Poly(ethylmethacrylate-co-diethylaminoethyl acrylate) coating improves endothelial re-population, bio-mechanical and anti-thrombogenic properties of decellularized carotid arteries for blood vessel replacement

Abstract Decellularized vascular scaffolds are promising materials for vessel replacements. However, despite the natural origin of decellularized vessels, issues such as biomechanical incompatibility, immunogenicity risks and the hazards of thrombus formation, still need to be addressed. In this stu...

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Autores principales:	Elena López-Ruiz, Seshasailam Venkateswaran, Macarena Perán, Gema Jiménez, Salvatore Pernagallo, Juan J. Díaz-Mochón, Olga Tura-Ceide, Francisco Arrebola, Juan Melchor, Juan Soto, Guillermo Rus, Pedro J. Real, María Diaz-Ricart, Antonio Conde-González, Mark Bradley, Juan A. Marchal
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/277e4c5adca947e99621d87e4cd3c23a
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spelling	oai:doaj.org-article:277e4c5adca947e99621d87e4cd3c23a2021-12-02T11:52:39ZPoly(ethylmethacrylate-co-diethylaminoethyl acrylate) coating improves endothelial re-population, bio-mechanical and anti-thrombogenic properties of decellularized carotid arteries for blood vessel replacement10.1038/s41598-017-00294-62045-2322https://doaj.org/article/277e4c5adca947e99621d87e4cd3c23a2017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00294-6https://doaj.org/toc/2045-2322Abstract Decellularized vascular scaffolds are promising materials for vessel replacements. However, despite the natural origin of decellularized vessels, issues such as biomechanical incompatibility, immunogenicity risks and the hazards of thrombus formation, still need to be addressed. In this study, we coated decellularized vessels obtained from porcine carotid arteries with poly (ethylmethacrylate-co-diethylaminoethylacrylate) (8g7) with the purpose of improving endothelial coverage and minimizing platelet attachment while enhancing the mechanical properties of the decellularized vascular scaffolds. The polymer facilitated binding of endothelial cells (ECs) with high affinity and also induced endothelial cell capillary tube formation. In addition, platelets showed reduced adhesion on the polymer under flow conditions. Moreover, the coating of the decellularized arteries improved biomechanical properties by increasing its tensile strength and load. In addition, after 5 days in culture, ECs seeded on the luminal surface of 8g7-coated decellularized arteries showed good regeneration of the endothelium. Overall, this study shows that polymer coating of decellularized vessels provides a new strategy to improve re-endothelialization of vascular grafts, maintaining or enhancing mechanical properties while reducing the risk of thrombogenesis. These results could have potential applications in improving tissue-engineered vascular grafts for cardiovascular therapies with small caliber vessels.Elena López-RuizSeshasailam VenkateswaranMacarena PeránGema JiménezSalvatore PernagalloJuan J. Díaz-MochónOlga Tura-CeideFrancisco ArrebolaJuan MelchorJuan SotoGuillermo RusPedro J. RealMaría Diaz-RicartAntonio Conde-GonzálezMark BradleyJuan A. MarchalNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Elena López-Ruiz Seshasailam Venkateswaran Macarena Perán Gema Jiménez Salvatore Pernagallo Juan J. Díaz-Mochón Olga Tura-Ceide Francisco Arrebola Juan Melchor Juan Soto Guillermo Rus Pedro J. Real María Diaz-Ricart Antonio Conde-González Mark Bradley Juan A. Marchal Poly(ethylmethacrylate-co-diethylaminoethyl acrylate) coating improves endothelial re-population, bio-mechanical and anti-thrombogenic properties of decellularized carotid arteries for blood vessel replacement
description	Abstract Decellularized vascular scaffolds are promising materials for vessel replacements. However, despite the natural origin of decellularized vessels, issues such as biomechanical incompatibility, immunogenicity risks and the hazards of thrombus formation, still need to be addressed. In this study, we coated decellularized vessels obtained from porcine carotid arteries with poly (ethylmethacrylate-co-diethylaminoethylacrylate) (8g7) with the purpose of improving endothelial coverage and minimizing platelet attachment while enhancing the mechanical properties of the decellularized vascular scaffolds. The polymer facilitated binding of endothelial cells (ECs) with high affinity and also induced endothelial cell capillary tube formation. In addition, platelets showed reduced adhesion on the polymer under flow conditions. Moreover, the coating of the decellularized arteries improved biomechanical properties by increasing its tensile strength and load. In addition, after 5 days in culture, ECs seeded on the luminal surface of 8g7-coated decellularized arteries showed good regeneration of the endothelium. Overall, this study shows that polymer coating of decellularized vessels provides a new strategy to improve re-endothelialization of vascular grafts, maintaining or enhancing mechanical properties while reducing the risk of thrombogenesis. These results could have potential applications in improving tissue-engineered vascular grafts for cardiovascular therapies with small caliber vessels.
format	article
author	Elena López-Ruiz Seshasailam Venkateswaran Macarena Perán Gema Jiménez Salvatore Pernagallo Juan J. Díaz-Mochón Olga Tura-Ceide Francisco Arrebola Juan Melchor Juan Soto Guillermo Rus Pedro J. Real María Diaz-Ricart Antonio Conde-González Mark Bradley Juan A. Marchal
author_facet	Elena López-Ruiz Seshasailam Venkateswaran Macarena Perán Gema Jiménez Salvatore Pernagallo Juan J. Díaz-Mochón Olga Tura-Ceide Francisco Arrebola Juan Melchor Juan Soto Guillermo Rus Pedro J. Real María Diaz-Ricart Antonio Conde-González Mark Bradley Juan A. Marchal
author_sort	Elena López-Ruiz
title	Poly(ethylmethacrylate-co-diethylaminoethyl acrylate) coating improves endothelial re-population, bio-mechanical and anti-thrombogenic properties of decellularized carotid arteries for blood vessel replacement
title_short	Poly(ethylmethacrylate-co-diethylaminoethyl acrylate) coating improves endothelial re-population, bio-mechanical and anti-thrombogenic properties of decellularized carotid arteries for blood vessel replacement
title_full	Poly(ethylmethacrylate-co-diethylaminoethyl acrylate) coating improves endothelial re-population, bio-mechanical and anti-thrombogenic properties of decellularized carotid arteries for blood vessel replacement
title_fullStr	Poly(ethylmethacrylate-co-diethylaminoethyl acrylate) coating improves endothelial re-population, bio-mechanical and anti-thrombogenic properties of decellularized carotid arteries for blood vessel replacement
title_full_unstemmed	Poly(ethylmethacrylate-co-diethylaminoethyl acrylate) coating improves endothelial re-population, bio-mechanical and anti-thrombogenic properties of decellularized carotid arteries for blood vessel replacement
title_sort	poly(ethylmethacrylate-co-diethylaminoethyl acrylate) coating improves endothelial re-population, bio-mechanical and anti-thrombogenic properties of decellularized carotid arteries for blood vessel replacement
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/277e4c5adca947e99621d87e4cd3c23a
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Poly(ethylmethacrylate-co-diethylaminoethyl acrylate) coating improves endothelial re-population, bio-mechanical and anti-thrombogenic properties of decellularized carotid arteries for blood vessel replacement

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