Inverse opal substrate-loaded mesenchymal stem cells contribute to decreased myocardial remodeling after transplantation into acute myocardial infarction mice

Wenbin Lu,1,* JingJing Ji,1,* Genshan Ma,1 Qiming Dai,1 Lijuan Chen,1 Pengfei Zuo,1 Yuanjin Zhao2 1Department of Cardiology, ZhongDa Hospital Affiliated with Southeast University, Nanjing, China; 2State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeas...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lu W, Ji J, Ma G, Dai Q, Chen L, Zuo P, Zhao Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	MSCs Inverse opal AMI. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/279fb642c0cb439ba8ec6f87bcc88e27
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:279fb642c0cb439ba8ec6f87bcc88e27
record_format	dspace
spelling	oai:doaj.org-article:279fb642c0cb439ba8ec6f87bcc88e272021-12-02T04:56:13ZInverse opal substrate-loaded mesenchymal stem cells contribute to decreased myocardial remodeling after transplantation into acute myocardial infarction mice1178-2013https://doaj.org/article/279fb642c0cb439ba8ec6f87bcc88e272018-11-01T00:00:00Zhttps://www.dovepress.com/inverse-opal-substrate-loaded-mesenchymal-stem-cells-contribute-to-dec-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Wenbin Lu,1,* JingJing Ji,1,* Genshan Ma,1 Qiming Dai,1 Lijuan Chen,1 Pengfei Zuo,1 Yuanjin Zhao2 1Department of Cardiology, ZhongDa Hospital Affiliated with Southeast University, Nanjing, China; 2State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China *These authors contributed equally to this work Background: The two-dimensional incubation method is now the most commonly method for mesenchymal stem cell (MSC) production. however, gene expression and secretion of growth factors are relatively low; thus, the transplanted cells cannot be effectively utilized for potential clinical applications after acute myocardial infarction (AMI).Objectives: We aimed to investigate whether our newly made substrates of inverse opal with specific surface microstructures for MSC culturing can increase the viability of the cells and can contributes to decreased myocardial remodeling after transplanted to AMI mice. Methods: The inverse opal structure is fabricated by the convenient bottom-up approach of the self-assembly of colloidal nanoparticles. Mouse-derived MSCs were then cultured on the substrates when expanded at different times to investigate the cell growth status including morphology. Then the inverse opal substrates loaded MSCs were transplanted to AMI mice, cardiomyocyte apoptosis and LV remodeling were further compared. To explore the possible mechanisms of curation, the secretions and viability of MSCs on substrates were determined using mice ELISA kits and JC-1 mitochondrial membrane potential assay kits respectively at normal and hypoxic conditions. Results: 6 times expanded inverse opals allowed greatly the orderly growth of MSCs as compared to four (34% ± 10.6%) and two (20%±7.2%) times expanded as well as unexpanded (13%±4.1%) (P<0.001). Nearly 90% of MSCs showed orientation angle intervals of less than 30° when at the 6X expanded (89.6%±25%) compared to the percent of cells with 30°–60° (8.7%±2.6%) or ≥60° (1.7%±1.0%) orientation angle (P<0.001). After inverse opal loaded MSCs transplanted to AMI mice, greatly decreased apoptosis of cardiomyocytes (20.45%±8.64% vs.39.63%±11.71%, P<0.001) and infarction area (5.87±2.18 mm2 vs 9.31±3.11mm2, P<0.001) were identified. In the end, the viability of inverse opal loaded MSCs determined by membrane potential (P<0.001) and the secretion of growth factors including VEGF-α, SDF-1 and Ang-1 (P<0.001) were both confirmed significantly higher than that of the conventional culture in petri dish.Conclusion: The structure of inverse opal can not only adjust the arrangement of MSCs but also contribute to its orientated growth. Inverse opal loaded MSCs transplantation extremely curbed myocardial remodeling, the underlying mechanisms might be the high viability and extremely higher secretions of growth factors of MSCs as devoted by this method. Keywords: MSCs, inverse opal, AMI Lu WJi JMa GDai QChen LZuo PZhao YDove Medical PressarticleMSCsInverse opalAMI.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 7033-7046 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	MSCs Inverse opal AMI. Medicine (General) R5-920
spellingShingle	MSCs Inverse opal AMI. Medicine (General) R5-920 Lu W Ji J Ma G Dai Q Chen L Zuo P Zhao Y Inverse opal substrate-loaded mesenchymal stem cells contribute to decreased myocardial remodeling after transplantation into acute myocardial infarction mice
