Amelioration of indole acetic acid-induced cytotoxicity in mice using zinc nanoparticles biosynthesized with Ochradenus arabicus leaf extract

The diversity of natural phytochemicals represents an unlimited source for discovery and development of new drugs. Ochradenus arabicus, (family: Resedaceae) a notable medicinal plant displays a high content of flavonoid glycosides. This study investigates a possible preventative role of zinc nanopar...

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Autores principales:	Khalid Mashai Alanazi, Ahmed Ali Al-kawmani, Mohammad Abul Farah, Waleed Ali Hailan, Ali Alsalme, Nabil Al-Zaqri, M. Ajmal Ali, Abobakr Mahmood Almansob
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Apoptosis ROS generation DNA damage Genotoxicity Indole acetic acid Zinc nanoparticle Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/27b91d6e503443869c063392e557ec24
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spelling	oai:doaj.org-article:27b91d6e503443869c063392e557ec242021-11-20T04:56:53ZAmelioration of indole acetic acid-induced cytotoxicity in mice using zinc nanoparticles biosynthesized with Ochradenus arabicus leaf extract1319-562X10.1016/j.sjbs.2021.08.015https://doaj.org/article/27b91d6e503443869c063392e557ec242021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1319562X21006938https://doaj.org/toc/1319-562XThe diversity of natural phytochemicals represents an unlimited source for discovery and development of new drugs. Ochradenus arabicus, (family: Resedaceae) a notable medicinal plant displays a high content of flavonoid glycosides. This study investigates a possible preventative role of zinc nanoparticles biosynthesized by O. arabicus leaf extracts (OAZnO NPs) in limiting genotoxicity and cytotoxicity caused by indole acetic acid (IAA) in laboratory mice. ZnO NPs were synthesized using O. arabicus leaf extracts and characterized with UV–visible spectroscopy, scanning electron microscopy (SEM) and X-Ray diffraction (XRD). The mice were randomly distributed into the following six groups: control, OAZnO NPs treated (10 mg/kg BW), IAA treated (50 mg/kg BW); simultaneous treatment, pre-treatment, and post-treatment. Reactive oxygen species (ROS), DNA damage, chromosome aberration, and apoptosis were analyzed as toxicity endpoints. IAA exposure significantly induced production of ROS, DNA damage, apoptosis, chromosome aberrations, and micronuclei. Pre-, post-, and simultaneous treatment with OAZnO NPs ameliorated the damage caused by IAA exposure. Exposure to OAZnO NPs alone caused no toxicity for any endpoint based on comparison to controls. This study demonstrated that IAA-induced cytotoxic damage in mice could be ameliorated by treatment with OAZnO NPs. These findings require additional verification in mechanistic and in vitro studies.Khalid Mashai AlanaziAhmed Ali Al-kawmaniMohammad Abul FarahWaleed Ali HailanAli AlsalmeNabil Al-ZaqriM. Ajmal AliAbobakr Mahmood AlmansobElsevierarticleApoptosisROS generationDNA damageGenotoxicityIndole acetic acidZinc nanoparticleBiology (General)QH301-705.5ENSaudi Journal of Biological Sciences, Vol 28, Iss 12, Pp 7190-7201 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Apoptosis ROS generation DNA damage Genotoxicity Indole acetic acid Zinc nanoparticle Biology (General) QH301-705.5
spellingShingle	Apoptosis ROS generation DNA damage Genotoxicity Indole acetic acid Zinc nanoparticle Biology (General) QH301-705.5 Khalid Mashai Alanazi Ahmed Ali Al-kawmani Mohammad Abul Farah Waleed Ali Hailan Ali Alsalme Nabil Al-Zaqri M. Ajmal Ali Abobakr Mahmood Almansob Amelioration of indole acetic acid-induced cytotoxicity in mice using zinc nanoparticles biosynthesized with Ochradenus arabicus leaf extract
description	The diversity of natural phytochemicals represents an unlimited source for discovery and development of new drugs. Ochradenus arabicus, (family: Resedaceae) a notable medicinal plant displays a high content of flavonoid glycosides. This study investigates a possible preventative role of zinc nanoparticles biosynthesized by O. arabicus leaf extracts (OAZnO NPs) in limiting genotoxicity and cytotoxicity caused by indole acetic acid (IAA) in laboratory mice. ZnO NPs were synthesized using O. arabicus leaf extracts and characterized with UV–visible spectroscopy, scanning electron microscopy (SEM) and X-Ray diffraction (XRD). The mice were randomly distributed into the following six groups: control, OAZnO NPs treated (10 mg/kg BW), IAA treated (50 mg/kg BW); simultaneous treatment, pre-treatment, and post-treatment. Reactive oxygen species (ROS), DNA damage, chromosome aberration, and apoptosis were analyzed as toxicity endpoints. IAA exposure significantly induced production of ROS, DNA damage, apoptosis, chromosome aberrations, and micronuclei. Pre-, post-, and simultaneous treatment with OAZnO NPs ameliorated the damage caused by IAA exposure. Exposure to OAZnO NPs alone caused no toxicity for any endpoint based on comparison to controls. This study demonstrated that IAA-induced cytotoxic damage in mice could be ameliorated by treatment with OAZnO NPs. These findings require additional verification in mechanistic and in vitro studies.
format	article
author	Khalid Mashai Alanazi Ahmed Ali Al-kawmani Mohammad Abul Farah Waleed Ali Hailan Ali Alsalme Nabil Al-Zaqri M. Ajmal Ali Abobakr Mahmood Almansob
author_facet	Khalid Mashai Alanazi Ahmed Ali Al-kawmani Mohammad Abul Farah Waleed Ali Hailan Ali Alsalme Nabil Al-Zaqri M. Ajmal Ali Abobakr Mahmood Almansob
author_sort	Khalid Mashai Alanazi
title	Amelioration of indole acetic acid-induced cytotoxicity in mice using zinc nanoparticles biosynthesized with Ochradenus arabicus leaf extract
title_short	Amelioration of indole acetic acid-induced cytotoxicity in mice using zinc nanoparticles biosynthesized with Ochradenus arabicus leaf extract
title_full	Amelioration of indole acetic acid-induced cytotoxicity in mice using zinc nanoparticles biosynthesized with Ochradenus arabicus leaf extract
title_fullStr	Amelioration of indole acetic acid-induced cytotoxicity in mice using zinc nanoparticles biosynthesized with Ochradenus arabicus leaf extract
title_full_unstemmed	Amelioration of indole acetic acid-induced cytotoxicity in mice using zinc nanoparticles biosynthesized with Ochradenus arabicus leaf extract
title_sort	amelioration of indole acetic acid-induced cytotoxicity in mice using zinc nanoparticles biosynthesized with ochradenus arabicus leaf extract
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/27b91d6e503443869c063392e557ec24
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Amelioration of indole acetic acid-induced cytotoxicity in mice using zinc nanoparticles biosynthesized with Ochradenus arabicus leaf extract

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