Use of Blue Agar CAS Assay for Siderophore Detection

Schwyn and Neiland developed a universal siderophore assay using chrome azurol S(CAS) and hexadecyltrimethylammonium bromide (HDTMA) as indicators. In Schwyn and Neiland’s original paper, the procedure given for making CAS agar is written in general terms and can be difficult to follow, especially f...

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Autores principales:	Brian C. Louden, Daniel Haarmann, Aaron M. Lynne
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2011
Materias:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/277285351232488eaf3783290d6be43a
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spelling	oai:doaj.org-article:277285351232488eaf3783290d6be43a2021-11-15T15:04:10ZUse of Blue Agar CAS Assay for Siderophore Detection10.1128/jmbe.v12i1.2491935-78851935-7877https://doaj.org/article/277285351232488eaf3783290d6be43a2011-01-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v12i1.249https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885Schwyn and Neiland developed a universal siderophore assay using chrome azurol S(CAS) and hexadecyltrimethylammonium bromide (HDTMA) as indicators. In Schwyn and Neiland’s original paper, the procedure given for making CAS agar is written in general terms and can be difficult to follow, especially for an individual who has limited experience making more complex media. Here, we give a step-by-step protocol for making the CAS agar plates, and we discuss how this media can be incorporated in a comprehensive project in a microbiology lab course for biology majors.Brian C. LoudenDaniel HaarmannAaron M. LynneAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 12, Iss 1, Pp 51-53 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 Brian C. Louden Daniel Haarmann Aaron M. Lynne Use of Blue Agar CAS Assay for Siderophore Detection
description	Schwyn and Neiland developed a universal siderophore assay using chrome azurol S(CAS) and hexadecyltrimethylammonium bromide (HDTMA) as indicators. In Schwyn and Neiland’s original paper, the procedure given for making CAS agar is written in general terms and can be difficult to follow, especially for an individual who has limited experience making more complex media. Here, we give a step-by-step protocol for making the CAS agar plates, and we discuss how this media can be incorporated in a comprehensive project in a microbiology lab course for biology majors.
format	article
author	Brian C. Louden Daniel Haarmann Aaron M. Lynne
author_facet	Brian C. Louden Daniel Haarmann Aaron M. Lynne
author_sort	Brian C. Louden
title	Use of Blue Agar CAS Assay for Siderophore Detection
title_short	Use of Blue Agar CAS Assay for Siderophore Detection
title_full	Use of Blue Agar CAS Assay for Siderophore Detection
title_fullStr	Use of Blue Agar CAS Assay for Siderophore Detection
title_full_unstemmed	Use of Blue Agar CAS Assay for Siderophore Detection
title_sort	use of blue agar cas assay for siderophore detection
publisher	American Society for Microbiology
publishDate	2011
url	https://doaj.org/article/277285351232488eaf3783290d6be43a
work_keys_str_mv	AT brianclouden useofblueagarcasassayforsiderophoredetection AT danielhaarmann useofblueagarcasassayforsiderophoredetection AT aaronmlynne useofblueagarcasassayforsiderophoredetection
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Use of Blue Agar CAS Assay for Siderophore Detection

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