Application of inter-SINE amplified polymorphism (ISAP) markers for genotyping of Cucumis melo accessions and its transferability in Coleus spp

Abstract. Sormin SYM, Purwantoro A, Setiawan AB, Teo CH. 2021. Application of inter-SINE amplified polymorphism (ISAP) markers for genotyping of Cucumis melo accessions and its transferability in Coleus spp. Biodiversitas 22: 2918-2929. An unambiguous characterization of melon (Cucumis melo L.) acce...

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Autores principales:	Siti Yuli Meilanda Sormin, Aziz Purwantoro, Agus Budi Setiawan, Chee How Teo
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2021
Materias:	coleus cucumis melo isap marker plant genotyping sine elements Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/27b98a9ab43d401c8e074fc890bb4913
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spelling	oai:doaj.org-article:27b98a9ab43d401c8e074fc890bb49132021-11-22T12:13:35ZApplication of inter-SINE amplified polymorphism (ISAP) markers for genotyping of Cucumis melo accessions and its transferability in Coleus spp1412-033X2085-472210.13057/biodiv/d220557https://doaj.org/article/27b98a9ab43d401c8e074fc890bb49132021-05-01T00:00:00Zhttps://smujo.id/biodiv/article/view/7844https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Sormin SYM, Purwantoro A, Setiawan AB, Teo CH. 2021. Application of inter-SINE amplified polymorphism (ISAP) markers for genotyping of Cucumis melo accessions and its transferability in Coleus spp. Biodiversitas 22: 2918-2929. An unambiguous characterization of melon (Cucumis melo L.) accessions based on their morphological traits is often laborious and affected by environment when compared with molecular marker genotyping. Short interspersed nuclear elements (SINEs) are highly abundant non-autonomous and non-coding retrotransposons that are widely scattered over all chromosomes of eukaryotes. They can serve as a good molecular marker for routine genotyping in plant breeding and marker-assisted selection. This study aimed to apply inter-SINE amplified polymorphism (ISAP) markers for genotyping of Cucumis melo accessions and its transferability in Coleus spp. Twenty-one C. melo accessions, one C. metuliferus E. Mey. ex Naudin, and 15 accessions of Coleus spp. were used for ISAP marker development. A copy of cucumber-specific long interspersed nuclear element (LINE) and multiple copies of melon-specific SINE were identified and isolated. ISAP primers were designed from the highly conserved region of the SINEs and LINE. The melon and cucumber-specific ISAP markers showed a higher degree of polymorphism (87.5%-100%) than potato ISAP markers (60%-100%) in all the tested melon accessions. The unweighted pair group method with arithmetic average (UPGMA) dendrogram generated from polymorphic ISAP bands clearly distinguish the Cucumis melo accessions from their distantly related wild species C. metuliferus. The transferable nature of Cucumis ISAP marker system was demonstrated in Coleus species, where the marker differentiates the tested accessions.Siti Yuli Meilanda SorminAziz PurwantoroAgus Budi SetiawanChee How TeoMBI & UNS Soloarticlecoleuscucumis meloisap markerplant genotypingsine elementsBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 5 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	coleus cucumis melo isap marker plant genotyping sine elements Biology (General) QH301-705.5
spellingShingle	coleus cucumis melo isap marker plant genotyping sine elements Biology (General) QH301-705.5 Siti Yuli Meilanda Sormin Aziz Purwantoro Agus Budi Setiawan Chee How Teo Application of inter-SINE amplified polymorphism (ISAP) markers for genotyping of Cucumis melo accessions and its transferability in Coleus spp
description	Abstract. Sormin SYM, Purwantoro A, Setiawan AB, Teo CH. 2021. Application of inter-SINE amplified polymorphism (ISAP) markers for genotyping of Cucumis melo accessions and its transferability in Coleus spp. Biodiversitas 22: 2918-2929. An unambiguous characterization of melon (Cucumis melo L.) accessions based on their morphological traits is often laborious and affected by environment when compared with molecular marker genotyping. Short interspersed nuclear elements (SINEs) are highly abundant non-autonomous and non-coding retrotransposons that are widely scattered over all chromosomes of eukaryotes. They can serve as a good molecular marker for routine genotyping in plant breeding and marker-assisted selection. This study aimed to apply inter-SINE amplified polymorphism (ISAP) markers for genotyping of Cucumis melo accessions and its transferability in Coleus spp. Twenty-one C. melo accessions, one C. metuliferus E. Mey. ex Naudin, and 15 accessions of Coleus spp. were used for ISAP marker development. A copy of cucumber-specific long interspersed nuclear element (LINE) and multiple copies of melon-specific SINE were identified and isolated. ISAP primers were designed from the highly conserved region of the SINEs and LINE. The melon and cucumber-specific ISAP markers showed a higher degree of polymorphism (87.5%-100%) than potato ISAP markers (60%-100%) in all the tested melon accessions. The unweighted pair group method with arithmetic average (UPGMA) dendrogram generated from polymorphic ISAP bands clearly distinguish the Cucumis melo accessions from their distantly related wild species C. metuliferus. The transferable nature of Cucumis ISAP marker system was demonstrated in Coleus species, where the marker differentiates the tested accessions.
format	article
author	Siti Yuli Meilanda Sormin Aziz Purwantoro Agus Budi Setiawan Chee How Teo
author_facet	Siti Yuli Meilanda Sormin Aziz Purwantoro Agus Budi Setiawan Chee How Teo
author_sort	Siti Yuli Meilanda Sormin
title	Application of inter-SINE amplified polymorphism (ISAP) markers for genotyping of Cucumis melo accessions and its transferability in Coleus spp
title_short	Application of inter-SINE amplified polymorphism (ISAP) markers for genotyping of Cucumis melo accessions and its transferability in Coleus spp
title_full	Application of inter-SINE amplified polymorphism (ISAP) markers for genotyping of Cucumis melo accessions and its transferability in Coleus spp
title_fullStr	Application of inter-SINE amplified polymorphism (ISAP) markers for genotyping of Cucumis melo accessions and its transferability in Coleus spp
title_full_unstemmed	Application of inter-SINE amplified polymorphism (ISAP) markers for genotyping of Cucumis melo accessions and its transferability in Coleus spp
title_sort	application of inter-sine amplified polymorphism (isap) markers for genotyping of cucumis melo accessions and its transferability in coleus spp
publisher	MBI & UNS Solo
publishDate	2021
url	https://doaj.org/article/27b98a9ab43d401c8e074fc890bb4913
work_keys_str_mv	AT sitiyulimeilandasormin applicationofintersineamplifiedpolymorphismisapmarkersforgenotypingofcucumismeloaccessionsanditstransferabilityincoleusspp AT azizpurwantoro applicationofintersineamplifiedpolymorphismisapmarkersforgenotypingofcucumismeloaccessionsanditstransferabilityincoleusspp AT agusbudisetiawan applicationofintersineamplifiedpolymorphismisapmarkersforgenotypingofcucumismeloaccessionsanditstransferabilityincoleusspp AT cheehowteo applicationofintersineamplifiedpolymorphismisapmarkersforgenotypingofcucumismeloaccessionsanditstransferabilityincoleusspp
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Application of inter-SINE amplified polymorphism (ISAP) markers for genotyping of Cucumis melo accessions and its transferability in Coleus spp

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