Sinonasal Stent Coated with Slow-Release Varnish of Chlorhexidine Has Sustained Protection against Bacterial Biofilm Growth in the Sinonasal Cavity: An In Vitro Study

The aim of the study was to develop a sustained-release varnish (SRV) containing chlorhexidine (CHX) for sinonasal stents (SNS) to reduce bacterial growth and biofilm formation in the sinonasal cavity. Segments of SNS were coated with SRV-CHX or SRV-placebo and exposed daily to bacterial cultures of...

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Autores principales:	Alessandra Cataldo Russomando, Ronit Vogt Sionov, Michael Friedman, Irith Gati, Ron Eliashar, Doron Steinberg, Menachem Gross
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	bacteria biofilm biofilm inhibition chlorhexidine chronic rhinosinusitis nasal stent <i>Pseudomonas aeruginosa</i> Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/275d82ae5b854ee9a0d12f2e0f99aeee
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spelling	oai:doaj.org-article:275d82ae5b854ee9a0d12f2e0f99aeee2021-11-25T18:40:39ZSinonasal Stent Coated with Slow-Release Varnish of Chlorhexidine Has Sustained Protection against Bacterial Biofilm Growth in the Sinonasal Cavity: An In Vitro Study10.3390/pharmaceutics131117831999-4923https://doaj.org/article/275d82ae5b854ee9a0d12f2e0f99aeee2021-10-01T00:00:00Zhttps://www.mdpi.com/1999-4923/13/11/1783https://doaj.org/toc/1999-4923The aim of the study was to develop a sustained-release varnish (SRV) containing chlorhexidine (CHX) for sinonasal stents (SNS) to reduce bacterial growth and biofilm formation in the sinonasal cavity. Segments of SNS were coated with SRV-CHX or SRV-placebo and exposed daily to bacterial cultures of <i>Staphylococcus aureus subsp. aureus</i> ATCC 25923 or <i>Pseudomonas aeruginosa</i> ATCC HER-1018 (PAO1). Anti-bacterial effects were assessed by disc diffusion assay and planktonic-based activity assay. Biofilm formation on the coated stents was visualized by confocal laser scanning microscopy (CLSM) and high-resolution scanning electron microscopy (HR-SEM). The metabolic activity of the biofilms was determined using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) method. Disc diffusion assay showed that SRV-CHX-coated SNS segments inhibited bacterial growth of <i>S. aureus</i><i>subsp. aureus</i> ATCC 25923 for 26 days and <i>P. aeruginosa</i> ATCC HER-1018 for 19 days. CHX was released from coated SNS segments in a pH 6 medium up to 30 days, resulting in growth inhibition of <i>S. aureus</i><i>subsp. aureus</i> ATCC 25923 for 22 days and <i>P. aeruginosa</i> ATCC HER-1018 for 24 days. The MTT assay showed a reduction of biofilm growth on the coated SNS by 69% for <i>S. aureus</i><i>subsp. aureus</i> ATCC 25923 and 40% for <i>P. aeruginosa</i> ATCC HER-1018 compared to the placebo stent after repeated exposure to planktonic growing bacteria. CLSM and HR-SEM showed a significant reduction of biofilm formation on the SRV-CHX-coated SNS segments. Coating of SNS with SRV-CHX maintains a sustained delivery of CHX, providing an inhibitory effect on the bacterial growth of <i>S. aureus</i><i>subsp. aureus</i> ATCC 25923 and <i>P. aeruginosa</i> ATCC HER-1018 for approximately 3 weeks.Alessandra Cataldo RussomandoRonit Vogt SionovMichael FriedmanIrith GatiRon EliasharDoron SteinbergMenachem GrossMDPI AGarticlebacteria biofilmbiofilm inhibitionchlorhexidinechronic rhinosinusitisnasal stent<i>Pseudomonas aeruginosa</i>Pharmacy and materia medicaRS1-441ENPharmaceutics, Vol 13, Iss 1783, p 1783 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacteria biofilm biofilm inhibition chlorhexidine chronic rhinosinusitis nasal stent <i>Pseudomonas aeruginosa</i> Pharmacy and materia medica RS1-441
