Effect of Berberis vulgaris L. root extract on ifosfamide-induced in vivo toxicity and in vitro cytotoxicity

Abstract Ifosfamide is a widely used chemotherapeutic agent having broad-spectrum efficacy against several tumors. However, nephro, hepato, neuro cardio, and hematological toxicities associated with ifosfamide render its use limited. These side effects could range from organ failure to life-threaten...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shazia Ilyas, Raheela Tabasum, Ali Iftikhar, Mamoona Nazir, Amina Hussain, Aroosha Hussain, Muhammad Sajjad Ali, Farooq Saleem, Uzma Saleem, Matheus Froeyen, Iskandar Abdullah, Muhammad Usman Mirza, Sarfraz Ahmad
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/27c6a089228c42ba9b88e2326f6f79ce
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:27c6a089228c42ba9b88e2326f6f79ce
record_format	dspace
spelling	oai:doaj.org-article:27c6a089228c42ba9b88e2326f6f79ce2021-12-02T15:23:28ZEffect of Berberis vulgaris L. root extract on ifosfamide-induced in vivo toxicity and in vitro cytotoxicity10.1038/s41598-020-80579-52045-2322https://doaj.org/article/27c6a089228c42ba9b88e2326f6f79ce2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80579-5https://doaj.org/toc/2045-2322Abstract Ifosfamide is a widely used chemotherapeutic agent having broad-spectrum efficacy against several tumors. However, nephro, hepato, neuro cardio, and hematological toxicities associated with ifosfamide render its use limited. These side effects could range from organ failure to life-threatening situations. The present study aimed to evaluate the attenuating efficiency of Berberis vulgaris root extract (BvRE), a potent nephroprotective, hepatoprotective, and lipid-lowering agent, against ifosfamide-induced toxicities. The study design comprised eight groups of Swiss albino rats to assess different dose regimes of BvRE and ifosfamide. Biochemical analysis of serum (serum albumin, blood urea nitrogen, creatinine, alanine transaminase, aspartate transaminase, alkaline phosphatase, lactate dehydrogenase, total cholesterol, and triglycerides) along with complete blood count was performed. Kidney, liver, brain, and heart tissue homogenates were used to find malondialdehyde, catalase, and glutathione S-transferase levels in addition to the acetylcholinesterase of brain tissue. The results were further validated with the help of the histopathology of the selected organs. HeLa cells were used to assess the effect of BvRE on ifosfamide cytotoxicity in MTT assay. The results revealed that pre- and post-treatment regimens of BvRE, as well as the combination therapy exhibited marked protective effects against ifosfamide-induced nephro, hepato, neuro, and cardiotoxicity. Moreover, ifosfamide depicted a synergistic in vitro cytotoxic effect on HeLa cells in the presence of BvRE. These results corroborate that the combination therapy of ifosfamide with BvRE in cancer treatment can potentiate the anticancer effects of ifosfamide along with the amelioration of its conspicuous side effects.Shazia IlyasRaheela TabasumAli IftikharMamoona NazirAmina HussainAroosha HussainMuhammad Sajjad AliFarooq SaleemUzma SaleemMatheus FroeyenIskandar AbdullahMuhammad Usman MirzaSarfraz AhmadNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shazia Ilyas Raheela Tabasum Ali Iftikhar Mamoona Nazir Amina Hussain Aroosha Hussain Muhammad Sajjad Ali Farooq Saleem Uzma Saleem Matheus Froeyen Iskandar Abdullah Muhammad Usman Mirza Sarfraz Ahmad Effect of Berberis vulgaris L. root extract on ifosfamide-induced in vivo toxicity and in vitro cytotoxicity
