Influence of Plant Fraction, Soil, and Plant Species on Microbiota: a Multikingdom Comparison

ABSTRACT Plant roots influence the soil microbiota via physical interaction, secretion, and plant immunity. However, it is unclear whether the root fraction or soil is more important in determining the structure of the prokaryotic or eukaryotic community and whether this varies between plant species...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Andrzej Tkacz, Eloïne Bestion, Zhiyan Bo, Marion Hortala, Philip S. Poole
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2020
Materias:	microbial colonization plant microbiota rhizosphere roots phyllosphere colonization dynamics Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/2788c9d7b72f4ba38b23d9bce7659d99
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:2788c9d7b72f4ba38b23d9bce7659d99
record_format	dspace
spelling	oai:doaj.org-article:2788c9d7b72f4ba38b23d9bce7659d992021-11-15T15:56:58ZInfluence of Plant Fraction, Soil, and Plant Species on Microbiota: a Multikingdom Comparison10.1128/mBio.02785-192150-7511https://doaj.org/article/2788c9d7b72f4ba38b23d9bce7659d992020-02-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.02785-19https://doaj.org/toc/2150-7511ABSTRACT Plant roots influence the soil microbiota via physical interaction, secretion, and plant immunity. However, it is unclear whether the root fraction or soil is more important in determining the structure of the prokaryotic or eukaryotic community and whether this varies between plant species. Furthermore, the leaf (phyllosphere) and root microbiotas have a large overlap; however, it is unclear whether this results from colonization of the phyllosphere by the root microbiota. Soil, rhizosphere, rhizoplane, and root endosphere prokaryote-, eukaryote-, and fungus-specific microbiotas of four plant species were analyzed with high-throughput sequencing. The strengths of factors controlling microbiota structure were determined using permutational multivariate analysis of variance (PERMANOVA) statistics. The origin of the phyllosphere microbiota was investigated using a soil swap experiment. Global microbial kingdom analysis conducted simultaneously on multiple plants shows that cereals, legumes, and Brassicaceae establish similar prokaryotic and similar eukaryotic communities inside and on the root surface. While the bacterial microbiota is recruited from the surrounding soil, its profile is influenced by the root itself more so than by soil or plant species. However, in contrast, the fungal microbiota is most strongly influenced by soil. This was observed in two different soils and for all plant species examined. Microbiota structure is established within 2 weeks of plant growth in soil and remains stable thereafter. A reciprocal soil swap experiment shows that the phyllosphere is colonized from the soil in which the plant is grown. IMPORTANCE Global microbial kingdom analysis conducted simultaneously on multiple plants shows that cereals, legumes, and Brassicaceae establish similar prokaryotic and similar eukaryotic communities inside and on the root surface. While the bacterial microbiota is recruited from the surrounding soil, its profile is influenced by the root fraction more so than by soil or plant species. However, in contrast, the fungal microbiota is most strongly influenced by soil. This was observed in two different soils and for all plant species examined, indicating conserved adaptation of microbial communities to plants. Microbiota structure is established within 2 weeks of plant growth in soil and remains stable thereafter. We observed a remarkable similarity in the structure of a plant’s phyllosphere and root microbiotas and show by reciprocal soil swap experiments that both fractions are colonized from the soil in which the plant is grown. Thus, the phyllosphere is continuously colonized by the soil microbiota.Andrzej TkaczEloïne BestionZhiyan BoMarion HortalaPhilip S. PooleAmerican Society for Microbiologyarticlemicrobial colonizationplant microbiotarhizosphererootsphyllospherecolonization dynamicsMicrobiologyQR1-502ENmBio, Vol 11, Iss 1 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	microbial colonization plant microbiota rhizosphere roots phyllosphere colonization dynamics Microbiology QR1-502
