Route of infectious bronchitis virus vaccination determines the type and magnitude of immune responses in table egg laying hens

Abstract Chicken immune responses to infectious bronchitis virus (IBV) vaccination can depend on route of administration, vaccine strain and bird age. Typically for layer chickens, IBV vaccinations are administered by spray in the hatchery at day-old and boosted at intervals with live vaccines via d...

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Autores principales:	Mohammed Al-Rasheed, Christopher Ball, Kannan Ganapathy
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Avian coronavirus infectious bronchitis virus vaccination layer chicken gene transcription innate Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/279745f45c7241edba6f41fd93430c1d
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spelling	oai:doaj.org-article:279745f45c7241edba6f41fd93430c1d2021-11-14T12:07:48ZRoute of infectious bronchitis virus vaccination determines the type and magnitude of immune responses in table egg laying hens10.1186/s13567-021-01008-71297-9716https://doaj.org/article/279745f45c7241edba6f41fd93430c1d2021-11-01T00:00:00Zhttps://doi.org/10.1186/s13567-021-01008-7https://doaj.org/toc/1297-9716Abstract Chicken immune responses to infectious bronchitis virus (IBV) vaccination can depend on route of administration, vaccine strain and bird age. Typically for layer chickens, IBV vaccinations are administered by spray in the hatchery at day-old and boosted at intervals with live vaccines via drinking water (DW). Knowledge of live attenuated IBV vaccine virus kinetics and the immune response in egg-laying hens is exceptionally limited. Here, we demonstrated dissemination of vaccine viruses and differences in hen innate, mucosal, cellular and humoral immune responses following vaccination with Massachusetts or 793B strains, administered by DW or oculonasal (ON) routes. Detection of IBV in the Mass-vaccinated groups was greater during early time-points, however, 793B was detected more frequently at later timepoints. Viral RNA loads in the Harderian gland and turbinate tissues were significantly higher for ON-Mass compared to all other vaccinated groups. Lachrymal fluid IgY levels were significantly greater than the control at 14 days post-vaccination (dpv) for both vaccine serotypes, and IgA mRNA levels were significantly greater in ON-vaccinated groups compared to DW-vaccinated groups, demonstrating robust mucosal immune responses. Cell mediated immune gene transcripts (CD8-α and CD8-β) were up-regulated in turbinate and trachea tissues. For both vaccines, dissemination and vaccine virus clearance was slower when given by DW compared to the ON route. For ON administration, both vaccines induced comparable levels of mucosal immunity. The Mass vaccine induced cellular immunity to similar levels regardless of vaccination method. When given either by ON or DW, 793B vaccination induced significantly higher levels of humoral immunity.Mohammed Al-RasheedChristopher BallKannan GanapathyBMCarticleAvian coronavirusinfectious bronchitis virusvaccinationlayer chickengene transcriptioninnateVeterinary medicineSF600-1100ENVeterinary Research, Vol 52, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Avian coronavirus infectious bronchitis virus vaccination layer chicken gene transcription innate Veterinary medicine SF600-1100
spellingShingle	Avian coronavirus infectious bronchitis virus vaccination layer chicken gene transcription innate Veterinary medicine SF600-1100 Mohammed Al-Rasheed Christopher Ball Kannan Ganapathy Route of infectious bronchitis virus vaccination determines the type and magnitude of immune responses in table egg laying hens
description	Abstract Chicken immune responses to infectious bronchitis virus (IBV) vaccination can depend on route of administration, vaccine strain and bird age. Typically for layer chickens, IBV vaccinations are administered by spray in the hatchery at day-old and boosted at intervals with live vaccines via drinking water (DW). Knowledge of live attenuated IBV vaccine virus kinetics and the immune response in egg-laying hens is exceptionally limited. Here, we demonstrated dissemination of vaccine viruses and differences in hen innate, mucosal, cellular and humoral immune responses following vaccination with Massachusetts or 793B strains, administered by DW or oculonasal (ON) routes. Detection of IBV in the Mass-vaccinated groups was greater during early time-points, however, 793B was detected more frequently at later timepoints. Viral RNA loads in the Harderian gland and turbinate tissues were significantly higher for ON-Mass compared to all other vaccinated groups. Lachrymal fluid IgY levels were significantly greater than the control at 14 days post-vaccination (dpv) for both vaccine serotypes, and IgA mRNA levels were significantly greater in ON-vaccinated groups compared to DW-vaccinated groups, demonstrating robust mucosal immune responses. Cell mediated immune gene transcripts (CD8-α and CD8-β) were up-regulated in turbinate and trachea tissues. For both vaccines, dissemination and vaccine virus clearance was slower when given by DW compared to the ON route. For ON administration, both vaccines induced comparable levels of mucosal immunity. The Mass vaccine induced cellular immunity to similar levels regardless of vaccination method. When given either by ON or DW, 793B vaccination induced significantly higher levels of humoral immunity.
format	article
author	Mohammed Al-Rasheed Christopher Ball Kannan Ganapathy
author_facet	Mohammed Al-Rasheed Christopher Ball Kannan Ganapathy
author_sort	Mohammed Al-Rasheed
title	Route of infectious bronchitis virus vaccination determines the type and magnitude of immune responses in table egg laying hens
title_short	Route of infectious bronchitis virus vaccination determines the type and magnitude of immune responses in table egg laying hens
title_full	Route of infectious bronchitis virus vaccination determines the type and magnitude of immune responses in table egg laying hens
title_fullStr	Route of infectious bronchitis virus vaccination determines the type and magnitude of immune responses in table egg laying hens
title_full_unstemmed	Route of infectious bronchitis virus vaccination determines the type and magnitude of immune responses in table egg laying hens
title_sort	route of infectious bronchitis virus vaccination determines the type and magnitude of immune responses in table egg laying hens
publisher	BMC
publishDate	2021
url	https://doaj.org/article/279745f45c7241edba6f41fd93430c1d
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Route of infectious bronchitis virus vaccination determines the type and magnitude of immune responses in table egg laying hens

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