Nanodroplet processing platform for deep and quantitative proteome profiling of 10–100 mammalian cells

There is a great need of developing highly sensitive mass spectrometry-based proteomics analysis for small cell populations. Here, the authors establish a robotically controlled chip-based nanodroplet processing platform and demonstrate its ability to profile the proteome from 10–100 mammalian cells...

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Detalles Bibliográficos
Autores principales: Ying Zhu, Paul D. Piehowski, Rui Zhao, Jing Chen, Yufeng Shen, Ronald J. Moore, Anil K. Shukla, Vladislav A. Petyuk, Martha Campbell-Thompson, Clayton E. Mathews, Richard D. Smith, Wei-Jun Qian, Ryan T. Kelly
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2018
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Acceso en línea:https://doaj.org/article/275e69e5c58341579e052e295934e528
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Sumario:There is a great need of developing highly sensitive mass spectrometry-based proteomics analysis for small cell populations. Here, the authors establish a robotically controlled chip-based nanodroplet processing platform and demonstrate its ability to profile the proteome from 10–100 mammalian cells.