IgE by itself affects mature rat mast cell preformed and de novo-synthesized mediator release and amplifies mast cell migratory response.

<h4>Background</h4>Immunoglobulin E (IgE) binds to high affinity receptor FcεRI numerously expressed on mast cells. Recent findings have revealed that IgE by itself may regulate various aspects of mast cell biology, however, detailed data is still limited.<h4>Methodology/findings&l...

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Autores principales:	Aleksandra Słodka, Magdalena Wiktorska, Ewa Brzezińska-Błaszczyk
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2761b4ec6f9744a9a4b3fbaaec0f5adf
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spelling	oai:doaj.org-article:2761b4ec6f9744a9a4b3fbaaec0f5adf2021-11-18T08:48:50ZIgE by itself affects mature rat mast cell preformed and de novo-synthesized mediator release and amplifies mast cell migratory response.1932-620310.1371/journal.pone.0079286https://doaj.org/article/2761b4ec6f9744a9a4b3fbaaec0f5adf2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24205379/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Immunoglobulin E (IgE) binds to high affinity receptor FcεRI numerously expressed on mast cells. Recent findings have revealed that IgE by itself may regulate various aspects of mast cell biology, however, detailed data is still limited.<h4>Methodology/findings</h4>Here, we have examined the influence of IgE alone, used at different concentrations, on mast cell activity and releasability. For the study we have employed in vivo differentiated mature tissue mast cells isolated from rat peritoneal cavity. Mast cells were exposed to IgE alone and then the release of preformed and de novo-synthesized mediators, surface FcεRI expression and mast cell migratory response were assessed. IgE by itself was found to up-regulate FcεRI expression and activate mast cells to degranulation, as well as de novo synthesis and release of cysteinyl leukotrienes and TNF. We have provided evidence that IgE alone also amplified spontaneous and CCL5- or TNF-induced migration of mast cells. Importantly, IgE was effective only at concentrations ≥ 3 µg/mL. A molecular basis investigation using an array of specific inhibitors showed that Src kinases, PLC/PLA2, MAP kinases (ERK and p38) and PI3K were entirely or partially involved in IgE-induced mast cell response. Furthermore, IgE alone stimulated the phosphorylation of MAP kinases and PI3K in rat mast cells.<h4>Conclusion</h4>Our results clearly demonstrated that IgE by itself, at higher concentrations, influences mast cell activity and releasability. As there are different conditions when the IgE level is raised it might be supposed that in vivo IgE is one of the important factors modulating mast cell biology within tissues.Aleksandra SłodkaMagdalena WiktorskaEwa Brzezińska-BłaszczykPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 10, p e79286 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aleksandra Słodka Magdalena Wiktorska Ewa Brzezińska-Błaszczyk IgE by itself affects mature rat mast cell preformed and de novo-synthesized mediator release and amplifies mast cell migratory response.
description	<h4>Background</h4>Immunoglobulin E (IgE) binds to high affinity receptor FcεRI numerously expressed on mast cells. Recent findings have revealed that IgE by itself may regulate various aspects of mast cell biology, however, detailed data is still limited.<h4>Methodology/findings</h4>Here, we have examined the influence of IgE alone, used at different concentrations, on mast cell activity and releasability. For the study we have employed in vivo differentiated mature tissue mast cells isolated from rat peritoneal cavity. Mast cells were exposed to IgE alone and then the release of preformed and de novo-synthesized mediators, surface FcεRI expression and mast cell migratory response were assessed. IgE by itself was found to up-regulate FcεRI expression and activate mast cells to degranulation, as well as de novo synthesis and release of cysteinyl leukotrienes and TNF. We have provided evidence that IgE alone also amplified spontaneous and CCL5- or TNF-induced migration of mast cells. Importantly, IgE was effective only at concentrations ≥ 3 µg/mL. A molecular basis investigation using an array of specific inhibitors showed that Src kinases, PLC/PLA2, MAP kinases (ERK and p38) and PI3K were entirely or partially involved in IgE-induced mast cell response. Furthermore, IgE alone stimulated the phosphorylation of MAP kinases and PI3K in rat mast cells.<h4>Conclusion</h4>Our results clearly demonstrated that IgE by itself, at higher concentrations, influences mast cell activity and releasability. As there are different conditions when the IgE level is raised it might be supposed that in vivo IgE is one of the important factors modulating mast cell biology within tissues.
format	article
author	Aleksandra Słodka Magdalena Wiktorska Ewa Brzezińska-Błaszczyk
author_facet	Aleksandra Słodka Magdalena Wiktorska Ewa Brzezińska-Błaszczyk
author_sort	Aleksandra Słodka
title	IgE by itself affects mature rat mast cell preformed and de novo-synthesized mediator release and amplifies mast cell migratory response.
title_short	IgE by itself affects mature rat mast cell preformed and de novo-synthesized mediator release and amplifies mast cell migratory response.
title_full	IgE by itself affects mature rat mast cell preformed and de novo-synthesized mediator release and amplifies mast cell migratory response.
title_fullStr	IgE by itself affects mature rat mast cell preformed and de novo-synthesized mediator release and amplifies mast cell migratory response.
title_full_unstemmed	IgE by itself affects mature rat mast cell preformed and de novo-synthesized mediator release and amplifies mast cell migratory response.
title_sort	ige by itself affects mature rat mast cell preformed and de novo-synthesized mediator release and amplifies mast cell migratory response.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/2761b4ec6f9744a9a4b3fbaaec0f5adf
work_keys_str_mv	AT aleksandrasłodka igebyitselfaffectsmatureratmastcellpreformedanddenovosynthesizedmediatorreleaseandamplifiesmastcellmigratoryresponse AT magdalenawiktorska igebyitselfaffectsmatureratmastcellpreformedanddenovosynthesizedmediatorreleaseandamplifiesmastcellmigratoryresponse AT ewabrzezinskabłaszczyk igebyitselfaffectsmatureratmastcellpreformedanddenovosynthesizedmediatorreleaseandamplifiesmastcellmigratoryresponse
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IgE by itself affects mature rat mast cell preformed and de novo-synthesized mediator release and amplifies mast cell migratory response.

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