Mechanism and effect of curcumin on apoptosis of EAE mice by regulating TLRs/NF-κB signaling pathway
Objective: To investigate theanti apoptosis effect of curcumin (cur) in experimental autoimmune encephalomyelitis (EAE) mice by regulating TLRs/NF-κB signaling pathway and its mechanism. Methods: 45 C57BL/6 mice were randomly divided into the control group,EAE group,curcumin group ,15 mice in eac...
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Editorial Board of Journal of Hainan Medical University
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/27821defc1544e9e90aee05c1eb2f0c7 |
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| Sumario: | Objective: To investigate theanti apoptosis effect of curcumin (cur) in experimental
autoimmune encephalomyelitis (EAE) mice by regulating TLRs/NF-κB signaling pathway
and its mechanism. Methods: 45 C57BL/6 mice were randomly divided into the control
group,EAE group,curcumin group ,15 mice in each group. Blank groups are not processed. The
EAE model was established by classical modeling method in the EAE group and the curcumin
group. From the day of modeling, the blank group and the EAE group were intraperitoneally
injected with 1ml/kg/d of normal saline, Curcumin group was given 100 mg/kg/d continuous
intraperitoneal injection of curcumin extract. With Benson EAE group and Curcumin group
mice were killed at the peak of the disease. The blank group and the rest of the mice were
killed after 4 weeks of feeding, and the spinal cord tissue was taken out to separate the lumbar
enlargement segment. The effects of curcumin on the pathological changes of spinal cord
tissue in EAE mice were observed by HE staining and TUNEL staining, and the expression of
apoptosis positive cells was calculated. The distribution and co aggregation of apoptosis related
proteins Bcl-2 and Bax with spinal cord tissue were observed by double immunofluorescence
staining The protein levels of TLR4, NF-κBp65 and MyD88 were detected by Western blot.
Results: compared with the blank group, TUNEL staining increased the number of apoptotic
cells and the apoptotic rate in EAE group (P<0.05); the expression of apoptosis related protein
Bcl-2 decreased and the expression of Bax increased in EAE group (P<0.05) ,The protein of
TLR4, NF-κBp65 and MyD88 in spinal cord tissue of mice were increased by blot detection
(P<0.05); compared with EAE group, the number of apoptotic cells in spinal cord tissue of
curcumin group was decreased by TUNEL staining, and the apoptosis rate was decreased
(P<0.05); the expression of apoptosis related protein Bcl-2 was increased, the expression of
Bax protein was decreased, and Western blot was used to detect the expression of apoptosis
related protein The protein of TLR4, NF-κBp65 and MyD88 in spinal cord tissue of mice
were decreased by blot (P<0.05). Conclusion: Curcumin has anti apoptotic effect on EAE
mice, and its mechanism may be related to the inhibition of TLRs / NF-κB signaling pathway
and the reduction of apoptotic protein production. |
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