Two-dimensional TIRF-SIM–traction force microscopy (2D TIRF-SIM-TFM)

Quantifying rapid and small cellular forces is a major challenge in mechanobiology. Here, the authors show a >2-fold spatially and >10-fold temporally force sampling improvement combining traction force microscopy with total internal reflection fluorescence super-resolution structured illumina...

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Autores principales: Liliana Barbieri, Huw Colin-York, Kseniya Korobchevskaya, Di Li, Deanna L. Wolfson, Narain Karedla, Falk Schneider, Balpreet S. Ahluwalia, Tore Seternes, Roy A. Dalmo, Michael L. Dustin, Dong Li, Marco Fritzsche
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Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/2749e6a50094452587f5c8033adf28f0
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spelling oai:doaj.org-article:2749e6a50094452587f5c8033adf28f02021-12-02T14:30:26ZTwo-dimensional TIRF-SIM–traction force microscopy (2D TIRF-SIM-TFM)10.1038/s41467-021-22377-92041-1723https://doaj.org/article/2749e6a50094452587f5c8033adf28f02021-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-22377-9https://doaj.org/toc/2041-1723Quantifying rapid and small cellular forces is a major challenge in mechanobiology. Here, the authors show a >2-fold spatially and >10-fold temporally force sampling improvement combining traction force microscopy with total internal reflection fluorescence super-resolution structured illumination microscopy.Liliana BarbieriHuw Colin-YorkKseniya KorobchevskayaDi LiDeanna L. WolfsonNarain KaredlaFalk SchneiderBalpreet S. AhluwaliaTore SeternesRoy A. DalmoMichael L. DustinDong LiMarco FritzscheNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-14 (2021)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Liliana Barbieri
Huw Colin-York
Kseniya Korobchevskaya
Di Li
Deanna L. Wolfson
Narain Karedla
Falk Schneider
Balpreet S. Ahluwalia
Tore Seternes
Roy A. Dalmo
Michael L. Dustin
Dong Li
Marco Fritzsche
Two-dimensional TIRF-SIM–traction force microscopy (2D TIRF-SIM-TFM)
description Quantifying rapid and small cellular forces is a major challenge in mechanobiology. Here, the authors show a >2-fold spatially and >10-fold temporally force sampling improvement combining traction force microscopy with total internal reflection fluorescence super-resolution structured illumination microscopy.
format article
author Liliana Barbieri
Huw Colin-York
Kseniya Korobchevskaya
Di Li
Deanna L. Wolfson
Narain Karedla
Falk Schneider
Balpreet S. Ahluwalia
Tore Seternes
Roy A. Dalmo
Michael L. Dustin
Dong Li
Marco Fritzsche
author_facet Liliana Barbieri
Huw Colin-York
Kseniya Korobchevskaya
Di Li
Deanna L. Wolfson
Narain Karedla
Falk Schneider
Balpreet S. Ahluwalia
Tore Seternes
Roy A. Dalmo
Michael L. Dustin
Dong Li
Marco Fritzsche
author_sort Liliana Barbieri
title Two-dimensional TIRF-SIM–traction force microscopy (2D TIRF-SIM-TFM)
title_short Two-dimensional TIRF-SIM–traction force microscopy (2D TIRF-SIM-TFM)
title_full Two-dimensional TIRF-SIM–traction force microscopy (2D TIRF-SIM-TFM)
title_fullStr Two-dimensional TIRF-SIM–traction force microscopy (2D TIRF-SIM-TFM)
title_full_unstemmed Two-dimensional TIRF-SIM–traction force microscopy (2D TIRF-SIM-TFM)
title_sort two-dimensional tirf-sim–traction force microscopy (2d tirf-sim-tfm)
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/2749e6a50094452587f5c8033adf28f0
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