Re-examination of the APETALA2/Ethylene-Responsive Factor Gene Family in Barley (Hordeum vulgare L.) Indicates a Role in the Regulation of Starch Synthesis

The APETALA2/Ethylene-Responsive factor (AP2/ERF) gene family is a large plant-specific transcription factor family, which plays important roles in regulating plant growth and development. A role in starch synthesis is among the multiple functions of this family of transcription factors. Barley (Hor...

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Autores principales: Jinjin Ding, Hassan Karim, Yulong Li, Wendy Harwood, Carlos Guzmán, Na Lin, Qiang Xu, Yazhou Zhang, Huaping Tang, Yunfeng Jiang, Pengfei Qi, Mei Deng, Jian Ma, Jirui Wang, Guoyue Chen, Xiujin Lan, Yuming Wei, Youliang Zheng, Qiantao Jiang
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Publicado: Frontiers Media S.A. 2021
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spelling oai:doaj.org-article:27b5f7f0e4aa4a179794aef69168de2f2021-12-02T00:33:56ZRe-examination of the APETALA2/Ethylene-Responsive Factor Gene Family in Barley (Hordeum vulgare L.) Indicates a Role in the Regulation of Starch Synthesis1664-462X10.3389/fpls.2021.791584https://doaj.org/article/27b5f7f0e4aa4a179794aef69168de2f2021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.791584/fullhttps://doaj.org/toc/1664-462XThe APETALA2/Ethylene-Responsive factor (AP2/ERF) gene family is a large plant-specific transcription factor family, which plays important roles in regulating plant growth and development. A role in starch synthesis is among the multiple functions of this family of transcription factors. Barley (Hordeum vulgare L.) is one of the most important cereals for starch production. However, there are limited data on the contribution of AP2 transcription factors in barley. In this study, we used the recently published barley genome database (Morex) to identify 185 genes of the HvAP2/ERF family. Compared with previous work, we identified 64 new genes in the HvAP2/ERF gene family and corrected some previously misannotated and duplicated genes. After phylogenetic analysis, HvAP2/ERF genes were classified into four subfamilies and 18 subgroups. Expression profiling showed different patterns of spatial and temporal expression for HvAP2/ERF genes. Most of the 12 HvAP2/ERF genes analyzed using quantitative reverse transcription–polymerase chain reaction had similar expression patterns when compared with those of starch synthase genes in barley, except for HvAP2-18 and HvERF-73. HvAP2-18 is homologous to OsRSR1, which negatively regulates the synthesis of rice starch. Luciferase reporter gene, and yeast one-hybrid assays showed that HvAP2-18 bound the promoter of AGP-S and SBE1 in vitro. Thus, HvAP2-18 might be an interesting candidate gene to further explore the mechanisms involved in the regulation of starch synthesis in barley.Jinjin DingJinjin DingHassan KarimHassan KarimYulong LiYulong LiWendy HarwoodCarlos GuzmánNa LinQiang XuQiang XuYazhou ZhangYazhou ZhangHuaping TangHuaping TangYunfeng JiangYunfeng JiangPengfei QiPengfei QiMei DengMei DengJian MaJian MaJirui WangJirui WangGuoyue ChenGuoyue ChenXiujin LanXiujin LanYuming WeiYuming WeiYouliang ZhengYouliang ZhengQiantao JiangQiantao JiangFrontiers Media S.A.articlebarleyAPETALA2/Ethylene-Responsivetranscription factorsgene interactionstarch synthesisPlant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution DOAJ
collection DOAJ
language EN
topic barley
APETALA2/Ethylene-Responsive
transcription factors
gene interaction
starch synthesis
Plant culture
SB1-1110
spellingShingle barley
APETALA2/Ethylene-Responsive
transcription factors
gene interaction
starch synthesis
Plant culture
SB1-1110
Jinjin Ding
Jinjin Ding
Hassan Karim
Hassan Karim
Yulong Li
Yulong Li
Wendy Harwood
Carlos Guzmán
Na Lin
Qiang Xu
Qiang Xu
Yazhou Zhang
Yazhou Zhang
Huaping Tang
Huaping Tang
Yunfeng Jiang
Yunfeng Jiang
Pengfei Qi
Pengfei Qi
