Tag-seq: a convenient and scalable method for genome-wide specificity assessment of CRISPR/Cas nucleases

Hongxin Huang and Yongfei Hu et al. develop Tag-seq, a streamlined sequencing method to examine off-target editing by CRISPR-Cas systems. The authors validate Tag-seq in multiple cell lines, demonstrating its broad utility and flexibility in identifying off-target genome editing.

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Autores principales:	Hongxin Huang, Yongfei Hu, Guanjie Huang, Shufeng Ma, Jianqi Feng, Dong Wang, Ying Lin, Jiajian Zhou, Zhili Rong
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/276463593c4b463886879126e4ba92c9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

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spelling	oai:doaj.org-article:276463593c4b463886879126e4ba92c92021-12-02T16:10:32ZTag-seq: a convenient and scalable method for genome-wide specificity assessment of CRISPR/Cas nucleases10.1038/s42003-021-02351-32399-3642https://doaj.org/article/276463593c4b463886879126e4ba92c92021-07-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02351-3https://doaj.org/toc/2399-3642Hongxin Huang and Yongfei Hu et al. develop Tag-seq, a streamlined sequencing method to examine off-target editing by CRISPR-Cas systems. The authors validate Tag-seq in multiple cell lines, demonstrating its broad utility and flexibility in identifying off-target genome editing.Hongxin HuangYongfei HuGuanjie HuangShufeng MaJianqi FengDong WangYing LinJiajian ZhouZhili RongNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Hongxin Huang Yongfei Hu Guanjie Huang Shufeng Ma Jianqi Feng Dong Wang Ying Lin Jiajian Zhou Zhili Rong Tag-seq: a convenient and scalable method for genome-wide specificity assessment of CRISPR/Cas nucleases
description	Hongxin Huang and Yongfei Hu et al. develop Tag-seq, a streamlined sequencing method to examine off-target editing by CRISPR-Cas systems. The authors validate Tag-seq in multiple cell lines, demonstrating its broad utility and flexibility in identifying off-target genome editing.
format	article
author	Hongxin Huang Yongfei Hu Guanjie Huang Shufeng Ma Jianqi Feng Dong Wang Ying Lin Jiajian Zhou Zhili Rong
author_facet	Hongxin Huang Yongfei Hu Guanjie Huang Shufeng Ma Jianqi Feng Dong Wang Ying Lin Jiajian Zhou Zhili Rong
author_sort	Hongxin Huang
title	Tag-seq: a convenient and scalable method for genome-wide specificity assessment of CRISPR/Cas nucleases
title_short	Tag-seq: a convenient and scalable method for genome-wide specificity assessment of CRISPR/Cas nucleases
title_full	Tag-seq: a convenient and scalable method for genome-wide specificity assessment of CRISPR/Cas nucleases
title_fullStr	Tag-seq: a convenient and scalable method for genome-wide specificity assessment of CRISPR/Cas nucleases
title_full_unstemmed	Tag-seq: a convenient and scalable method for genome-wide specificity assessment of CRISPR/Cas nucleases
title_sort	tag-seq: a convenient and scalable method for genome-wide specificity assessment of crispr/cas nucleases
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/276463593c4b463886879126e4ba92c9
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Tag-seq: a convenient and scalable method for genome-wide specificity assessment of CRISPR/Cas nucleases

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