Cysteine: an overlooked energy and carbon source

Abstract Biohybrids composed of microorganisms and nanoparticles have emerged as potential systems for bioenergy and high-value compound production from CO2 and light energy, yet the cellular and metabolic processes within the biological component of this system are still elusive. Here we dissect th...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Luise Göbbels, Anja Poehlein, Albert Dumnitch, Richard Egelkamp, Cathrin Kröger, Johanna Haerdter, Thomas Hackl, Artur Feld, Horst Weller, Rolf Daniel, Wolfgang R. Streit, Marie Charlotte Schoelmerich
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/27b8e395ba3447c1b69c821bb1c04bc9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:27b8e395ba3447c1b69c821bb1c04bc9
record_format	dspace
spelling	oai:doaj.org-article:27b8e395ba3447c1b69c821bb1c04bc92021-12-02T14:16:17ZCysteine: an overlooked energy and carbon source10.1038/s41598-021-81103-z2045-2322https://doaj.org/article/27b8e395ba3447c1b69c821bb1c04bc92021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81103-zhttps://doaj.org/toc/2045-2322Abstract Biohybrids composed of microorganisms and nanoparticles have emerged as potential systems for bioenergy and high-value compound production from CO2 and light energy, yet the cellular and metabolic processes within the biological component of this system are still elusive. Here we dissect the biohybrid composed of the anaerobic acetogenic bacterium Moorella thermoacetica and cadmium sulphide nanoparticles (CdS) in terms of physiology, metabolism, enzymatics and transcriptomic profiling. Our analyses show that while the organism does not grow on l-cysteine, it is metabolized to acetate in the biohybrid system and this metabolism is independent of CdS or light. CdS cells have higher metabolic activity, despite an inhibitory effect of Cd2+ on key enzymes, because of an intracellular storage compound linked to arginine metabolism. We identify different routes how cysteine and its oxidized form can be innately metabolized by the model acetogen and what intracellular mechanisms are triggered by cysteine, cadmium or blue light.Luise GöbbelsAnja PoehleinAlbert DumnitchRichard EgelkampCathrin KrögerJohanna HaerdterThomas HacklArtur FeldHorst WellerRolf DanielWolfgang R. StreitMarie Charlotte SchoelmerichNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Luise Göbbels Anja Poehlein Albert Dumnitch Richard Egelkamp Cathrin Kröger Johanna Haerdter Thomas Hackl Artur Feld Horst Weller Rolf Daniel Wolfgang R. Streit Marie Charlotte Schoelmerich Cysteine: an overlooked energy and carbon source
description	Abstract Biohybrids composed of microorganisms and nanoparticles have emerged as potential systems for bioenergy and high-value compound production from CO2 and light energy, yet the cellular and metabolic processes within the biological component of this system are still elusive. Here we dissect the biohybrid composed of the anaerobic acetogenic bacterium Moorella thermoacetica and cadmium sulphide nanoparticles (CdS) in terms of physiology, metabolism, enzymatics and transcriptomic profiling. Our analyses show that while the organism does not grow on l-cysteine, it is metabolized to acetate in the biohybrid system and this metabolism is independent of CdS or light. CdS cells have higher metabolic activity, despite an inhibitory effect of Cd2+ on key enzymes, because of an intracellular storage compound linked to arginine metabolism. We identify different routes how cysteine and its oxidized form can be innately metabolized by the model acetogen and what intracellular mechanisms are triggered by cysteine, cadmium or blue light.
format	article
author	Luise Göbbels Anja Poehlein Albert Dumnitch Richard Egelkamp Cathrin Kröger Johanna Haerdter Thomas Hackl Artur Feld Horst Weller Rolf Daniel Wolfgang R. Streit Marie Charlotte Schoelmerich
author_facet	Luise Göbbels Anja Poehlein Albert Dumnitch Richard Egelkamp Cathrin Kröger Johanna Haerdter Thomas Hackl Artur Feld Horst Weller Rolf Daniel Wolfgang R. Streit Marie Charlotte Schoelmerich
author_sort	Luise Göbbels
title	Cysteine: an overlooked energy and carbon source
title_short	Cysteine: an overlooked energy and carbon source
title_full	Cysteine: an overlooked energy and carbon source
title_fullStr	Cysteine: an overlooked energy and carbon source
title_full_unstemmed	Cysteine: an overlooked energy and carbon source
title_sort	cysteine: an overlooked energy and carbon source
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/27b8e395ba3447c1b69c821bb1c04bc9
work_keys_str_mv	AT luisegobbels cysteineanoverlookedenergyandcarbonsource AT anjapoehlein cysteineanoverlookedenergyandcarbonsource AT albertdumnitch cysteineanoverlookedenergyandcarbonsource AT richardegelkamp cysteineanoverlookedenergyandcarbonsource AT cathrinkroger cysteineanoverlookedenergyandcarbonsource AT johannahaerdter cysteineanoverlookedenergyandcarbonsource AT thomashackl cysteineanoverlookedenergyandcarbonsource AT arturfeld cysteineanoverlookedenergyandcarbonsource AT horstweller cysteineanoverlookedenergyandcarbonsource AT rolfdaniel cysteineanoverlookedenergyandcarbonsource AT wolfgangrstreit cysteineanoverlookedenergyandcarbonsource AT mariecharlotteschoelmerich cysteineanoverlookedenergyandcarbonsource
_version_	1718391655808106496

Cysteine: an overlooked energy and carbon source

Ejemplares similares