Diagnosing bluetongue virus in domestic ruminants: current perspectives

José M Rojas, Daniel Rodríguez-Martín, Verónica Martín, Noemí Sevilla Centro de Investigación en Sanidad Animal (CISA-INIA), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Vald...

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Autores principales:	Rojas JM, Rodríguez-Martín D, Martín V, Sevilla N
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2019
Materias:	Bluetongue virus Diagnostic tests Virus isolation serological tools Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/273c88aadb8949969e62ee5b776c5fc2
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spelling	oai:doaj.org-article:273c88aadb8949969e62ee5b776c5fc22021-12-02T04:13:03ZDiagnosing bluetongue virus in domestic ruminants: current perspectives2230-2034https://doaj.org/article/273c88aadb8949969e62ee5b776c5fc22019-02-01T00:00:00Zhttps://www.dovepress.com/diagnosing-bluetongue-virus-in-domestic-ruminants-current-perspectives-peer-reviewed-article-VMRRhttps://doaj.org/toc/2230-2034José M Rojas, Daniel Rodríguez-Martín, Verónica Martín, Noemí Sevilla Centro de Investigación en Sanidad Animal (CISA-INIA), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Valdeolmos, Madrid, Spain Abstract:This review provides an overview of current and potential new diagnostic techniques against bluetongue virus (BTV), an Orbivirus transmitted by arthropods that affects ruminants. Bluetongue is a disease currently notifiable to the World Organization for Animal Health (OIE), causing great economic losses due to decreased trade associated with bluetongue outbreaks and high mortality and morbidity. BTV cross-reacts with many antigenically related viruses including viruses that causes African Horse sickness and epizootic haemorrhagic disease of deer. Therefore, reliable diagnostic approaches to detect BTV among these other antigenically related viruses are used or being developed. The antigenic determinant for differentiation of virus species/serogroups among orbiviruses is the VP7 protein, meanwhile VP2 is serotype specific. Serologically, assays are established in many laboratories, based mainly on competitive ELISA or serum neutralization assay (virus neutralization assay [VNT]) although new techniques are being developed. Virus isolation from blood or semen is, additionally, another means of BTV diagnosis. Nevertheless, most of these techniques for viral isolation are time-consuming and expensive. Currently, reverse-transcription polymerase chain reaction (RT-PCR) panels or real-time RT-PCR are widely used methods although next-generation sequencing remains of interest for future virus diagnosis. Keywords: bluetongue virus, diagnostic tests, virus isolation, serological tools  Rojas JMRodríguez-Martín DMartín VSevilla NDove Medical PressarticleBluetongue virusDiagnostic testsVirus isolationserological toolsVeterinary medicineSF600-1100ENVeterinary Medicine: Research and Reports, Vol Volume 10, Pp 17-27 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Bluetongue virus Diagnostic tests Virus isolation serological tools Veterinary medicine SF600-1100
spellingShingle	Bluetongue virus Diagnostic tests Virus isolation serological tools Veterinary medicine SF600-1100 Rojas JM Rodríguez-Martín D Martín V Sevilla N Diagnosing bluetongue virus in domestic ruminants: current perspectives
description	José M Rojas, Daniel Rodríguez-Martín, Verónica Martín, Noemí Sevilla Centro de Investigación en Sanidad Animal (CISA-INIA), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria, Valdeolmos, Madrid, Spain Abstract:This review provides an overview of current and potential new diagnostic techniques against bluetongue virus (BTV), an Orbivirus transmitted by arthropods that affects ruminants. Bluetongue is a disease currently notifiable to the World Organization for Animal Health (OIE), causing great economic losses due to decreased trade associated with bluetongue outbreaks and high mortality and morbidity. BTV cross-reacts with many antigenically related viruses including viruses that causes African Horse sickness and epizootic haemorrhagic disease of deer. Therefore, reliable diagnostic approaches to detect BTV among these other antigenically related viruses are used or being developed. The antigenic determinant for differentiation of virus species/serogroups among orbiviruses is the VP7 protein, meanwhile VP2 is serotype specific. Serologically, assays are established in many laboratories, based mainly on competitive ELISA or serum neutralization assay (virus neutralization assay [VNT]) although new techniques are being developed. Virus isolation from blood or semen is, additionally, another means of BTV diagnosis. Nevertheless, most of these techniques for viral isolation are time-consuming and expensive. Currently, reverse-transcription polymerase chain reaction (RT-PCR) panels or real-time RT-PCR are widely used methods although next-generation sequencing remains of interest for future virus diagnosis. Keywords: bluetongue virus, diagnostic tests, virus isolation, serological tools
format	article
author	Rojas JM Rodríguez-Martín D Martín V Sevilla N
author_facet	Rojas JM Rodríguez-Martín D Martín V Sevilla N
author_sort	Rojas JM
title	Diagnosing bluetongue virus in domestic ruminants: current perspectives
title_short	Diagnosing bluetongue virus in domestic ruminants: current perspectives
title_full	Diagnosing bluetongue virus in domestic ruminants: current perspectives
title_fullStr	Diagnosing bluetongue virus in domestic ruminants: current perspectives
title_full_unstemmed	Diagnosing bluetongue virus in domestic ruminants: current perspectives
title_sort	diagnosing bluetongue virus in domestic ruminants: current perspectives
publisher	Dove Medical Press
publishDate	2019
url	https://doaj.org/article/273c88aadb8949969e62ee5b776c5fc2
work_keys_str_mv	AT rojasjm diagnosingbluetonguevirusindomesticruminantscurrentperspectives AT rodriguezmartind diagnosingbluetonguevirusindomesticruminantscurrentperspectives AT martinv diagnosingbluetonguevirusindomesticruminantscurrentperspectives AT sevillan diagnosingbluetonguevirusindomesticruminantscurrentperspectives
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Diagnosing bluetongue virus in domestic ruminants: current perspectives

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