Tag-seq: a convenient and scalable method for genome-wide specificity assessment of CRISPR/Cas nucleases

Hongxin Huang and Yongfei Hu et al. develop Tag-seq, a streamlined sequencing method to examine off-target editing by CRISPR-Cas systems. The authors validate Tag-seq in multiple cell lines, demonstrating its broad utility and flexibility in identifying off-target genome editing.

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Autores principales: Hongxin Huang, Yongfei Hu, Guanjie Huang, Shufeng Ma, Jianqi Feng, Dong Wang, Ying Lin, Jiajian Zhou, Zhili Rong
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/276463593c4b463886879126e4ba92c9
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