Virus-encoded miRNAs in Ebola virus disease

Abstract Ebola virus (EBOV) is a negative-strand RNA virus that replicates in the cytoplasm and causes an often-fatal hemorrhagic fever. EBOV, like other viruses, can reportedly encode its own microRNAs (miRNAs) to subvert host immune defenses. miRNAs are short noncoding RNAs that can regulate gene...

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Autores principales:	Janice Duy, Anna N. Honko, Louis A. Altamura, Sandra L. Bixler, Suzanne Wollen-Roberts, Nadia Wauquier, Aileen O’Hearn, Eric M. Mucker, Joshua C. Johnson, Joshua D. Shamblin, Justine Zelko, Miriam A. Botto, James Bangura, Moinya Coomber, M. Louise Pitt, Jean-Paul Gonzalez, Randal J. Schoepp, Arthur J. Goff, Timothy D. Minogue
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/277f04bdc0d1417dae0bd78593c2b695
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spelling	oai:doaj.org-article:277f04bdc0d1417dae0bd78593c2b6952021-12-02T15:08:52ZVirus-encoded miRNAs in Ebola virus disease10.1038/s41598-018-23916-z2045-2322https://doaj.org/article/277f04bdc0d1417dae0bd78593c2b6952018-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-23916-zhttps://doaj.org/toc/2045-2322Abstract Ebola virus (EBOV) is a negative-strand RNA virus that replicates in the cytoplasm and causes an often-fatal hemorrhagic fever. EBOV, like other viruses, can reportedly encode its own microRNAs (miRNAs) to subvert host immune defenses. miRNAs are short noncoding RNAs that can regulate gene expression by hybridizing to multiple mRNAs, and viral miRNAs can enhance viral replication and infectivity by regulating host or viral genes. To date, only one EBOV miRNA has been examined in human infection. Here, we assayed mouse, rhesus macaque, cynomolgus macaque, and human samples infected with three EBOV variants for twelve computationally predicted viral miRNAs using RT-qPCR. Ten miRNAs aligned to EBOV variants and were detectable in the four species during disease with several viral miRNAs showing presymptomatic amplification in animal models. miRNA abundances in both the mouse and nonhuman primate models mirrored the human cohort, with miR-1-5p, miR-1-3p, and miR-T3-3p consistently at the highest levels. These striking similarities in the most abundant miRNAs during infection with different EBOV variants and hosts indicate that these miRNAs are potential valuable diagnostic markers and key effectors of EBOV pathogenesis.Janice DuyAnna N. HonkoLouis A. AltamuraSandra L. BixlerSuzanne Wollen-RobertsNadia WauquierAileen O’HearnEric M. MuckerJoshua C. JohnsonJoshua D. ShamblinJustine ZelkoMiriam A. BottoJames BanguraMoinya CoomberM. Louise PittJean-Paul GonzalezRandal J. SchoeppArthur J. GoffTimothy D. MinogueNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-14 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Janice Duy Anna N. Honko Louis A. Altamura Sandra L. Bixler Suzanne Wollen-Roberts Nadia Wauquier Aileen O’Hearn Eric M. Mucker Joshua C. Johnson Joshua D. Shamblin Justine Zelko Miriam A. Botto James Bangura Moinya Coomber M. Louise Pitt Jean-Paul Gonzalez Randal J. Schoepp Arthur J. Goff Timothy D. Minogue Virus-encoded miRNAs in Ebola virus disease
description	Abstract Ebola virus (EBOV) is a negative-strand RNA virus that replicates in the cytoplasm and causes an often-fatal hemorrhagic fever. EBOV, like other viruses, can reportedly encode its own microRNAs (miRNAs) to subvert host immune defenses. miRNAs are short noncoding RNAs that can regulate gene expression by hybridizing to multiple mRNAs, and viral miRNAs can enhance viral replication and infectivity by regulating host or viral genes. To date, only one EBOV miRNA has been examined in human infection. Here, we assayed mouse, rhesus macaque, cynomolgus macaque, and human samples infected with three EBOV variants for twelve computationally predicted viral miRNAs using RT-qPCR. Ten miRNAs aligned to EBOV variants and were detectable in the four species during disease with several viral miRNAs showing presymptomatic amplification in animal models. miRNA abundances in both the mouse and nonhuman primate models mirrored the human cohort, with miR-1-5p, miR-1-3p, and miR-T3-3p consistently at the highest levels. These striking similarities in the most abundant miRNAs during infection with different EBOV variants and hosts indicate that these miRNAs are potential valuable diagnostic markers and key effectors of EBOV pathogenesis.
format	article
author	Janice Duy Anna N. Honko Louis A. Altamura Sandra L. Bixler Suzanne Wollen-Roberts Nadia Wauquier Aileen O’Hearn Eric M. Mucker Joshua C. Johnson Joshua D. Shamblin Justine Zelko Miriam A. Botto James Bangura Moinya Coomber M. Louise Pitt Jean-Paul Gonzalez Randal J. Schoepp Arthur J. Goff Timothy D. Minogue
author_facet	Janice Duy Anna N. Honko Louis A. Altamura Sandra L. Bixler Suzanne Wollen-Roberts Nadia Wauquier Aileen O’Hearn Eric M. Mucker Joshua C. Johnson Joshua D. Shamblin Justine Zelko Miriam A. Botto James Bangura Moinya Coomber M. Louise Pitt Jean-Paul Gonzalez Randal J. Schoepp Arthur J. Goff Timothy D. Minogue
author_sort	Janice Duy
title	Virus-encoded miRNAs in Ebola virus disease
title_short	Virus-encoded miRNAs in Ebola virus disease
title_full	Virus-encoded miRNAs in Ebola virus disease
title_fullStr	Virus-encoded miRNAs in Ebola virus disease
title_full_unstemmed	Virus-encoded miRNAs in Ebola virus disease
title_sort	virus-encoded mirnas in ebola virus disease
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/277f04bdc0d1417dae0bd78593c2b695
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Virus-encoded miRNAs in Ebola virus disease

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