description	Wenbin Lu,1,* JingJing Ji,1,* Genshan Ma,1 Qiming Dai,1 Lijuan Chen,1 Pengfei Zuo,1 Yuanjin Zhao2 1Department of Cardiology, ZhongDa Hospital Affiliated with Southeast University, Nanjing, China; 2State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China *These authors contributed equally to this work Background: The two-dimensional incubation method is now the most commonly method for mesenchymal stem cell (MSC) production. however, gene expression and secretion of growth factors are relatively low; thus, the transplanted cells cannot be effectively utilized for potential clinical applications after acute myocardial infarction (AMI).Objectives: We aimed to investigate whether our newly made substrates of inverse opal with specific surface microstructures for MSC culturing can increase the viability of the cells and can contributes to decreased myocardial remodeling after transplanted to AMI mice. Methods: The inverse opal structure is fabricated by the convenient bottom-up approach of the self-assembly of colloidal nanoparticles. Mouse-derived MSCs were then cultured on the substrates when expanded at different times to investigate the cell growth status including morphology. Then the inverse opal substrates loaded MSCs were transplanted to AMI mice, cardiomyocyte apoptosis and LV remodeling were further compared. To explore the possible mechanisms of curation, the secretions and viability of MSCs on substrates were determined using mice ELISA kits and JC-1 mitochondrial membrane potential assay kits respectively at normal and hypoxic conditions. Results: 6 times expanded inverse opals allowed greatly the orderly growth of MSCs as compared to four (34% ± 10.6%) and two (20%±7.2%) times expanded as well as unexpanded (13%±4.1%) (P<0.001). Nearly 90% of MSCs showed orientation angle intervals of less than 30° when at the 6X expanded (89.6%±25%) compared to the percent of cells with 30°–60° (8.7%±2.6%) or ≥60° (1.7%±1.0%) orientation angle (P<0.001). After inverse opal loaded MSCs transplanted to AMI mice, greatly decreased apoptosis of cardiomyocytes (20.45%±8.64% vs.39.63%±11.71%, P<0.001) and infarction area (5.87±2.18 mm2 vs 9.31±3.11mm2, P<0.001) were identified. In the end, the viability of inverse opal loaded MSCs determined by membrane potential (P<0.001) and the secretion of growth factors including VEGF-α, SDF-1 and Ang-1 (P<0.001) were both confirmed significantly higher than that of the conventional culture in petri dish.Conclusion: The structure of inverse opal can not only adjust the arrangement of MSCs but also contribute to its orientated growth. Inverse opal loaded MSCs transplantation extremely curbed myocardial remodeling, the underlying mechanisms might be the high viability and extremely higher secretions of growth factors of MSCs as devoted by this method. Keywords: MSCs, inverse opal, AMI
format	article
author	Lu W Ji J Ma G Dai Q Chen L Zuo P Zhao Y
author_facet	Lu W Ji J Ma G Dai Q Chen L Zuo P Zhao Y
author_sort	Lu W
title	Inverse opal substrate-loaded mesenchymal stem cells contribute to decreased myocardial remodeling after transplantation into acute myocardial infarction mice
title_short	Inverse opal substrate-loaded mesenchymal stem cells contribute to decreased myocardial remodeling after transplantation into acute myocardial infarction mice
title_full	Inverse opal substrate-loaded mesenchymal stem cells contribute to decreased myocardial remodeling after transplantation into acute myocardial infarction mice
title_fullStr	Inverse opal substrate-loaded mesenchymal stem cells contribute to decreased myocardial remodeling after transplantation into acute myocardial infarction mice
title_full_unstemmed	Inverse opal substrate-loaded mesenchymal stem cells contribute to decreased myocardial remodeling after transplantation into acute myocardial infarction mice
title_sort	inverse opal substrate-loaded mesenchymal stem cells contribute to decreased myocardial remodeling after transplantation into acute myocardial infarction mice
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/279fb642c0cb439ba8ec6f87bcc88e27
work_keys_str_mv	AT luw inverseopalsubstrateloadedmesenchymalstemcellscontributetodecreasedmyocardialremodelingaftertransplantationintoacutemyocardialinfarctionmice AT jij inverseopalsubstrateloadedmesenchymalstemcellscontributetodecreasedmyocardialremodelingaftertransplantationintoacutemyocardialinfarctionmice AT mag inverseopalsubstrateloadedmesenchymalstemcellscontributetodecreasedmyocardialremodelingaftertransplantationintoacutemyocardialinfarctionmice AT daiq inverseopalsubstrateloadedmesenchymalstemcellscontributetodecreasedmyocardialremodelingaftertransplantationintoacutemyocardialinfarctionmice AT chenl inverseopalsubstrateloadedmesenchymalstemcellscontributetodecreasedmyocardialremodelingaftertransplantationintoacutemyocardialinfarctionmice AT zuop inverseopalsubstrateloadedmesenchymalstemcellscontributetodecreasedmyocardialremodelingaftertransplantationintoacutemyocardialinfarctionmice AT zhaoy inverseopalsubstrateloadedmesenchymalstemcellscontributetodecreasedmyocardialremodelingaftertransplantationintoacutemyocardialinfarctionmice
_version_	1718400937561686016

Inverse opal substrate-loaded mesenchymal stem cells contribute to decreased myocardial remodeling after transplantation into acute myocardial infarction mice

Ejemplares similares