Antiparasitic potential of Nephrolepis biserrata methanol extract against the parasitic leech Zeylanicobdella arugamensis (Hirudinea) and LC-QTOF analysis

Abstract Marine leech Zeylanicobdella arugamensis (Piscicolidae), an economically important parasite is infesting predominantly cultured groupers, hybrid groupers and other fish in Southeast Asian countries. In this study, we tested the anti-parasitic potential of a medicinal plant Nephrolepis biser...

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Autores principales:	Muhammad Dawood Shah, Balu Alagar Venmathi Maran, Fatin Khairah Haron, Julian Ransangan, Fui Fui Ching, Sitti Raehanah Muhamad Shaleh, Rossita Shapawi, Yoong Soon Yong, Susumu Ohtsuka
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/277a71bd0fc045ac8d6ea2181ceeae3a
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spelling	oai:doaj.org-article:277a71bd0fc045ac8d6ea2181ceeae3a2021-12-02T12:40:41ZAntiparasitic potential of Nephrolepis biserrata methanol extract against the parasitic leech Zeylanicobdella arugamensis (Hirudinea) and LC-QTOF analysis10.1038/s41598-020-79094-42045-2322https://doaj.org/article/277a71bd0fc045ac8d6ea2181ceeae3a2020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79094-4https://doaj.org/toc/2045-2322Abstract Marine leech Zeylanicobdella arugamensis (Piscicolidae), an economically important parasite is infesting predominantly cultured groupers, hybrid groupers and other fish in Southeast Asian countries. In this study, we tested the anti-parasitic potential of a medicinal plant Nephrolepis biserrata found in Sabah, East Malaysia against Z. arugamensis. Various concentrations of methanol extracts of the plant were tested experimentally against Z. arugamensis and disinfestation of the leech from its primary host hybrid groupers. The composition of methanol extract of N. biserrata was determined through LC-QTOF analysis. The significant anti-parasitic activity of 100% mortality of leeches was observed with the exposure of N. biserrata extracts. The average time to kill the leeches at concentrations of 25, 50 and 100 mg/ml was 25.11 ± 3.26, 11.91 ± 0.99, and 4.88 ± 0.50 min., respectively. Further, at various low concentrations of N. biserrata 2.5, 5 and 10 mg/ml, hybrid groupers were disinfested in an average time of 108.33 ± 12.65, 65.83 ± 9.70 and 29.16 ± 5.85 min., respectively. The tandem mass spectrometry data from LC-QTOF indicated some hits on useful bioactive compounds such as terpenoids (ivalin, isovelleral, brassinolide, and eschscholtzxanthin), flavonoids (alnustin, kaempferol 7,4′-dimethyl ether, and pachypodol), phenolics (piscidic acid, chlorogenic acid, and ankorine), and aromatic (3-hydroxycoumarin). Thus N. biserrata can act as a potential biocontrol agent.Muhammad Dawood ShahBalu Alagar Venmathi MaranFatin Khairah HaronJulian RansanganFui Fui ChingSitti Raehanah Muhamad ShalehRossita ShapawiYoong Soon YongSusumu OhtsukaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Muhammad Dawood Shah Balu Alagar Venmathi Maran Fatin Khairah Haron Julian Ransangan Fui Fui Ching Sitti Raehanah Muhamad Shaleh Rossita Shapawi Yoong Soon Yong Susumu Ohtsuka Antiparasitic potential of Nephrolepis biserrata methanol extract against the parasitic leech Zeylanicobdella arugamensis (Hirudinea) and LC-QTOF analysis
description	Abstract Marine leech Zeylanicobdella arugamensis (Piscicolidae), an economically important parasite is infesting predominantly cultured groupers, hybrid groupers and other fish in Southeast Asian countries. In this study, we tested the anti-parasitic potential of a medicinal plant Nephrolepis biserrata found in Sabah, East Malaysia against Z. arugamensis. Various concentrations of methanol extracts of the plant were tested experimentally against Z. arugamensis and disinfestation of the leech from its primary host hybrid groupers. The composition of methanol extract of N. biserrata was determined through LC-QTOF analysis. The significant anti-parasitic activity of 100% mortality of leeches was observed with the exposure of N. biserrata extracts. The average time to kill the leeches at concentrations of 25, 50 and 100 mg/ml was 25.11 ± 3.26, 11.91 ± 0.99, and 4.88 ± 0.50 min., respectively. Further, at various low concentrations of N. biserrata 2.5, 5 and 10 mg/ml, hybrid groupers were disinfested in an average time of 108.33 ± 12.65, 65.83 ± 9.70 and 29.16 ± 5.85 min., respectively. The tandem mass spectrometry data from LC-QTOF indicated some hits on useful bioactive compounds such as terpenoids (ivalin, isovelleral, brassinolide, and eschscholtzxanthin), flavonoids (alnustin, kaempferol 7,4′-dimethyl ether, and pachypodol), phenolics (piscidic acid, chlorogenic acid, and ankorine), and aromatic (3-hydroxycoumarin). Thus N. biserrata can act as a potential biocontrol agent.
format	article
author	Muhammad Dawood Shah Balu Alagar Venmathi Maran Fatin Khairah Haron Julian Ransangan Fui Fui Ching Sitti Raehanah Muhamad Shaleh Rossita Shapawi Yoong Soon Yong Susumu Ohtsuka
author_facet	Muhammad Dawood Shah Balu Alagar Venmathi Maran Fatin Khairah Haron Julian Ransangan Fui Fui Ching Sitti Raehanah Muhamad Shaleh Rossita Shapawi Yoong Soon Yong Susumu Ohtsuka
author_sort	Muhammad Dawood Shah
title	Antiparasitic potential of Nephrolepis biserrata methanol extract against the parasitic leech Zeylanicobdella arugamensis (Hirudinea) and LC-QTOF analysis
title_short	Antiparasitic potential of Nephrolepis biserrata methanol extract against the parasitic leech Zeylanicobdella arugamensis (Hirudinea) and LC-QTOF analysis
title_full	Antiparasitic potential of Nephrolepis biserrata methanol extract against the parasitic leech Zeylanicobdella arugamensis (Hirudinea) and LC-QTOF analysis
title_fullStr	Antiparasitic potential of Nephrolepis biserrata methanol extract against the parasitic leech Zeylanicobdella arugamensis (Hirudinea) and LC-QTOF analysis
title_full_unstemmed	Antiparasitic potential of Nephrolepis biserrata methanol extract against the parasitic leech Zeylanicobdella arugamensis (Hirudinea) and LC-QTOF analysis
title_sort	antiparasitic potential of nephrolepis biserrata methanol extract against the parasitic leech zeylanicobdella arugamensis (hirudinea) and lc-qtof analysis
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/277a71bd0fc045ac8d6ea2181ceeae3a
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Antiparasitic potential of Nephrolepis biserrata methanol extract against the parasitic leech Zeylanicobdella arugamensis (Hirudinea) and LC-QTOF analysis

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