description	Abstract Ifosfamide is a widely used chemotherapeutic agent having broad-spectrum efficacy against several tumors. However, nephro, hepato, neuro cardio, and hematological toxicities associated with ifosfamide render its use limited. These side effects could range from organ failure to life-threatening situations. The present study aimed to evaluate the attenuating efficiency of Berberis vulgaris root extract (BvRE), a potent nephroprotective, hepatoprotective, and lipid-lowering agent, against ifosfamide-induced toxicities. The study design comprised eight groups of Swiss albino rats to assess different dose regimes of BvRE and ifosfamide. Biochemical analysis of serum (serum albumin, blood urea nitrogen, creatinine, alanine transaminase, aspartate transaminase, alkaline phosphatase, lactate dehydrogenase, total cholesterol, and triglycerides) along with complete blood count was performed. Kidney, liver, brain, and heart tissue homogenates were used to find malondialdehyde, catalase, and glutathione S-transferase levels in addition to the acetylcholinesterase of brain tissue. The results were further validated with the help of the histopathology of the selected organs. HeLa cells were used to assess the effect of BvRE on ifosfamide cytotoxicity in MTT assay. The results revealed that pre- and post-treatment regimens of BvRE, as well as the combination therapy exhibited marked protective effects against ifosfamide-induced nephro, hepato, neuro, and cardiotoxicity. Moreover, ifosfamide depicted a synergistic in vitro cytotoxic effect on HeLa cells in the presence of BvRE. These results corroborate that the combination therapy of ifosfamide with BvRE in cancer treatment can potentiate the anticancer effects of ifosfamide along with the amelioration of its conspicuous side effects.
format	article
author	Shazia Ilyas Raheela Tabasum Ali Iftikhar Mamoona Nazir Amina Hussain Aroosha Hussain Muhammad Sajjad Ali Farooq Saleem Uzma Saleem Matheus Froeyen Iskandar Abdullah Muhammad Usman Mirza Sarfraz Ahmad
author_facet	Shazia Ilyas Raheela Tabasum Ali Iftikhar Mamoona Nazir Amina Hussain Aroosha Hussain Muhammad Sajjad Ali Farooq Saleem Uzma Saleem Matheus Froeyen Iskandar Abdullah Muhammad Usman Mirza Sarfraz Ahmad
author_sort	Shazia Ilyas
title	Effect of Berberis vulgaris L. root extract on ifosfamide-induced in vivo toxicity and in vitro cytotoxicity
title_short	Effect of Berberis vulgaris L. root extract on ifosfamide-induced in vivo toxicity and in vitro cytotoxicity
title_full	Effect of Berberis vulgaris L. root extract on ifosfamide-induced in vivo toxicity and in vitro cytotoxicity
title_fullStr	Effect of Berberis vulgaris L. root extract on ifosfamide-induced in vivo toxicity and in vitro cytotoxicity
title_full_unstemmed	Effect of Berberis vulgaris L. root extract on ifosfamide-induced in vivo toxicity and in vitro cytotoxicity
title_sort	effect of berberis vulgaris l. root extract on ifosfamide-induced in vivo toxicity and in vitro cytotoxicity
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/27c6a089228c42ba9b88e2326f6f79ce
work_keys_str_mv	AT shaziailyas effectofberberisvulgarislrootextractonifosfamideinducedinvivotoxicityandinvitrocytotoxicity AT raheelatabasum effectofberberisvulgarislrootextractonifosfamideinducedinvivotoxicityandinvitrocytotoxicity AT aliiftikhar effectofberberisvulgarislrootextractonifosfamideinducedinvivotoxicityandinvitrocytotoxicity AT mamoonanazir effectofberberisvulgarislrootextractonifosfamideinducedinvivotoxicityandinvitrocytotoxicity AT aminahussain effectofberberisvulgarislrootextractonifosfamideinducedinvivotoxicityandinvitrocytotoxicity AT arooshahussain effectofberberisvulgarislrootextractonifosfamideinducedinvivotoxicityandinvitrocytotoxicity AT muhammadsajjadali effectofberberisvulgarislrootextractonifosfamideinducedinvivotoxicityandinvitrocytotoxicity AT farooqsaleem effectofberberisvulgarislrootextractonifosfamideinducedinvivotoxicityandinvitrocytotoxicity AT uzmasaleem effectofberberisvulgarislrootextractonifosfamideinducedinvivotoxicityandinvitrocytotoxicity AT matheusfroeyen effectofberberisvulgarislrootextractonifosfamideinducedinvivotoxicityandinvitrocytotoxicity AT iskandarabdullah effectofberberisvulgarislrootextractonifosfamideinducedinvivotoxicityandinvitrocytotoxicity AT muhammadusmanmirza effectofberberisvulgarislrootextractonifosfamideinducedinvivotoxicityandinvitrocytotoxicity AT sarfrazahmad effectofberberisvulgarislrootextractonifosfamideinducedinvivotoxicityandinvitrocytotoxicity
_version_	1718387251663077376

Effect of Berberis vulgaris L. root extract on ifosfamide-induced in vivo toxicity and in vitro cytotoxicity

Ejemplares similares