Hyaluronic Acid as a Carrier Supports the Effects of Glucocorticoids and Diminishes the Cytotoxic Effects of Local Anesthetics in Human Articular Chondrocytes In Vitro

The current study aimed to investigate the cytotoxicity of co-administrating local anesthetics (LA) with glucocorticoids (GC) and hyaluronic acid (HA) in vitro. Human articular cartilage was obtained from five patients undergoing total knee arthroplasty. Chondrocytes were isolated, expanded, and see...

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Autores principales:	Lukas B. Moser, Christoph Bauer, Vivek Jeyakumar, Eugenia-Paulina Niculescu-Morzsa, Stefan Nehrer
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	hyaluronic acid glucocorticoids osteoarthritis viscosupplementation intra-articular injections Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/27a4ee18ce36417c864447deace5e8cc
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spelling	oai:doaj.org-article:27a4ee18ce36417c864447deace5e8cc2021-11-11T16:57:55ZHyaluronic Acid as a Carrier Supports the Effects of Glucocorticoids and Diminishes the Cytotoxic Effects of Local Anesthetics in Human Articular Chondrocytes In Vitro10.3390/ijms2221115031422-00671661-6596https://doaj.org/article/27a4ee18ce36417c864447deace5e8cc2021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11503https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067The current study aimed to investigate the cytotoxicity of co-administrating local anesthetics (LA) with glucocorticoids (GC) and hyaluronic acid (HA) in vitro. Human articular cartilage was obtained from five patients undergoing total knee arthroplasty. Chondrocytes were isolated, expanded, and seeded in 24-well plates for experimental testing. LA (lidocaine, bupivacaine, ropivacaine) were administered separately and co-administered with the following substances: GC, HA, and GC/HA. Viability was confirmed by microscopic images, flow cytometry, metabolic activity, and live/dead assay. The addition of HA and GC/HA resulted in enhanced attachment and branched appearance of the chondrocytes compared to LA and LA/GC. Metabolic activity was better in all LA co-administered with HA and GC/HA than with GC and only LA. Flow cytometry revealed the lowest cell viability in lidocaine and the highest cell viability in ropivacaine. This finding was also confirmed by live/dead assay. In conclusion, HA supports the effect of GC and reduces chondrotoxic effects of LA in vitro. Thereby, the co-administration of HA to LA and GC offers an alternative less chondrotoxic approach for treating patients with symptomatic osteoarthritis of the knee.Lukas B. MoserChristoph BauerVivek JeyakumarEugenia-Paulina Niculescu-MorzsaStefan NehrerMDPI AGarticlehyaluronic acidglucocorticoidsosteoarthritisviscosupplementationintra-articularinjectionsBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11503, p 11503 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	hyaluronic acid glucocorticoids osteoarthritis viscosupplementation intra-articular injections Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	hyaluronic acid glucocorticoids osteoarthritis viscosupplementation intra-articular injections Biology (General) QH301-705.5 Chemistry QD1-999 Lukas B. Moser Christoph Bauer Vivek Jeyakumar Eugenia-Paulina Niculescu-Morzsa Stefan Nehrer Hyaluronic Acid as a Carrier Supports the Effects of Glucocorticoids and Diminishes the Cytotoxic Effects of Local Anesthetics in Human Articular Chondrocytes In Vitro
description	The current study aimed to investigate the cytotoxicity of co-administrating local anesthetics (LA) with glucocorticoids (GC) and hyaluronic acid (HA) in vitro. Human articular cartilage was obtained from five patients undergoing total knee arthroplasty. Chondrocytes were isolated, expanded, and seeded in 24-well plates for experimental testing. LA (lidocaine, bupivacaine, ropivacaine) were administered separately and co-administered with the following substances: GC, HA, and GC/HA. Viability was confirmed by microscopic images, flow cytometry, metabolic activity, and live/dead assay. The addition of HA and GC/HA resulted in enhanced attachment and branched appearance of the chondrocytes compared to LA and LA/GC. Metabolic activity was better in all LA co-administered with HA and GC/HA than with GC and only LA. Flow cytometry revealed the lowest cell viability in lidocaine and the highest cell viability in ropivacaine. This finding was also confirmed by live/dead assay. In conclusion, HA supports the effect of GC and reduces chondrotoxic effects of LA in vitro. Thereby, the co-administration of HA to LA and GC offers an alternative less chondrotoxic approach for treating patients with symptomatic osteoarthritis of the knee.
format	article
author	Lukas B. Moser Christoph Bauer Vivek Jeyakumar Eugenia-Paulina Niculescu-Morzsa Stefan Nehrer
author_facet	Lukas B. Moser Christoph Bauer Vivek Jeyakumar Eugenia-Paulina Niculescu-Morzsa Stefan Nehrer
author_sort	Lukas B. Moser
title	Hyaluronic Acid as a Carrier Supports the Effects of Glucocorticoids and Diminishes the Cytotoxic Effects of Local Anesthetics in Human Articular Chondrocytes In Vitro
title_short	Hyaluronic Acid as a Carrier Supports the Effects of Glucocorticoids and Diminishes the Cytotoxic Effects of Local Anesthetics in Human Articular Chondrocytes In Vitro
title_full	Hyaluronic Acid as a Carrier Supports the Effects of Glucocorticoids and Diminishes the Cytotoxic Effects of Local Anesthetics in Human Articular Chondrocytes In Vitro
title_fullStr	Hyaluronic Acid as a Carrier Supports the Effects of Glucocorticoids and Diminishes the Cytotoxic Effects of Local Anesthetics in Human Articular Chondrocytes In Vitro
title_full_unstemmed	Hyaluronic Acid as a Carrier Supports the Effects of Glucocorticoids and Diminishes the Cytotoxic Effects of Local Anesthetics in Human Articular Chondrocytes In Vitro
title_sort	hyaluronic acid as a carrier supports the effects of glucocorticoids and diminishes the cytotoxic effects of local anesthetics in human articular chondrocytes in vitro
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/27a4ee18ce36417c864447deace5e8cc
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Hyaluronic Acid as a Carrier Supports the Effects of Glucocorticoids and Diminishes the Cytotoxic Effects of Local Anesthetics in Human Articular Chondrocytes In Vitro

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