Exploring anticancer activity of wild and polyploid mutant of Artemisia cina

Abstract. Kasmiyati S, Kristiani EBE, Herawati MM, Rondonuwu FS. 2021. Exploring anticancer activity of wild and polyploid mutant of Artemisia cina. Biodiversitas 22: 1227-1234. The research aims were to explore the anticancer compounds in wild type (W) and polyploid mutant (P) of Artemisia cina Be...

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Autores principales:	Sri Kasmiyati, Elizabeth Betty Elok Kristiani, Maria Marina Herawati, Ferdy S Rondonuwu
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2021
Materias:	artemisia, cancer cell lines, cytotoxicity, selectivity indexes, anticancer compounds anticancer compounds Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/278731c558af441780ebc4a8f20b7b35
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spelling	oai:doaj.org-article:278731c558af441780ebc4a8f20b7b352021-11-22T00:56:37ZExploring anticancer activity of wild and polyploid mutant of Artemisia cina1412-033X2085-472210.13057/biodiv/d220319https://doaj.org/article/278731c558af441780ebc4a8f20b7b352021-02-01T00:00:00Zhttps://smujo.id/biodiv/article/view/7363https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Kasmiyati S, Kristiani EBE, Herawati MM, Rondonuwu FS. 2021. Exploring anticancer activity of wild and polyploid mutant of Artemisia cina. Biodiversitas 22: 1227-1234. The research aims were to explore the anticancer compounds in wild type (W) and polyploid mutant (P) of Artemisia cina Berg ex Poljakov using NIR and compare their cytotoxicity and selectivity on WiDR colon cancer and HTB183 lung cancer cell lines and Vero normal cell line. The W was obtained from B2P2TOOT Tawangmangu, Indonesia while P was by inducing A. cina shoot culture with 100 mg/l colchicine for 48 hours. They were extracted with hexane using the soxhletation method for 6 hours. The anticancer compounds were detected using NIR. The cytotoxic activity was determined using MTT assay, with Doxorubicin (D) as a control. The calculation of IC50 value used SPSS16 with a Probit analysis. The P contained the determined four compounds while the W contained no rutin. The IC50 values of W, P, and D were 295.5, 84.1, and 0.5 µg/mL on WiDr, 322.4, 128.6, and 39.9 µg/mL on HTB 183, whereas on Vero were 104, 315.6, and 295.5 µg/mL respectively. The selectivity indexes of W, P, and D were 34, 4, and 91 on WiDr, while HTB 183 was 31, 3, and 7, respectively. The P contained artemisinin, quercetin, kaemferol, and rutin, while the W contained no rutin. The cytotoxicity of both plants was less than doxorubicin. Both plants were selective on WiDR and HTB 183.Sri KasmiyatiElizabeth Betty Elok KristianiMaria Marina HerawatiFerdy S RondonuwuMBI & UNS Soloarticleartemisia, cancer cell lines, cytotoxicity, selectivity indexes, anticancer compounds anticancer compoundsBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 3 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	artemisia, cancer cell lines, cytotoxicity, selectivity indexes, anticancer compounds anticancer compounds Biology (General) QH301-705.5
spellingShingle	artemisia, cancer cell lines, cytotoxicity, selectivity indexes, anticancer compounds anticancer compounds Biology (General) QH301-705.5 Sri Kasmiyati Elizabeth Betty Elok Kristiani Maria Marina Herawati Ferdy S Rondonuwu Exploring anticancer activity of wild and polyploid mutant of Artemisia cina
description	Abstract. Kasmiyati S, Kristiani EBE, Herawati MM, Rondonuwu FS. 2021. Exploring anticancer activity of wild and polyploid mutant of Artemisia cina. Biodiversitas 22: 1227-1234. The research aims were to explore the anticancer compounds in wild type (W) and polyploid mutant (P) of Artemisia cina Berg ex Poljakov using NIR and compare their cytotoxicity and selectivity on WiDR colon cancer and HTB183 lung cancer cell lines and Vero normal cell line. The W was obtained from B2P2TOOT Tawangmangu, Indonesia while P was by inducing A. cina shoot culture with 100 mg/l colchicine for 48 hours. They were extracted with hexane using the soxhletation method for 6 hours. The anticancer compounds were detected using NIR. The cytotoxic activity was determined using MTT assay, with Doxorubicin (D) as a control. The calculation of IC50 value used SPSS16 with a Probit analysis. The P contained the determined four compounds while the W contained no rutin. The IC50 values of W, P, and D were 295.5, 84.1, and 0.5 µg/mL on WiDr, 322.4, 128.6, and 39.9 µg/mL on HTB 183, whereas on Vero were 104, 315.6, and 295.5 µg/mL respectively. The selectivity indexes of W, P, and D were 34, 4, and 91 on WiDr, while HTB 183 was 31, 3, and 7, respectively. The P contained artemisinin, quercetin, kaemferol, and rutin, while the W contained no rutin. The cytotoxicity of both plants was less than doxorubicin. Both plants were selective on WiDR and HTB 183.
format	article
author	Sri Kasmiyati Elizabeth Betty Elok Kristiani Maria Marina Herawati Ferdy S Rondonuwu
author_facet	Sri Kasmiyati Elizabeth Betty Elok Kristiani Maria Marina Herawati Ferdy S Rondonuwu
author_sort	Sri Kasmiyati
title	Exploring anticancer activity of wild and polyploid mutant of Artemisia cina
title_short	Exploring anticancer activity of wild and polyploid mutant of Artemisia cina
title_full	Exploring anticancer activity of wild and polyploid mutant of Artemisia cina
title_fullStr	Exploring anticancer activity of wild and polyploid mutant of Artemisia cina
title_full_unstemmed	Exploring anticancer activity of wild and polyploid mutant of Artemisia cina
title_sort	exploring anticancer activity of wild and polyploid mutant of artemisia cina
publisher	MBI & UNS Solo
publishDate	2021
url	https://doaj.org/article/278731c558af441780ebc4a8f20b7b35
work_keys_str_mv	AT srikasmiyati exploringanticanceractivityofwildandpolyploidmutantofartemisiacina AT elizabethbettyelokkristiani exploringanticanceractivityofwildandpolyploidmutantofartemisiacina AT mariamarinaherawati exploringanticanceractivityofwildandpolyploidmutantofartemisiacina AT ferdysrondonuwu exploringanticanceractivityofwildandpolyploidmutantofartemisiacina
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Exploring anticancer activity of wild and polyploid mutant of Artemisia cina

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