Characteristics of Methicillin-Resistant Staphylococcus aureus (MRSA) and Methicillin Sensitive Staphylococcus aureus (MSSA) and their inhibitory response by ethanol extract of Abrus precatorius

Abstract. Mutmainnah BQ, Baktir A, Ni’matuzahroh. 2020. Characteristics of Methicillin-Resistant Staphylococcus aureus (MRSA) and Methicillin Sensitive Staphylococcus aureus (MSSA) and their inhibitory response by ethanol extract of Abrus precatorius. Biodiversitas 21: 4076-4085. Three isolates of S...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	BQ. MUTMAINNAH, AFAF BAKTIR, NI’MATUZAHROH NI’MATUZAHROH
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	mrsa mssa ethanol extract mrsa, mssa, ethanol extract, abrus precatorius Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/27cf204127224c96a27cfebc84c9d8c4
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:27cf204127224c96a27cfebc84c9d8c4
record_format	dspace
spelling	oai:doaj.org-article:27cf204127224c96a27cfebc84c9d8c42021-11-22T00:41:43ZCharacteristics of Methicillin-Resistant Staphylococcus aureus (MRSA) and Methicillin Sensitive Staphylococcus aureus (MSSA) and their inhibitory response by ethanol extract of Abrus precatorius1412-033X2085-472210.13057/biodiv/d210919https://doaj.org/article/27cf204127224c96a27cfebc84c9d8c42020-08-01T00:00:00Zhttps://smujo.id/biodiv/article/view/5919https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Mutmainnah BQ, Baktir A, Ni’matuzahroh. 2020. Characteristics of Methicillin-Resistant Staphylococcus aureus (MRSA) and Methicillin Sensitive Staphylococcus aureus (MSSA) and their inhibitory response by ethanol extract of Abrus precatorius. Biodiversitas 21: 4076-4085. Three isolates of Staphylococcus bacteria with the code MRSA 22372, MSSA 22187 and MSSA 22366 originated from the urine of the patient at Dr. Regional General Hospital Soetomo, Clinical Microbiology Installation Surabaya, Indonesia. Differences in bacterial strains affect their sensitivity to antimicrobial agents. The active ingredient of the ethanol extract of the leaves of Abrus precatorius L. has the potential to inhibit bacterial growth. This study aims to further characterize the bacteria MRSA 22372, MSSA 22187 and MSSA 22366 based on morphological, biochemical and molecular characters and to compare the growth inhibitory response of these three bacteria due to the treatment of variations in the ethanol extract of A. precatorius of 25 mgL-1- 800 mgL-1 leaves. The results showed there were differences in the diameter of bacterial colonies, the ability to ferment glucose and sucrose, and the production of urease and catalase. The molecular characteristics of the three bacteria have no similarity in the order of nucleotide bases or phylogenetic proximity to each other. Ethanol extract of A. precatorius leaves at a concentration of 800 mgL-1 inhibited the growth of MSSA 22187 with an inhibition zone of 41 mm and decreased the MSSA 22366 growth by 67.6%. MIC value of ethanol extract of A. precatorius leaves in all three bacteria was 25 mgL-1 with growth inhibition up to 29.4%, 35.3%, and 29.4% respectively. Keyword : MRSA, MSSA, ethanol extract, Abrus precatorius L.BQ. MUTMAINNAHAFAF BAKTIRNI’MATUZAHROH NI’MATUZAHROHMBI & UNS Soloarticlemrsamssaethanol extractmrsa, mssa, ethanol extract, abrus precatoriusBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 9 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	mrsa mssa ethanol extract mrsa, mssa, ethanol extract, abrus precatorius Biology (General) QH301-705.5
spellingShingle	mrsa mssa ethanol extract mrsa, mssa, ethanol extract, abrus precatorius Biology (General) QH301-705.5 BQ. MUTMAINNAH AFAF BAKTIR NI’MATUZAHROH NI’MATUZAHROH Characteristics of Methicillin-Resistant Staphylococcus aureus (MRSA) and Methicillin Sensitive Staphylococcus aureus (MSSA) and their inhibitory response by ethanol extract of Abrus precatorius
