Proteomics of <i>Cryptococcus neoformans</i>: From the Lab to the Clinic

Fungal pathogens cause an array of diseases by targeting both immunocompromised and immunocompetent hosts. Fungi overcome our current arsenal of antifungals through the emergence and evolution of resistance. In particular, the human fungal pathogen, <i>Cryptococcus neoformans</i> is foun...

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Autores principales:	Ben Muselius, Shay-Lynn Durand, Jennifer Geddes-McAlister
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Cryptococcus neoformans</i> mass spectrometry-based proteomics fungal pathogenesis in vitro and in vivo models personalized medicine Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/27779c36e7b24c5a8e4b390eeb49ba93
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spelling	oai:doaj.org-article:27779c36e7b24c5a8e4b390eeb49ba932021-11-25T17:56:11ZProteomics of <i>Cryptococcus neoformans</i>: From the Lab to the Clinic10.3390/ijms2222123901422-00671661-6596https://doaj.org/article/27779c36e7b24c5a8e4b390eeb49ba932021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12390https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Fungal pathogens cause an array of diseases by targeting both immunocompromised and immunocompetent hosts. Fungi overcome our current arsenal of antifungals through the emergence and evolution of resistance. In particular, the human fungal pathogen, <i>Cryptococcus neoformans</i> is found ubiquitously within the environment and causes severe disease in immunocompromised individuals around the globe with limited treatment options available. To uncover fundamental knowledge about this fungal pathogen, as well as investigate new detection and treatment strategies, mass spectrometry-based proteomics provides a plethora of tools and applications, as well as bioinformatics platforms. In this review, we highlight proteomics approaches within the laboratory to investigate changes in the cellular proteome, secretome, and extracellular vesicles. We also explore regulation by post-translational modifications and the impact of protein–protein interactions. Further, we present the development and comprehensive assessment of murine models of cryptococcal infection, which provide valuable tools to define the dynamic relationship between the host and pathogen during disease. Finally, we explore recent quantitative proteomics studies that begin to extrapolate the findings from the bench to the clinic for improved methods of fungal detection and monitoring. Such studies support a framework for personalized medical approaches to eradicate diseases caused by <i>C. neoformans</i>.Ben MuseliusShay-Lynn DurandJennifer Geddes-McAlisterMDPI AGarticle<i>Cryptococcus neoformans</i>mass spectrometry-based proteomicsfungal pathogenesisin vitro and in vivo modelspersonalized medicineBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12390, p 12390 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Cryptococcus neoformans</i> mass spectrometry-based proteomics fungal pathogenesis in vitro and in vivo models personalized medicine Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	<i>Cryptococcus neoformans</i> mass spectrometry-based proteomics fungal pathogenesis in vitro and in vivo models personalized medicine Biology (General) QH301-705.5 Chemistry QD1-999 Ben Muselius Shay-Lynn Durand Jennifer Geddes-McAlister Proteomics of <i>Cryptococcus neoformans</i>: From the Lab to the Clinic
description	Fungal pathogens cause an array of diseases by targeting both immunocompromised and immunocompetent hosts. Fungi overcome our current arsenal of antifungals through the emergence and evolution of resistance. In particular, the human fungal pathogen, <i>Cryptococcus neoformans</i> is found ubiquitously within the environment and causes severe disease in immunocompromised individuals around the globe with limited treatment options available. To uncover fundamental knowledge about this fungal pathogen, as well as investigate new detection and treatment strategies, mass spectrometry-based proteomics provides a plethora of tools and applications, as well as bioinformatics platforms. In this review, we highlight proteomics approaches within the laboratory to investigate changes in the cellular proteome, secretome, and extracellular vesicles. We also explore regulation by post-translational modifications and the impact of protein–protein interactions. Further, we present the development and comprehensive assessment of murine models of cryptococcal infection, which provide valuable tools to define the dynamic relationship between the host and pathogen during disease. Finally, we explore recent quantitative proteomics studies that begin to extrapolate the findings from the bench to the clinic for improved methods of fungal detection and monitoring. Such studies support a framework for personalized medical approaches to eradicate diseases caused by <i>C. neoformans</i>.
format	article
author	Ben Muselius Shay-Lynn Durand Jennifer Geddes-McAlister
author_facet	Ben Muselius Shay-Lynn Durand Jennifer Geddes-McAlister
author_sort	Ben Muselius
title	Proteomics of <i>Cryptococcus neoformans</i>: From the Lab to the Clinic
title_short	Proteomics of <i>Cryptococcus neoformans</i>: From the Lab to the Clinic
title_full	Proteomics of <i>Cryptococcus neoformans</i>: From the Lab to the Clinic
title_fullStr	Proteomics of <i>Cryptococcus neoformans</i>: From the Lab to the Clinic
title_full_unstemmed	Proteomics of <i>Cryptococcus neoformans</i>: From the Lab to the Clinic
title_sort	proteomics of <i>cryptococcus neoformans</i>: from the lab to the clinic
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/27779c36e7b24c5a8e4b390eeb49ba93
work_keys_str_mv	AT benmuselius proteomicsoficryptococcusneoformansifromthelabtotheclinic AT shaylynndurand proteomicsoficryptococcusneoformansifromthelabtotheclinic AT jennifergeddesmcalister proteomicsoficryptococcusneoformansifromthelabtotheclinic
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Proteomics of <i>Cryptococcus neoformans</i>: From the Lab to the Clinic

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