mice were fed with a high-fat diet to induce diabetic and obese conditions. Three sub-antimicrobial dosages of doxycycline (200, 20, and 2 μg/mL) were added to drinking water for 23 weeks during the housing phase.Results: Doxycycline at 200 μg/mL tended to increase body weight, islet mass, and the percentage of large islets (diameter > 350 μm). At 20 μg/mL, doxycycline significantly improved glucose tolerance and decreased fasting blood glucose. At 2 μg/mL, doxycycline increased the percentage of small islets (diameter < 80 μm). Serum C-reactive protein and lipopolysaccharide levels significantly decreased while the beta-cell ratio increased in all doxycycline-administered mice.Conclusion: Our results suggest that doxycycline, even at an extremely low dose, could improve glycemic control and islet morphology via its anti-inflammatory activities.Keywords: doxycycline, inflammation, T2DM, diabetes, glucose metabolismChen YChen YWang NGu SWang MFu YWei CXu WDove Medical Pressarticledoxycyclineinflammationt2dmdiabetesglucose metabolismSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 14, Pp 637-646 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	doxycycline inflammation t2dm diabetes glucose metabolism Specialties of internal medicine RC581-951
spellingShingle	doxycycline inflammation t2dm diabetes glucose metabolism Specialties of internal medicine RC581-951 Chen Y Chen Y Wang N Gu S Wang M Fu Y Wei C Xu W Doxycycline in Extremely Low Dose Improves Glycemic Control and Islet Morphology in Mice Fed a High-Fat Diet
description	Yixin Chen,1 Yu Chen,2 Na Wang,2 Shanhong Gu,1 Meilin Wang,1 Yucai Fu,3 Chiju Wei,2 Wencan Xu1 1Department of Endocrinology, The First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong, 515041, People’s Republic of China; 2Laboratory of Molecular Biology, Multidisciplinary Research Center, Shantou University, Shantou, Guangdong, 515063, People’s Republic of China; 3Laboratory of Cell Senescence, Shantou University Medical College, Shantou, Guangdong, 515041, People’s Republic of ChinaCorrespondence: Chiju WeiShantou University, 243 Daxue Road, Shantou, Guangdong, 515063, People’s Republic of ChinaEmail chijuwei@stu.edu.cnWencan XuDepartment of Endocrinology, The First Affiliated Hospital of Shantou University Medical College, 57 Changping Road, Shantou, Guangdong, 515041, People’s Republic of ChinaEmail xuwcan@163.comPurpose: Chronic low-grade inflammation is detected in obese and diabetic individuals. Tetracyclines, used as antibiotics for years, have been demonstrated to have diverse non-bactericidal effects, including anti-tumor and anti-inflammatory activities. This study aimed to investigate whether doxycycline at sub-antimicrobial concentrations could improve glycemic control in mice fed a high-fat diet, through its anti-inflammatory activities.Methods: C57BL/6J mice were fed with a high-fat diet to induce diabetic and obese conditions. Three sub-antimicrobial dosages of doxycycline (200, 20, and 2 μg/mL) were added to drinking water for 23 weeks during the housing phase.Results: Doxycycline at 200 μg/mL tended to increase body weight, islet mass, and the percentage of large islets (diameter > 350 μm). At 20 μg/mL, doxycycline significantly improved glucose tolerance and decreased fasting blood glucose. At 2 μg/mL, doxycycline increased the percentage of small islets (diameter < 80 μm). Serum C-reactive protein and lipopolysaccharide levels significantly decreased while the beta-cell ratio increased in all doxycycline-administered mice.Conclusion: Our results suggest that doxycycline, even at an extremely low dose, could improve glycemic control and islet morphology via its anti-inflammatory activities.Keywords: doxycycline, inflammation, T2DM, diabetes, glucose metabolism
format	article
author	Chen Y Chen Y Wang N Gu S Wang M Fu Y Wei C Xu W
author_facet	Chen Y Chen Y Wang N Gu S Wang M Fu Y Wei C Xu W
author_sort	Chen Y
title	Doxycycline in Extremely Low Dose Improves Glycemic Control and Islet Morphology in Mice Fed a High-Fat Diet
title_short	Doxycycline in Extremely Low Dose Improves Glycemic Control and Islet Morphology in Mice Fed a High-Fat Diet
title_full	Doxycycline in Extremely Low Dose Improves Glycemic Control and Islet Morphology in Mice Fed a High-Fat Diet
title_fullStr	Doxycycline in Extremely Low Dose Improves Glycemic Control and Islet Morphology in Mice Fed a High-Fat Diet
title_full_unstemmed	Doxycycline in Extremely Low Dose Improves Glycemic Control and Islet Morphology in Mice Fed a High-Fat Diet
title_sort	doxycycline in extremely low dose improves glycemic control and islet morphology in mice fed a high-fat diet
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/27385cf3cbba43a6b57878c37e2bcab1
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Doxycycline in Extremely Low Dose Improves Glycemic Control and Islet Morphology in Mice Fed a High-Fat Diet

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