Devil's Claw to suppress appetite--ghrelin receptor modulation potential of a Harpagophytum procumbens root extract.

Ghrelin is a stomach-derived peptide that has been identified as the only circulating hunger hormone that exerts a potent orexigenic effect via activation of its receptor, the growth hormone secretagogue receptor (GHS-R1a). Hence, the ghrelinergic system represents a promising target to treat obesit...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Cristina Torres-Fuentes, Wessel F Theeuwes, Michael K McMullen, Anna K McMullen, Timothy G Dinan, John F Cryan, Harriët Schellekens
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/27785b5c2f0846ad866ac6a5163f1a90
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:27785b5c2f0846ad866ac6a5163f1a90
record_format	dspace
spelling	oai:doaj.org-article:27785b5c2f0846ad866ac6a5163f1a902021-11-25T06:06:54ZDevil's Claw to suppress appetite--ghrelin receptor modulation potential of a Harpagophytum procumbens root extract.1932-620310.1371/journal.pone.0103118https://doaj.org/article/27785b5c2f0846ad866ac6a5163f1a902014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25068823/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Ghrelin is a stomach-derived peptide that has been identified as the only circulating hunger hormone that exerts a potent orexigenic effect via activation of its receptor, the growth hormone secretagogue receptor (GHS-R1a). Hence, the ghrelinergic system represents a promising target to treat obesity and obesity-related diseases. In this study we analysed the GHS-R1a receptor activating potential of Harpagophytum procumbens, popularly known as Devil's Claw, and its effect on food intake in vivo. H. procumbens is an important traditional medicinal plant from Southern Africa with potent anti-inflammatory and analgesic effects. This plant has been also used as an appetite modulator but most evidences are anecdotal and to our knowledge, no clear scientific studies relating to appetite modulation have been done to this date. The ghrelin receptor activation potential of an extract derived from the dried tuberous roots of H. procumbens was analysed by calcium mobilization and receptor internalization assays in human embryonic kidney cells (Hek) stably expressing the GHS-R1a receptor. Food intake was investigated in male C57BL/6 mice following intraperitoneal administration of H. procumbens root extract in ad libitum and food restricted conditions. Exposure to H. procumbens extract demonstrated a significant increased cellular calcium influx but did not induce subsequent GHS-R1a receptor internalization, which is a characteristic for full receptor activation. A significant anorexigenic effect was observed in male C57BL/6 mice following peripheral administration of H. procumbens extract. We conclude that H. procumbens root extract is a potential novel source for potent anti-obesity bioactives. These results reinforce the promising potential of natural bioactives to be developed into functional foods with weight-loss and weight maintenance benefits.Cristina Torres-FuentesWessel F TheeuwesMichael K McMullenAnna K McMullenTimothy G DinanJohn F CryanHarriët SchellekensPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 7, p e103118 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Cristina Torres-Fuentes Wessel F Theeuwes Michael K McMullen Anna K McMullen Timothy G Dinan John F Cryan Harriët Schellekens Devil's Claw to suppress appetite--ghrelin receptor modulation potential of a Harpagophytum procumbens root extract.
description	Ghrelin is a stomach-derived peptide that has been identified as the only circulating hunger hormone that exerts a potent orexigenic effect via activation of its receptor, the growth hormone secretagogue receptor (GHS-R1a). Hence, the ghrelinergic system represents a promising target to treat obesity and obesity-related diseases. In this study we analysed the GHS-R1a receptor activating potential of Harpagophytum procumbens, popularly known as Devil's Claw, and its effect on food intake in vivo. H. procumbens is an important traditional medicinal plant from Southern Africa with potent anti-inflammatory and analgesic effects. This plant has been also used as an appetite modulator but most evidences are anecdotal and to our knowledge, no clear scientific studies relating to appetite modulation have been done to this date. The ghrelin receptor activation potential of an extract derived from the dried tuberous roots of H. procumbens was analysed by calcium mobilization and receptor internalization assays in human embryonic kidney cells (Hek) stably expressing the GHS-R1a receptor. Food intake was investigated in male C57BL/6 mice following intraperitoneal administration of H. procumbens root extract in ad libitum and food restricted conditions. Exposure to H. procumbens extract demonstrated a significant increased cellular calcium influx but did not induce subsequent GHS-R1a receptor internalization, which is a characteristic for full receptor activation. A significant anorexigenic effect was observed in male C57BL/6 mice following peripheral administration of H. procumbens extract. We conclude that H. procumbens root extract is a potential novel source for potent anti-obesity bioactives. These results reinforce the promising potential of natural bioactives to be developed into functional foods with weight-loss and weight maintenance benefits.
format	article
author	Cristina Torres-Fuentes Wessel F Theeuwes Michael K McMullen Anna K McMullen Timothy G Dinan John F Cryan Harriët Schellekens
author_facet	Cristina Torres-Fuentes Wessel F Theeuwes Michael K McMullen Anna K McMullen Timothy G Dinan John F Cryan Harriët Schellekens
author_sort	Cristina Torres-Fuentes
title	Devil's Claw to suppress appetite--ghrelin receptor modulation potential of a Harpagophytum procumbens root extract.
title_short	Devil's Claw to suppress appetite--ghrelin receptor modulation potential of a Harpagophytum procumbens root extract.
title_full	Devil's Claw to suppress appetite--ghrelin receptor modulation potential of a Harpagophytum procumbens root extract.
title_fullStr	Devil's Claw to suppress appetite--ghrelin receptor modulation potential of a Harpagophytum procumbens root extract.
title_full_unstemmed	Devil's Claw to suppress appetite--ghrelin receptor modulation potential of a Harpagophytum procumbens root extract.
title_sort	devil's claw to suppress appetite--ghrelin receptor modulation potential of a harpagophytum procumbens root extract.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/27785b5c2f0846ad866ac6a5163f1a90
work_keys_str_mv	AT cristinatorresfuentes devilsclawtosuppressappetiteghrelinreceptormodulationpotentialofaharpagophytumprocumbensrootextract AT wesselftheeuwes devilsclawtosuppressappetiteghrelinreceptormodulationpotentialofaharpagophytumprocumbensrootextract AT michaelkmcmullen devilsclawtosuppressappetiteghrelinreceptormodulationpotentialofaharpagophytumprocumbensrootextract AT annakmcmullen devilsclawtosuppressappetiteghrelinreceptormodulationpotentialofaharpagophytumprocumbensrootextract AT timothygdinan devilsclawtosuppressappetiteghrelinreceptormodulationpotentialofaharpagophytumprocumbensrootextract AT johnfcryan devilsclawtosuppressappetiteghrelinreceptormodulationpotentialofaharpagophytumprocumbensrootextract AT harrietschellekens devilsclawtosuppressappetiteghrelinreceptormodulationpotentialofaharpagophytumprocumbensrootextract
_version_	1718414152195637248

Devil's Claw to suppress appetite--ghrelin receptor modulation potential of a Harpagophytum procumbens root extract.

Ejemplares similares