Not All Lectins Are Equally Suitable for Labeling Rodent Vasculature

The vascular system is vital for all tissues and the interest in its visualization spans many fields. A number of different plant-derived lectins are used for detection of vasculature; however, studies performing direct comparison of the labeling efficacy of different lectins and techniques are lack...

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Autores principales:	Roberta Battistella, Marios Kritsilis, Hana Matuskova, Douglas Haswell, Anne Xiaoan Cheng, Anja Meissner, Maiken Nedergaard, Iben Lundgaard
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	lectins lectin angiography blood vessels vascular research stroke angiogenesis Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/2775e13ac7734fd68eea1e35fd93b552
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spelling	oai:doaj.org-article:2775e13ac7734fd68eea1e35fd93b5522021-11-11T17:02:06ZNot All Lectins Are Equally Suitable for Labeling Rodent Vasculature10.3390/ijms2221115541422-00671661-6596https://doaj.org/article/2775e13ac7734fd68eea1e35fd93b5522021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11554https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067The vascular system is vital for all tissues and the interest in its visualization spans many fields. A number of different plant-derived lectins are used for detection of vasculature; however, studies performing direct comparison of the labeling efficacy of different lectins and techniques are lacking. In this study, we compared the labeling efficacy of three lectins: <i>Griffonia simplicifolia</i> isolectin B4 (IB4); <i>wheat germ agglutinin</i> (WGA), and <i>Lycopersicon esculentum agglutinin</i> (LEA). The LEA lectin was identified as being far superior to the IB4 and WGA lectins in histological labeling of blood vessels in brain sections. A similar signal-to-noise ratio was achieved with high concentrations of the WGA lectin injected during intracardial perfusion. Lectins were also suitable for labeling vasculature in other tissues, including spinal cord, dura mater, heart, skeletal muscle, kidney, and liver tissues. In uninjured tissues, the LEA lectin was as accurate as the Tie2–eGFP reporter mice and GLUT-1 immunohistochemistry for labeling the cerebral vasculature, validating its specificity and sensitivity. However, in pathological situations, e.g., in stroke, the sensitivity of the LEA lectin decreases dramatically, limiting its applicability in such studies. This work can be used for selecting the type of lectin and labeling method for various tissues.Roberta BattistellaMarios KritsilisHana MatuskovaDouglas HaswellAnne Xiaoan ChengAnja MeissnerMaiken NedergaardIben LundgaardMDPI AGarticlelectinslectin angiographyblood vesselsvascular researchstrokeangiogenesisBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11554, p 11554 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	lectins lectin angiography blood vessels vascular research stroke angiogenesis Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	lectins lectin angiography blood vessels vascular research stroke angiogenesis Biology (General) QH301-705.5 Chemistry QD1-999 Roberta Battistella Marios Kritsilis Hana Matuskova Douglas Haswell Anne Xiaoan Cheng Anja Meissner Maiken Nedergaard Iben Lundgaard Not All Lectins Are Equally Suitable for Labeling Rodent Vasculature
description	The vascular system is vital for all tissues and the interest in its visualization spans many fields. A number of different plant-derived lectins are used for detection of vasculature; however, studies performing direct comparison of the labeling efficacy of different lectins and techniques are lacking. In this study, we compared the labeling efficacy of three lectins: <i>Griffonia simplicifolia</i> isolectin B4 (IB4); <i>wheat germ agglutinin</i> (WGA), and <i>Lycopersicon esculentum agglutinin</i> (LEA). The LEA lectin was identified as being far superior to the IB4 and WGA lectins in histological labeling of blood vessels in brain sections. A similar signal-to-noise ratio was achieved with high concentrations of the WGA lectin injected during intracardial perfusion. Lectins were also suitable for labeling vasculature in other tissues, including spinal cord, dura mater, heart, skeletal muscle, kidney, and liver tissues. In uninjured tissues, the LEA lectin was as accurate as the Tie2–eGFP reporter mice and GLUT-1 immunohistochemistry for labeling the cerebral vasculature, validating its specificity and sensitivity. However, in pathological situations, e.g., in stroke, the sensitivity of the LEA lectin decreases dramatically, limiting its applicability in such studies. This work can be used for selecting the type of lectin and labeling method for various tissues.
format	article
author	Roberta Battistella Marios Kritsilis Hana Matuskova Douglas Haswell Anne Xiaoan Cheng Anja Meissner Maiken Nedergaard Iben Lundgaard
author_facet	Roberta Battistella Marios Kritsilis Hana Matuskova Douglas Haswell Anne Xiaoan Cheng Anja Meissner Maiken Nedergaard Iben Lundgaard
author_sort	Roberta Battistella
title	Not All Lectins Are Equally Suitable for Labeling Rodent Vasculature
title_short	Not All Lectins Are Equally Suitable for Labeling Rodent Vasculature
title_full	Not All Lectins Are Equally Suitable for Labeling Rodent Vasculature
title_fullStr	Not All Lectins Are Equally Suitable for Labeling Rodent Vasculature
title_full_unstemmed	Not All Lectins Are Equally Suitable for Labeling Rodent Vasculature
title_sort	not all lectins are equally suitable for labeling rodent vasculature
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2775e13ac7734fd68eea1e35fd93b552
work_keys_str_mv	AT robertabattistella notalllectinsareequallysuitableforlabelingrodentvasculature AT marioskritsilis notalllectinsareequallysuitableforlabelingrodentvasculature AT hanamatuskova notalllectinsareequallysuitableforlabelingrodentvasculature AT douglashaswell notalllectinsareequallysuitableforlabelingrodentvasculature AT annexiaoancheng notalllectinsareequallysuitableforlabelingrodentvasculature AT anjameissner notalllectinsareequallysuitableforlabelingrodentvasculature AT maikennedergaard notalllectinsareequallysuitableforlabelingrodentvasculature AT ibenlundgaard notalllectinsareequallysuitableforlabelingrodentvasculature
_version_	1718432165814861824

Not All Lectins Are Equally Suitable for Labeling Rodent Vasculature

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