spellingShingle	microbial colonization plant microbiota rhizosphere roots phyllosphere colonization dynamics Microbiology QR1-502 Andrzej Tkacz Eloïne Bestion Zhiyan Bo Marion Hortala Philip S. Poole Influence of Plant Fraction, Soil, and Plant Species on Microbiota: a Multikingdom Comparison
description	ABSTRACT Plant roots influence the soil microbiota via physical interaction, secretion, and plant immunity. However, it is unclear whether the root fraction or soil is more important in determining the structure of the prokaryotic or eukaryotic community and whether this varies between plant species. Furthermore, the leaf (phyllosphere) and root microbiotas have a large overlap; however, it is unclear whether this results from colonization of the phyllosphere by the root microbiota. Soil, rhizosphere, rhizoplane, and root endosphere prokaryote-, eukaryote-, and fungus-specific microbiotas of four plant species were analyzed with high-throughput sequencing. The strengths of factors controlling microbiota structure were determined using permutational multivariate analysis of variance (PERMANOVA) statistics. The origin of the phyllosphere microbiota was investigated using a soil swap experiment. Global microbial kingdom analysis conducted simultaneously on multiple plants shows that cereals, legumes, and Brassicaceae establish similar prokaryotic and similar eukaryotic communities inside and on the root surface. While the bacterial microbiota is recruited from the surrounding soil, its profile is influenced by the root itself more so than by soil or plant species. However, in contrast, the fungal microbiota is most strongly influenced by soil. This was observed in two different soils and for all plant species examined. Microbiota structure is established within 2 weeks of plant growth in soil and remains stable thereafter. A reciprocal soil swap experiment shows that the phyllosphere is colonized from the soil in which the plant is grown. IMPORTANCE Global microbial kingdom analysis conducted simultaneously on multiple plants shows that cereals, legumes, and Brassicaceae establish similar prokaryotic and similar eukaryotic communities inside and on the root surface. While the bacterial microbiota is recruited from the surrounding soil, its profile is influenced by the root fraction more so than by soil or plant species. However, in contrast, the fungal microbiota is most strongly influenced by soil. This was observed in two different soils and for all plant species examined, indicating conserved adaptation of microbial communities to plants. Microbiota structure is established within 2 weeks of plant growth in soil and remains stable thereafter. We observed a remarkable similarity in the structure of a plant’s phyllosphere and root microbiotas and show by reciprocal soil swap experiments that both fractions are colonized from the soil in which the plant is grown. Thus, the phyllosphere is continuously colonized by the soil microbiota.
format	article
author	Andrzej Tkacz Eloïne Bestion Zhiyan Bo Marion Hortala Philip S. Poole
author_facet	Andrzej Tkacz Eloïne Bestion Zhiyan Bo Marion Hortala Philip S. Poole
author_sort	Andrzej Tkacz
title	Influence of Plant Fraction, Soil, and Plant Species on Microbiota: a Multikingdom Comparison
title_short	Influence of Plant Fraction, Soil, and Plant Species on Microbiota: a Multikingdom Comparison
title_full	Influence of Plant Fraction, Soil, and Plant Species on Microbiota: a Multikingdom Comparison
title_fullStr	Influence of Plant Fraction, Soil, and Plant Species on Microbiota: a Multikingdom Comparison
title_full_unstemmed	Influence of Plant Fraction, Soil, and Plant Species on Microbiota: a Multikingdom Comparison
title_sort	influence of plant fraction, soil, and plant species on microbiota: a multikingdom comparison
publisher	American Society for Microbiology
publishDate	2020
url	https://doaj.org/article/2788c9d7b72f4ba38b23d9bce7659d99
work_keys_str_mv	AT andrzejtkacz influenceofplantfractionsoilandplantspeciesonmicrobiotaamultikingdomcomparison AT eloinebestion influenceofplantfractionsoilandplantspeciesonmicrobiotaamultikingdomcomparison AT zhiyanbo influenceofplantfractionsoilandplantspeciesonmicrobiotaamultikingdomcomparison AT marionhortala influenceofplantfractionsoilandplantspeciesonmicrobiotaamultikingdomcomparison AT philipspoole influenceofplantfractionsoilandplantspeciesonmicrobiotaamultikingdomcomparison
_version_	1718427076002840576

Influence of Plant Fraction, Soil, and Plant Species on Microbiota: a Multikingdom Comparison

Ejemplares similares