Integrated single- and two-photon light sheet microscopy using accelerating beams

Abstract We demonstrate the first light sheet microscope using propagation invariant, accelerating Airy beams that operates both in single- and two-photon modes. The use of the Airy beam permits us to develop an ultra compact, high resolution light sheet system without beam scanning. In two-photon m...

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Autores principales:	Peeter Piksarv, Dominik Marti, Tuan Le, Angelika Unterhuber, Lindsey H. Forbes, Melissa R. Andrews, Andreas Stingl, Wolfgang Drexler, Peter E. Andersen, Kishan Dholakia
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/274bf661225845a69183481f7c6f19ab
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spelling	oai:doaj.org-article:274bf661225845a69183481f7c6f19ab2021-12-02T16:05:58ZIntegrated single- and two-photon light sheet microscopy using accelerating beams10.1038/s41598-017-01543-42045-2322https://doaj.org/article/274bf661225845a69183481f7c6f19ab2017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01543-4https://doaj.org/toc/2045-2322Abstract We demonstrate the first light sheet microscope using propagation invariant, accelerating Airy beams that operates both in single- and two-photon modes. The use of the Airy beam permits us to develop an ultra compact, high resolution light sheet system without beam scanning. In two-photon mode, an increase in the field of view over the use of a standard Gaussian beam by a factor of six is demonstrated. This implementation for light sheet microscopy opens up new possibilities across a wide range of biomedical applications, especially for the study of neuronal processes.Peeter PiksarvDominik MartiTuan LeAngelika UnterhuberLindsey H. ForbesMelissa R. AndrewsAndreas StinglWolfgang DrexlerPeter E. AndersenKishan DholakiaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Peeter Piksarv Dominik Marti Tuan Le Angelika Unterhuber Lindsey H. Forbes Melissa R. Andrews Andreas Stingl Wolfgang Drexler Peter E. Andersen Kishan Dholakia Integrated single- and two-photon light sheet microscopy using accelerating beams
description	Abstract We demonstrate the first light sheet microscope using propagation invariant, accelerating Airy beams that operates both in single- and two-photon modes. The use of the Airy beam permits us to develop an ultra compact, high resolution light sheet system without beam scanning. In two-photon mode, an increase in the field of view over the use of a standard Gaussian beam by a factor of six is demonstrated. This implementation for light sheet microscopy opens up new possibilities across a wide range of biomedical applications, especially for the study of neuronal processes.
format	article
author	Peeter Piksarv Dominik Marti Tuan Le Angelika Unterhuber Lindsey H. Forbes Melissa R. Andrews Andreas Stingl Wolfgang Drexler Peter E. Andersen Kishan Dholakia
author_facet	Peeter Piksarv Dominik Marti Tuan Le Angelika Unterhuber Lindsey H. Forbes Melissa R. Andrews Andreas Stingl Wolfgang Drexler Peter E. Andersen Kishan Dholakia
author_sort	Peeter Piksarv
title	Integrated single- and two-photon light sheet microscopy using accelerating beams
title_short	Integrated single- and two-photon light sheet microscopy using accelerating beams
title_full	Integrated single- and two-photon light sheet microscopy using accelerating beams
title_fullStr	Integrated single- and two-photon light sheet microscopy using accelerating beams
title_full_unstemmed	Integrated single- and two-photon light sheet microscopy using accelerating beams
title_sort	integrated single- and two-photon light sheet microscopy using accelerating beams
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/274bf661225845a69183481f7c6f19ab
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Integrated single- and two-photon light sheet microscopy using accelerating beams

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