Anti-Tumor Activity of Expanded PBMC-Derived NK Cells by Feeder-Free Protocol in Ovarian Cancer

Natural killer (NK) cells have shown great therapeutic potential against a wide range of cancers due to their pan-specific target recognition. Numerous reports indicate that NK cell immunotherapy is an effective therapeutic approach for treating hematological malignancies, but shows limited effects...

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Autores principales:	Minhua Chen, Yutong Li, Yu Wu, Siqi Xie, Jie Ma, Jingjing Yue, Rong Lv, Zhigang Tian, Fang Fang, Weihua Xiao
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	natural killer cell ovarian cancer ascites cell immunotherapy allogenic NK immunotherapy Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/2791ed452327405c9a47550744ca0ee7
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spelling	oai:doaj.org-article:2791ed452327405c9a47550744ca0ee72021-11-25T17:04:54ZAnti-Tumor Activity of Expanded PBMC-Derived NK Cells by Feeder-Free Protocol in Ovarian Cancer10.3390/cancers132258662072-6694https://doaj.org/article/2791ed452327405c9a47550744ca0ee72021-11-01T00:00:00Zhttps://www.mdpi.com/2072-6694/13/22/5866https://doaj.org/toc/2072-6694Natural killer (NK) cells have shown great therapeutic potential against a wide range of cancers due to their pan-specific target recognition. Numerous reports indicate that NK cell immunotherapy is an effective therapeutic approach for treating hematological malignancies, but shows limited effects against solid tumors. In this study, several models of ovarian cancer (OC) were used to test the anti-cancer effects of NK cells derived from human peripheral blood mononuclear cells and expanded using a feeder cell-free expansion system (eNKs). The results show that eNKs exhibit potent inhibitory activity on tumor growth in different ovarian cancer xenograft mice (i.e., solid tumors, abdominal metastatic tumors, and ascites), importantly, in a dose-dependent manner. Moreover, adoptive transfer of eNKs resulted in significant reduction in ascites formation in OC peritoneal tumor models, and especially in reducing intraperitoneal ascites. We found that eNKs could migrate to the tumor site, retain their activity, and proliferate to maintain high cell counts in cutaneous xenograft mice. In addition, when increased the infusion with a high dose of 12 × 10<sup>7</sup> cells/mouse, Graft-versus-host disease could be induced by eNK. These data show that eNK cell immunotherapy could be a promising treatment strategy for ovarian cancers, including solid tumors and ascites.Minhua ChenYutong LiYu WuSiqi XieJie MaJingjing YueRong LvZhigang TianFang FangWeihua XiaoMDPI AGarticlenatural killer cellovarian cancerascitescell immunotherapyallogenic NKimmunotherapyNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENCancers, Vol 13, Iss 5866, p 5866 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	natural killer cell ovarian cancer ascites cell immunotherapy allogenic NK immunotherapy Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	natural killer cell ovarian cancer ascites cell immunotherapy allogenic NK immunotherapy Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Minhua Chen Yutong Li Yu Wu Siqi Xie Jie Ma Jingjing Yue Rong Lv Zhigang Tian Fang Fang Weihua Xiao Anti-Tumor Activity of Expanded PBMC-Derived NK Cells by Feeder-Free Protocol in Ovarian Cancer
description	Natural killer (NK) cells have shown great therapeutic potential against a wide range of cancers due to their pan-specific target recognition. Numerous reports indicate that NK cell immunotherapy is an effective therapeutic approach for treating hematological malignancies, but shows limited effects against solid tumors. In this study, several models of ovarian cancer (OC) were used to test the anti-cancer effects of NK cells derived from human peripheral blood mononuclear cells and expanded using a feeder cell-free expansion system (eNKs). The results show that eNKs exhibit potent inhibitory activity on tumor growth in different ovarian cancer xenograft mice (i.e., solid tumors, abdominal metastatic tumors, and ascites), importantly, in a dose-dependent manner. Moreover, adoptive transfer of eNKs resulted in significant reduction in ascites formation in OC peritoneal tumor models, and especially in reducing intraperitoneal ascites. We found that eNKs could migrate to the tumor site, retain their activity, and proliferate to maintain high cell counts in cutaneous xenograft mice. In addition, when increased the infusion with a high dose of 12 × 10<sup>7</sup> cells/mouse, Graft-versus-host disease could be induced by eNK. These data show that eNK cell immunotherapy could be a promising treatment strategy for ovarian cancers, including solid tumors and ascites.
format	article
author	Minhua Chen Yutong Li Yu Wu Siqi Xie Jie Ma Jingjing Yue Rong Lv Zhigang Tian Fang Fang Weihua Xiao
author_facet	Minhua Chen Yutong Li Yu Wu Siqi Xie Jie Ma Jingjing Yue Rong Lv Zhigang Tian Fang Fang Weihua Xiao
author_sort	Minhua Chen
title	Anti-Tumor Activity of Expanded PBMC-Derived NK Cells by Feeder-Free Protocol in Ovarian Cancer
title_short	Anti-Tumor Activity of Expanded PBMC-Derived NK Cells by Feeder-Free Protocol in Ovarian Cancer
title_full	Anti-Tumor Activity of Expanded PBMC-Derived NK Cells by Feeder-Free Protocol in Ovarian Cancer
title_fullStr	Anti-Tumor Activity of Expanded PBMC-Derived NK Cells by Feeder-Free Protocol in Ovarian Cancer
title_full_unstemmed	Anti-Tumor Activity of Expanded PBMC-Derived NK Cells by Feeder-Free Protocol in Ovarian Cancer
title_sort	anti-tumor activity of expanded pbmc-derived nk cells by feeder-free protocol in ovarian cancer
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/2791ed452327405c9a47550744ca0ee7
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Anti-Tumor Activity of Expanded PBMC-Derived NK Cells by Feeder-Free Protocol in Ovarian Cancer

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