spellingShingle	bacteria biofilm biofilm inhibition chlorhexidine chronic rhinosinusitis nasal stent <i>Pseudomonas aeruginosa</i> Pharmacy and materia medica RS1-441 Alessandra Cataldo Russomando Ronit Vogt Sionov Michael Friedman Irith Gati Ron Eliashar Doron Steinberg Menachem Gross Sinonasal Stent Coated with Slow-Release Varnish of Chlorhexidine Has Sustained Protection against Bacterial Biofilm Growth in the Sinonasal Cavity: An In Vitro Study
description	The aim of the study was to develop a sustained-release varnish (SRV) containing chlorhexidine (CHX) for sinonasal stents (SNS) to reduce bacterial growth and biofilm formation in the sinonasal cavity. Segments of SNS were coated with SRV-CHX or SRV-placebo and exposed daily to bacterial cultures of <i>Staphylococcus aureus subsp. aureus</i> ATCC 25923 or <i>Pseudomonas aeruginosa</i> ATCC HER-1018 (PAO1). Anti-bacterial effects were assessed by disc diffusion assay and planktonic-based activity assay. Biofilm formation on the coated stents was visualized by confocal laser scanning microscopy (CLSM) and high-resolution scanning electron microscopy (HR-SEM). The metabolic activity of the biofilms was determined using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) method. Disc diffusion assay showed that SRV-CHX-coated SNS segments inhibited bacterial growth of <i>S. aureus</i><i>subsp. aureus</i> ATCC 25923 for 26 days and <i>P. aeruginosa</i> ATCC HER-1018 for 19 days. CHX was released from coated SNS segments in a pH 6 medium up to 30 days, resulting in growth inhibition of <i>S. aureus</i><i>subsp. aureus</i> ATCC 25923 for 22 days and <i>P. aeruginosa</i> ATCC HER-1018 for 24 days. The MTT assay showed a reduction of biofilm growth on the coated SNS by 69% for <i>S. aureus</i><i>subsp. aureus</i> ATCC 25923 and 40% for <i>P. aeruginosa</i> ATCC HER-1018 compared to the placebo stent after repeated exposure to planktonic growing bacteria. CLSM and HR-SEM showed a significant reduction of biofilm formation on the SRV-CHX-coated SNS segments. Coating of SNS with SRV-CHX maintains a sustained delivery of CHX, providing an inhibitory effect on the bacterial growth of <i>S. aureus</i><i>subsp. aureus</i> ATCC 25923 and <i>P. aeruginosa</i> ATCC HER-1018 for approximately 3 weeks.
format	article
author	Alessandra Cataldo Russomando Ronit Vogt Sionov Michael Friedman Irith Gati Ron Eliashar Doron Steinberg Menachem Gross
author_facet	Alessandra Cataldo Russomando Ronit Vogt Sionov Michael Friedman Irith Gati Ron Eliashar Doron Steinberg Menachem Gross
author_sort	Alessandra Cataldo Russomando
title	Sinonasal Stent Coated with Slow-Release Varnish of Chlorhexidine Has Sustained Protection against Bacterial Biofilm Growth in the Sinonasal Cavity: An In Vitro Study
title_short	Sinonasal Stent Coated with Slow-Release Varnish of Chlorhexidine Has Sustained Protection against Bacterial Biofilm Growth in the Sinonasal Cavity: An In Vitro Study
title_full	Sinonasal Stent Coated with Slow-Release Varnish of Chlorhexidine Has Sustained Protection against Bacterial Biofilm Growth in the Sinonasal Cavity: An In Vitro Study
title_fullStr	Sinonasal Stent Coated with Slow-Release Varnish of Chlorhexidine Has Sustained Protection against Bacterial Biofilm Growth in the Sinonasal Cavity: An In Vitro Study
title_full_unstemmed	Sinonasal Stent Coated with Slow-Release Varnish of Chlorhexidine Has Sustained Protection against Bacterial Biofilm Growth in the Sinonasal Cavity: An In Vitro Study
title_sort	sinonasal stent coated with slow-release varnish of chlorhexidine has sustained protection against bacterial biofilm growth in the sinonasal cavity: an in vitro study
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/275d82ae5b854ee9a0d12f2e0f99aeee
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Sinonasal Stent Coated with Slow-Release Varnish of Chlorhexidine Has Sustained Protection against Bacterial Biofilm Growth in the Sinonasal Cavity: An In Vitro Study

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