Intracellular SERS monitoring of drug release from plasmonic-assisted biosilica nanoparticles

Nanoscale delivery systems have been investigated for therapy due to their advantages, including the sustained delivery of drugs to cells and reduction of systemic toxicity compared to conventional treatments. However, their application is still hampered by experimental challenges, such as the inves...

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Autores principales:	Tramontano Chiara, Managò Stefano, Delle Cave Donatella, Chianese Giovanna, Lonardo Enza, De Stefano Luca, De Luca Anna Chiara, Rea Ilaria
Formato:	article
Lenguaje:	EN
Publicado:	EDP Sciences 2021
Materias:	Physics QC1-999
Acceso en línea:	https://doaj.org/article/276cbf58f7144ab3a428838ad083af73
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spelling	oai:doaj.org-article:276cbf58f7144ab3a428838ad083af732021-12-02T17:12:51ZIntracellular SERS monitoring of drug release from plasmonic-assisted biosilica nanoparticles2100-014X10.1051/epjconf/202125513002https://doaj.org/article/276cbf58f7144ab3a428838ad083af732021-01-01T00:00:00Zhttps://www.epj-conferences.org/articles/epjconf/pdf/2021/09/epjconf_eosam2021_13002.pdfhttps://doaj.org/toc/2100-014XNanoscale delivery systems have been investigated for therapy due to their advantages, including the sustained delivery of drugs to cells and reduction of systemic toxicity compared to conventional treatments. However, their application is still hampered by experimental challenges, such as the investigation of the drug release in cells rather than in vitro. Here, we describe a hybrid nanoplatform for monitoring the drug release in living colorectal cancer (CRC) cells by Surface-Enhanced Raman Scattering (SERS). Specifically, the anticancer drug Galunisertib is encapsulated in diatomite nanoparticles (DNPs) decorated by gold nanoparticles (AuNPs) and capped by gelatin. The combination of DNP loading capacities with the Raman enhancement of Galunisertib provided by AuNPs enables bio-imaging and drug delivery without using fluorophores or markers, avoiding fluorescence-quenching issues. Thanks to the Raman enhancement of Galunisertib, the drug release profile is monitored and quantified in living cells by SERS with a femtogram scale resolution. When the gelatin shell is digested by proteases, Galunisertib is released and its SERS spectrum decreases, allowing real-time quantification in CRC cells. The therapeutic efficiency of the Galunisertib delivery platform offers an alternative route for lowering drug dose and toxicity.Tramontano ChiaraManagò StefanoDelle Cave DonatellaChianese GiovannaLonardo EnzaDe Stefano LucaDe Luca Anna ChiaraRea IlariaEDP SciencesarticlePhysicsQC1-999ENEPJ Web of Conferences, Vol 255, p 13002 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Physics QC1-999
spellingShingle	Physics QC1-999 Tramontano Chiara Managò Stefano Delle Cave Donatella Chianese Giovanna Lonardo Enza De Stefano Luca De Luca Anna Chiara Rea Ilaria Intracellular SERS monitoring of drug release from plasmonic-assisted biosilica nanoparticles
description	Nanoscale delivery systems have been investigated for therapy due to their advantages, including the sustained delivery of drugs to cells and reduction of systemic toxicity compared to conventional treatments. However, their application is still hampered by experimental challenges, such as the investigation of the drug release in cells rather than in vitro. Here, we describe a hybrid nanoplatform for monitoring the drug release in living colorectal cancer (CRC) cells by Surface-Enhanced Raman Scattering (SERS). Specifically, the anticancer drug Galunisertib is encapsulated in diatomite nanoparticles (DNPs) decorated by gold nanoparticles (AuNPs) and capped by gelatin. The combination of DNP loading capacities with the Raman enhancement of Galunisertib provided by AuNPs enables bio-imaging and drug delivery without using fluorophores or markers, avoiding fluorescence-quenching issues. Thanks to the Raman enhancement of Galunisertib, the drug release profile is monitored and quantified in living cells by SERS with a femtogram scale resolution. When the gelatin shell is digested by proteases, Galunisertib is released and its SERS spectrum decreases, allowing real-time quantification in CRC cells. The therapeutic efficiency of the Galunisertib delivery platform offers an alternative route for lowering drug dose and toxicity.
format	article
author	Tramontano Chiara Managò Stefano Delle Cave Donatella Chianese Giovanna Lonardo Enza De Stefano Luca De Luca Anna Chiara Rea Ilaria
author_facet	Tramontano Chiara Managò Stefano Delle Cave Donatella Chianese Giovanna Lonardo Enza De Stefano Luca De Luca Anna Chiara Rea Ilaria
author_sort	Tramontano Chiara
title	Intracellular SERS monitoring of drug release from plasmonic-assisted biosilica nanoparticles
title_short	Intracellular SERS monitoring of drug release from plasmonic-assisted biosilica nanoparticles
title_full	Intracellular SERS monitoring of drug release from plasmonic-assisted biosilica nanoparticles
title_fullStr	Intracellular SERS monitoring of drug release from plasmonic-assisted biosilica nanoparticles
title_full_unstemmed	Intracellular SERS monitoring of drug release from plasmonic-assisted biosilica nanoparticles
title_sort	intracellular sers monitoring of drug release from plasmonic-assisted biosilica nanoparticles
publisher	EDP Sciences
publishDate	2021
url	https://doaj.org/article/276cbf58f7144ab3a428838ad083af73
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Intracellular SERS monitoring of drug release from plasmonic-assisted biosilica nanoparticles

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