Mei Deng
Mei Deng
Jian Ma
Jian Ma
Jirui Wang
Jirui Wang
Guoyue Chen
Guoyue Chen
Xiujin Lan
Xiujin Lan
Yuming Wei
Yuming Wei
Youliang Zheng
Youliang Zheng
Qiantao Jiang
Qiantao Jiang
Re-examination of the APETALA2/Ethylene-Responsive Factor Gene Family in Barley (Hordeum vulgare L.) Indicates a Role in the Regulation of Starch Synthesis
description The APETALA2/Ethylene-Responsive factor (AP2/ERF) gene family is a large plant-specific transcription factor family, which plays important roles in regulating plant growth and development. A role in starch synthesis is among the multiple functions of this family of transcription factors. Barley (Hordeum vulgare L.) is one of the most important cereals for starch production. However, there are limited data on the contribution of AP2 transcription factors in barley. In this study, we used the recently published barley genome database (Morex) to identify 185 genes of the HvAP2/ERF family. Compared with previous work, we identified 64 new genes in the HvAP2/ERF gene family and corrected some previously misannotated and duplicated genes. After phylogenetic analysis, HvAP2/ERF genes were classified into four subfamilies and 18 subgroups. Expression profiling showed different patterns of spatial and temporal expression for HvAP2/ERF genes. Most of the 12 HvAP2/ERF genes analyzed using quantitative reverse transcription–polymerase chain reaction had similar expression patterns when compared with those of starch synthase genes in barley, except for HvAP2-18 and HvERF-73. HvAP2-18 is homologous to OsRSR1, which negatively regulates the synthesis of rice starch. Luciferase reporter gene, and yeast one-hybrid assays showed that HvAP2-18 bound the promoter of AGP-S and SBE1 in vitro. Thus, HvAP2-18 might be an interesting candidate gene to further explore the mechanisms involved in the regulation of starch synthesis in barley.
format article
author Jinjin Ding
Jinjin Ding
Hassan Karim
Hassan Karim
Yulong Li
Yulong Li
Wendy Harwood
Carlos Guzmán
Na Lin
Qiang Xu
Qiang Xu
Yazhou Zhang
Yazhou Zhang
Huaping Tang
Huaping Tang
Yunfeng Jiang
Yunfeng Jiang
Pengfei Qi
Pengfei Qi
Mei Deng
Mei Deng
Jian Ma
Jian Ma
Jirui Wang
Jirui Wang
Guoyue Chen
Guoyue Chen
Xiujin Lan
Xiujin Lan
Yuming Wei
Yuming Wei
Youliang Zheng
Youliang Zheng
Qiantao Jiang
Qiantao Jiang
author_facet Jinjin Ding
Jinjin Ding
Hassan Karim
Hassan Karim
Yulong Li
Yulong Li
Wendy Harwood
Carlos Guzmán
Na Lin
Qiang Xu
Qiang Xu
Yazhou Zhang
Yazhou Zhang
Huaping Tang
Huaping Tang
Yunfeng Jiang
Yunfeng Jiang
Pengfei Qi
Pengfei Qi
Mei Deng
Mei Deng
Jian Ma
Jian Ma
Jirui Wang
Jirui Wang
Guoyue Chen
Guoyue Chen
Xiujin Lan
Xiujin Lan
Yuming Wei
Yuming Wei
Youliang Zheng
Youliang Zheng
Qiantao Jiang
Qiantao Jiang
author_sort Jinjin Ding
title Re-examination of the APETALA2/Ethylene-Responsive Factor Gene Family in Barley (Hordeum vulgare L.) Indicates a Role in the Regulation of Starch Synthesis
title_short Re-examination of the APETALA2/Ethylene-Responsive Factor Gene Family in Barley (Hordeum vulgare L.) Indicates a Role in the Regulation of Starch Synthesis
title_full Re-examination of the APETALA2/Ethylene-Responsive Factor Gene Family in Barley (Hordeum vulgare L.) Indicates a Role in the Regulation of Starch Synthesis
title_fullStr Re-examination of the APETALA2/Ethylene-Responsive Factor Gene Family in Barley (Hordeum vulgare L.) Indicates a Role in the Regulation of Starch Synthesis
title_full_unstemmed Re-examination of the APETALA2/Ethylene-Responsive Factor Gene Family in Barley (Hordeum vulgare L.) Indicates a Role in the Regulation of Starch Synthesis
title_sort re-examination of the apetala2/ethylene-responsive factor gene family in barley (hordeum vulgare l.) indicates a role in the regulation of starch synthesis
publisher Frontiers Media S.A.
publishDate 2021
url https://doaj.org/article/27b5f7f0e4aa4a179794aef69168de2f
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