Mechanism of Photodamage of the Oxygen Evolving Mn Cluster of Photosystem II by Excessive Light Energy

Abstract Photodamage to Photosystem II (PSII) has been attributed either to excessive excitation of photosynthetic pigments or by direct of light absorption by Mn4CaO5 cluster. Here we investigated the time course of PSII photodamage and release of Mn in PSII-enriched membranes under high light illu...

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Autores principales:	Alonso Zavafer, Wataru Koinuma, Wah Soon Chow, Mun Hon Cheah, Hiroyuki Mino
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2748f5a9514b4770af0d6004dd637a05
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spelling	oai:doaj.org-article:2748f5a9514b4770af0d6004dd637a052021-12-02T15:05:18ZMechanism of Photodamage of the Oxygen Evolving Mn Cluster of Photosystem II by Excessive Light Energy10.1038/s41598-017-07671-12045-2322https://doaj.org/article/2748f5a9514b4770af0d6004dd637a052017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07671-1https://doaj.org/toc/2045-2322Abstract Photodamage to Photosystem II (PSII) has been attributed either to excessive excitation of photosynthetic pigments or by direct of light absorption by Mn4CaO5 cluster. Here we investigated the time course of PSII photodamage and release of Mn in PSII-enriched membranes under high light illumination at 460 nm and 660 nm. We found that the loss of PSII activity, assayed by chlorophyll fluorescence, is faster than release of Mn from the Mn4CaO5 cluster, assayed by EPR. Loss of PSII activity and Mn release was slower during illumination in the presence of exogenous electron acceptors. Recovery of PSII activity was observed, after 30 min of addition of electron donor post illumination. The same behavior was observed under 460 and 660 nm illumination, suggesting stronger correlation between excessive excitation and photodamage compared to direct light absorption by the cluster. A unified model of PSII photodamage that takes into account present and previous literature reports is presented.Alonso ZavaferWataru KoinumaWah Soon ChowMun Hon CheahHiroyuki MinoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alonso Zavafer Wataru Koinuma Wah Soon Chow Mun Hon Cheah Hiroyuki Mino Mechanism of Photodamage of the Oxygen Evolving Mn Cluster of Photosystem II by Excessive Light Energy
description	Abstract Photodamage to Photosystem II (PSII) has been attributed either to excessive excitation of photosynthetic pigments or by direct of light absorption by Mn4CaO5 cluster. Here we investigated the time course of PSII photodamage and release of Mn in PSII-enriched membranes under high light illumination at 460 nm and 660 nm. We found that the loss of PSII activity, assayed by chlorophyll fluorescence, is faster than release of Mn from the Mn4CaO5 cluster, assayed by EPR. Loss of PSII activity and Mn release was slower during illumination in the presence of exogenous electron acceptors. Recovery of PSII activity was observed, after 30 min of addition of electron donor post illumination. The same behavior was observed under 460 and 660 nm illumination, suggesting stronger correlation between excessive excitation and photodamage compared to direct light absorption by the cluster. A unified model of PSII photodamage that takes into account present and previous literature reports is presented.
format	article
author	Alonso Zavafer Wataru Koinuma Wah Soon Chow Mun Hon Cheah Hiroyuki Mino
author_facet	Alonso Zavafer Wataru Koinuma Wah Soon Chow Mun Hon Cheah Hiroyuki Mino
author_sort	Alonso Zavafer
title	Mechanism of Photodamage of the Oxygen Evolving Mn Cluster of Photosystem II by Excessive Light Energy
title_short	Mechanism of Photodamage of the Oxygen Evolving Mn Cluster of Photosystem II by Excessive Light Energy
title_full	Mechanism of Photodamage of the Oxygen Evolving Mn Cluster of Photosystem II by Excessive Light Energy
title_fullStr	Mechanism of Photodamage of the Oxygen Evolving Mn Cluster of Photosystem II by Excessive Light Energy
title_full_unstemmed	Mechanism of Photodamage of the Oxygen Evolving Mn Cluster of Photosystem II by Excessive Light Energy
title_sort	mechanism of photodamage of the oxygen evolving mn cluster of photosystem ii by excessive light energy
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/2748f5a9514b4770af0d6004dd637a05
work_keys_str_mv	AT alonsozavafer mechanismofphotodamageoftheoxygenevolvingmnclusterofphotosystemiibyexcessivelightenergy AT watarukoinuma mechanismofphotodamageoftheoxygenevolvingmnclusterofphotosystemiibyexcessivelightenergy AT wahsoonchow mechanismofphotodamageoftheoxygenevolvingmnclusterofphotosystemiibyexcessivelightenergy AT munhoncheah mechanismofphotodamageoftheoxygenevolvingmnclusterofphotosystemiibyexcessivelightenergy AT hiroyukimino mechanismofphotodamageoftheoxygenevolvingmnclusterofphotosystemiibyexcessivelightenergy
_version_	1718388879698952192

Mechanism of Photodamage of the Oxygen Evolving Mn Cluster of Photosystem II by Excessive Light Energy

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