Identifications of immune-responsive genes for adaptative traits by comparative transcriptome analysis of spleen tissue from Kazakh and Suffolk sheep

Abstract Aridity and heat are significant environmental stressors that affect sheep adaptation and adaptability, thus influencing immunity, growth, reproduction, production performance, and profitability. The aim of this study was to profile mRNA expression levels in the spleen of indigenous Kazakh...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Hua Yang, Yong-Lin Yang, Guo-Qing Li, Qian Yu, Jinzeng Yang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/278d5c96a22641f98947e5b13aff4411
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:278d5c96a22641f98947e5b13aff4411
record_format	dspace
spelling	oai:doaj.org-article:278d5c96a22641f98947e5b13aff44112021-12-02T14:06:49ZIdentifications of immune-responsive genes for adaptative traits by comparative transcriptome analysis of spleen tissue from Kazakh and Suffolk sheep10.1038/s41598-021-82878-x2045-2322https://doaj.org/article/278d5c96a22641f98947e5b13aff44112021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82878-xhttps://doaj.org/toc/2045-2322Abstract Aridity and heat are significant environmental stressors that affect sheep adaptation and adaptability, thus influencing immunity, growth, reproduction, production performance, and profitability. The aim of this study was to profile mRNA expression levels in the spleen of indigenous Kazakh sheep breed for comparative analysis with the exotic Suffolk breed. Spleen histomorphology was observed in indigenous Kazakh sheep and exotic Suffolk sheep raised in Xinjiang China. Transcriptome sequencing of spleen tissue from the two breeds were performed via Illumina high-throughput sequencing technology and validated by RT-qPCR. Blood cytokine and IgG levels differed between the two breeds and IgG and IL-1β were significantly higher in Kazakh sheep than in Suffolk sheep (p < 0.05), though spleen tissue morphology was the same. A total of 52.04 Gb clean reads were obtained and the clean reads were assembled into 67,271 unigenes using bioinformatics analysis. Profiling analysis of differential gene expression showed that 1158 differentially expressed genes were found when comparing Suffolk with Kazakh sheep, including 246 up-regulated genes and 912 down-regulated genes. Utilizing gene ontology annotation and pathway analysis, 21 immune- responsive genes were identified as spleen-specific genes associated with adaptive traits and were significantly enriched in hematopoietic cell lineage, natural killer cell-mediated cytotoxicity, complement and coagulation cascades, and in the intestinal immune network for IgA production. Four pathways and up-regulated genes associated with immune responses in indigenous sheep played indispensable and promoting roles in arid and hot environments. Overall, this study provides valuable transcriptome data on the immunological mechanisms related to adaptive traits in indigenous and exotic sheep and offers a foundation for research into adaptive evolution.Hua YangYong-Lin YangGuo-Qing LiQian YuJinzeng YangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Hua Yang Yong-Lin Yang Guo-Qing Li Qian Yu Jinzeng Yang Identifications of immune-responsive genes for adaptative traits by comparative transcriptome analysis of spleen tissue from Kazakh and Suffolk sheep
description	Abstract Aridity and heat are significant environmental stressors that affect sheep adaptation and adaptability, thus influencing immunity, growth, reproduction, production performance, and profitability. The aim of this study was to profile mRNA expression levels in the spleen of indigenous Kazakh sheep breed for comparative analysis with the exotic Suffolk breed. Spleen histomorphology was observed in indigenous Kazakh sheep and exotic Suffolk sheep raised in Xinjiang China. Transcriptome sequencing of spleen tissue from the two breeds were performed via Illumina high-throughput sequencing technology and validated by RT-qPCR. Blood cytokine and IgG levels differed between the two breeds and IgG and IL-1β were significantly higher in Kazakh sheep than in Suffolk sheep (p < 0.05), though spleen tissue morphology was the same. A total of 52.04 Gb clean reads were obtained and the clean reads were assembled into 67,271 unigenes using bioinformatics analysis. Profiling analysis of differential gene expression showed that 1158 differentially expressed genes were found when comparing Suffolk with Kazakh sheep, including 246 up-regulated genes and 912 down-regulated genes. Utilizing gene ontology annotation and pathway analysis, 21 immune- responsive genes were identified as spleen-specific genes associated with adaptive traits and were significantly enriched in hematopoietic cell lineage, natural killer cell-mediated cytotoxicity, complement and coagulation cascades, and in the intestinal immune network for IgA production. Four pathways and up-regulated genes associated with immune responses in indigenous sheep played indispensable and promoting roles in arid and hot environments. Overall, this study provides valuable transcriptome data on the immunological mechanisms related to adaptive traits in indigenous and exotic sheep and offers a foundation for research into adaptive evolution.
format	article
author	Hua Yang Yong-Lin Yang Guo-Qing Li Qian Yu Jinzeng Yang
author_facet	Hua Yang Yong-Lin Yang Guo-Qing Li Qian Yu Jinzeng Yang
author_sort	Hua Yang
title	Identifications of immune-responsive genes for adaptative traits by comparative transcriptome analysis of spleen tissue from Kazakh and Suffolk sheep
title_short	Identifications of immune-responsive genes for adaptative traits by comparative transcriptome analysis of spleen tissue from Kazakh and Suffolk sheep
title_full	Identifications of immune-responsive genes for adaptative traits by comparative transcriptome analysis of spleen tissue from Kazakh and Suffolk sheep
title_fullStr	Identifications of immune-responsive genes for adaptative traits by comparative transcriptome analysis of spleen tissue from Kazakh and Suffolk sheep
title_full_unstemmed	Identifications of immune-responsive genes for adaptative traits by comparative transcriptome analysis of spleen tissue from Kazakh and Suffolk sheep
title_sort	identifications of immune-responsive genes for adaptative traits by comparative transcriptome analysis of spleen tissue from kazakh and suffolk sheep
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/278d5c96a22641f98947e5b13aff4411
work_keys_str_mv	AT huayang identificationsofimmuneresponsivegenesforadaptativetraitsbycomparativetranscriptomeanalysisofspleentissuefromkazakhandsuffolksheep AT yonglinyang identificationsofimmuneresponsivegenesforadaptativetraitsbycomparativetranscriptomeanalysisofspleentissuefromkazakhandsuffolksheep AT guoqingli identificationsofimmuneresponsivegenesforadaptativetraitsbycomparativetranscriptomeanalysisofspleentissuefromkazakhandsuffolksheep AT qianyu identificationsofimmuneresponsivegenesforadaptativetraitsbycomparativetranscriptomeanalysisofspleentissuefromkazakhandsuffolksheep AT jinzengyang identificationsofimmuneresponsivegenesforadaptativetraitsbycomparativetranscriptomeanalysisofspleentissuefromkazakhandsuffolksheep
_version_	1718391951394340864

Identifications of immune-responsive genes for adaptative traits by comparative transcriptome analysis of spleen tissue from Kazakh and Suffolk sheep

Ejemplares similares