description	Abstract. Mutmainnah BQ, Baktir A, Ni’matuzahroh. 2020. Characteristics of Methicillin-Resistant Staphylococcus aureus (MRSA) and Methicillin Sensitive Staphylococcus aureus (MSSA) and their inhibitory response by ethanol extract of Abrus precatorius. Biodiversitas 21: 4076-4085. Three isolates of Staphylococcus bacteria with the code MRSA 22372, MSSA 22187 and MSSA 22366 originated from the urine of the patient at Dr. Regional General Hospital Soetomo, Clinical Microbiology Installation Surabaya, Indonesia. Differences in bacterial strains affect their sensitivity to antimicrobial agents. The active ingredient of the ethanol extract of the leaves of Abrus precatorius L. has the potential to inhibit bacterial growth. This study aims to further characterize the bacteria MRSA 22372, MSSA 22187 and MSSA 22366 based on morphological, biochemical and molecular characters and to compare the growth inhibitory response of these three bacteria due to the treatment of variations in the ethanol extract of A. precatorius of 25 mgL-1- 800 mgL-1 leaves. The results showed there were differences in the diameter of bacterial colonies, the ability to ferment glucose and sucrose, and the production of urease and catalase. The molecular characteristics of the three bacteria have no similarity in the order of nucleotide bases or phylogenetic proximity to each other. Ethanol extract of A. precatorius leaves at a concentration of 800 mgL-1 inhibited the growth of MSSA 22187 with an inhibition zone of 41 mm and decreased the MSSA 22366 growth by 67.6%. MIC value of ethanol extract of A. precatorius leaves in all three bacteria was 25 mgL-1 with growth inhibition up to 29.4%, 35.3%, and 29.4% respectively. Keyword : MRSA, MSSA, ethanol extract, Abrus precatorius L.
format	article
author	BQ. MUTMAINNAH AFAF BAKTIR NI’MATUZAHROH NI’MATUZAHROH
author_facet	BQ. MUTMAINNAH AFAF BAKTIR NI’MATUZAHROH NI’MATUZAHROH
author_sort	BQ. MUTMAINNAH
title	Characteristics of Methicillin-Resistant Staphylococcus aureus (MRSA) and Methicillin Sensitive Staphylococcus aureus (MSSA) and their inhibitory response by ethanol extract of Abrus precatorius
title_short	Characteristics of Methicillin-Resistant Staphylococcus aureus (MRSA) and Methicillin Sensitive Staphylococcus aureus (MSSA) and their inhibitory response by ethanol extract of Abrus precatorius
title_full	Characteristics of Methicillin-Resistant Staphylococcus aureus (MRSA) and Methicillin Sensitive Staphylococcus aureus (MSSA) and their inhibitory response by ethanol extract of Abrus precatorius
title_fullStr	Characteristics of Methicillin-Resistant Staphylococcus aureus (MRSA) and Methicillin Sensitive Staphylococcus aureus (MSSA) and their inhibitory response by ethanol extract of Abrus precatorius
title_full_unstemmed	Characteristics of Methicillin-Resistant Staphylococcus aureus (MRSA) and Methicillin Sensitive Staphylococcus aureus (MSSA) and their inhibitory response by ethanol extract of Abrus precatorius
title_sort	characteristics of methicillin-resistant staphylococcus aureus (mrsa) and methicillin sensitive staphylococcus aureus (mssa) and their inhibitory response by ethanol extract of abrus precatorius
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/27cf204127224c96a27cfebc84c9d8c4
work_keys_str_mv	AT bqmutmainnah characteristicsofmethicillinresistantstaphylococcusaureusmrsaandmethicillinsensitivestaphylococcusaureusmssaandtheirinhibitoryresponsebyethanolextractofabrusprecatorius AT afafbaktir characteristicsofmethicillinresistantstaphylococcusaureusmrsaandmethicillinsensitivestaphylococcusaureusmssaandtheirinhibitoryresponsebyethanolextractofabrusprecatorius AT nimatuzahrohnimatuzahroh characteristicsofmethicillinresistantstaphylococcusaureusmrsaandmethicillinsensitivestaphylococcusaureusmssaandtheirinhibitoryresponsebyethanolextractofabrusprecatorius
_version_	1718418510555643904

Characteristics of Methicillin-Resistant Staphylococcus aureus (MRSA) and Methicillin Sensitive Staphylococcus aureus (MSSA) and their inhibitory response by ethanol extract of Abrus precatorius

Ejemplares similares