Comparison of N-acetyl-L-cysteine-sodium hydroxide and Modified Petroff’s Decontamination Methods for Mycobacterium tuberculosis Culture

Indonesia is one of 22 countries with a high incidence of TB in the world, particularly related to TB-HIV and MDR-TB cases. Contamination of normal flora from nasopharyngeal tract is the main problem to isolate Mycobacterium tuberculosis (MTB) from sputum. It is needed a safe solution to decontam...

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Autores principales:	ARIYANI KIRANASARI, MUHAMMAD RAYHAN
Formato:	article
Lenguaje:	EN
Publicado:	Indonesian Society for Microbiology 2021
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/274620dfe1194f06ab648b272eb28fe3
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spelling	oai:doaj.org-article:274620dfe1194f06ab648b272eb28fe32021-11-09T06:15:13ZComparison of N-acetyl-L-cysteine-sodium hydroxide and Modified Petroff’s Decontamination Methods for Mycobacterium tuberculosis Culture10.5454/mi.15.3.51978-34772087-8575https://doaj.org/article/274620dfe1194f06ab648b272eb28fe32021-10-01T00:00:00Zhttps://jurnal.permi.or.id/index.php/mionline/article/view/851https://doaj.org/toc/1978-3477https://doaj.org/toc/2087-8575 Indonesia is one of 22 countries with a high incidence of TB in the world, particularly related to TB-HIV and MDR-TB cases. Contamination of normal flora from nasopharyngeal tract is the main problem to isolate Mycobacterium tuberculosis (MTB) from sputum. It is needed a safe solution to decontaminate without killing MTB bacilli. N-acetyl-L-cysteine-sodium hydroxide (NALC-NaOH) is compared with modified Petroff’s which is widely used in laboratories, to get better outcome. Of the 110 sputum samples were collected from suspected cases of Pulmonary TB. Decontamination using 2% NALC-NaOH and Modifed Petroff (4% NaOH) was performed before AFB smear and culture on LJ medium. Each group was assessed for contamination and culture positive rate. The positive culture was validated using chromatography test for detected antigen MPT-64 and PNB. Result of this study showed that NALC-NaOH and modified Petroff’s methods did not significantly affect positivity rate of Acid-Fast Bacillus (AFB) smear, 71% and 66% respectively. Contamination on culture was significantly (p=0,034) higher in samples treated with NALC-NaOH (21%) compared to Modified Petroff methods(13%). Proportion of positive culture on samples treated with NALC-NaOH was lower than Modified Petroff, 65% and 70% respectively with p value=1. Conclusion of this study showed that sputum decontamination using Modified Petroff methods is still more effective than NALC-NaOH to increase positivity rate of MTB culture. However, the two methods were not significantly different to get positivity result on the microscopic examination of AFB. ARIYANI KIRANASARIMUHAMMAD RAYHANIndonesian Society for MicrobiologyarticleMicrobiologyQR1-502ENMicrobiology Indonesia, Vol 15, Iss 3 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 ARIYANI KIRANASARI MUHAMMAD RAYHAN Comparison of N-acetyl-L-cysteine-sodium hydroxide and Modified Petroff’s Decontamination Methods for Mycobacterium tuberculosis Culture
description	Indonesia is one of 22 countries with a high incidence of TB in the world, particularly related to TB-HIV and MDR-TB cases. Contamination of normal flora from nasopharyngeal tract is the main problem to isolate Mycobacterium tuberculosis (MTB) from sputum. It is needed a safe solution to decontaminate without killing MTB bacilli. N-acetyl-L-cysteine-sodium hydroxide (NALC-NaOH) is compared with modified Petroff’s which is widely used in laboratories, to get better outcome. Of the 110 sputum samples were collected from suspected cases of Pulmonary TB. Decontamination using 2% NALC-NaOH and Modifed Petroff (4% NaOH) was performed before AFB smear and culture on LJ medium. Each group was assessed for contamination and culture positive rate. The positive culture was validated using chromatography test for detected antigen MPT-64 and PNB. Result of this study showed that NALC-NaOH and modified Petroff’s methods did not significantly affect positivity rate of Acid-Fast Bacillus (AFB) smear, 71% and 66% respectively. Contamination on culture was significantly (p=0,034) higher in samples treated with NALC-NaOH (21%) compared to Modified Petroff methods(13%). Proportion of positive culture on samples treated with NALC-NaOH was lower than Modified Petroff, 65% and 70% respectively with p value=1. Conclusion of this study showed that sputum decontamination using Modified Petroff methods is still more effective than NALC-NaOH to increase positivity rate of MTB culture. However, the two methods were not significantly different to get positivity result on the microscopic examination of AFB.
format	article
author	ARIYANI KIRANASARI MUHAMMAD RAYHAN
author_facet	ARIYANI KIRANASARI MUHAMMAD RAYHAN
author_sort	ARIYANI KIRANASARI
title	Comparison of N-acetyl-L-cysteine-sodium hydroxide and Modified Petroff’s Decontamination Methods for Mycobacterium tuberculosis Culture
title_short	Comparison of N-acetyl-L-cysteine-sodium hydroxide and Modified Petroff’s Decontamination Methods for Mycobacterium tuberculosis Culture
title_full	Comparison of N-acetyl-L-cysteine-sodium hydroxide and Modified Petroff’s Decontamination Methods for Mycobacterium tuberculosis Culture
title_fullStr	Comparison of N-acetyl-L-cysteine-sodium hydroxide and Modified Petroff’s Decontamination Methods for Mycobacterium tuberculosis Culture
title_full_unstemmed	Comparison of N-acetyl-L-cysteine-sodium hydroxide and Modified Petroff’s Decontamination Methods for Mycobacterium tuberculosis Culture
title_sort	comparison of n-acetyl-l-cysteine-sodium hydroxide and modified petroff’s decontamination methods for mycobacterium tuberculosis culture
publisher	Indonesian Society for Microbiology
publishDate	2021
url	https://doaj.org/article/274620dfe1194f06ab648b272eb28fe3
work_keys_str_mv	AT ariyanikiranasari comparisonofnacetyllcysteinesodiumhydroxideandmodifiedpetroffsdecontaminationmethodsformycobacteriumtuberculosisculture AT muhammadrayhan comparisonofnacetyllcysteinesodiumhydroxideandmodifiedpetroffsdecontaminationmethodsformycobacteriumtuberculosisculture
_version_	1718441279021383680

Comparison of N-acetyl-L-cysteine-sodium hydroxide and Modified Petroff’s Decontamination Methods for Mycobacterium tuberculosis Culture

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