PCR-based molecular characterization of Toxocara spp. using feces of stray cats: a study from Southwest Iran.

Feces of stray cat are potential sources of gastrointestinal parasites and play a crucial role in spreading and transmitting parasite eggs, larvae, and oocysts through contamination of soil, food, or water. In this study, we investigated the prevalence of Toxocara spp. infection in stray cats in Ahv...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Shahram Khademvatan, Fakher Rahim, Mahdi Tavalla, Rahman Abdizadeh, Mahmoud Hashemitabar
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/27d1cb33bc264efb90219cfff7eb95aa
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:27d1cb33bc264efb90219cfff7eb95aa
record_format	dspace
spelling	oai:doaj.org-article:27d1cb33bc264efb90219cfff7eb95aa2021-11-18T07:43:13ZPCR-based molecular characterization of Toxocara spp. using feces of stray cats: a study from Southwest Iran.1932-620310.1371/journal.pone.0065293https://doaj.org/article/27d1cb33bc264efb90219cfff7eb95aa2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23755213/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Feces of stray cat are potential sources of gastrointestinal parasites and play a crucial role in spreading and transmitting parasite eggs, larvae, and oocysts through contamination of soil, food, or water. In this study, we investigated the prevalence of Toxocara spp. infection in stray cats in Ahvaz city, southwest Iran. Eggs of Toxocara spp. in feces of stray cats were detected by the sucrose flotation method, and identification was conducted by polymerase chain reaction (PCR) and DNA sequencing. Of the 140 fecal samples that were randomly collected from public environments during the months of January to May 2012, 45% were found to harbour Toxocara spp. eggs. The highest prevalence of Toxocara spp. eggs was found in the central area of Ahvaz city (28.6%). T. canis eggs were found in 4 (6.34%) of the 63 positive samples. Stray cats are found in parks, playgrounds, and other public places and may be a potential contamination risk. Identification of Toxocara spp. using molecular methods is sufficiently sensitive to detect low levels of parasites and identify the different Toxocara spp. in feces. The relatively high prevalence of Toxocara spp. infection may continue to increase due to lack of effective environmental hygiene control in Iran. Consequently, there is a need to plan adequate programs to detect, identify, and control this infection as well as stray cats in the region.Shahram KhademvatanFakher RahimMahdi TavallaRahman AbdizadehMahmoud HashemitabarPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 6, p e65293 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shahram Khademvatan Fakher Rahim Mahdi Tavalla Rahman Abdizadeh Mahmoud Hashemitabar PCR-based molecular characterization of Toxocara spp. using feces of stray cats: a study from Southwest Iran.
description	Feces of stray cat are potential sources of gastrointestinal parasites and play a crucial role in spreading and transmitting parasite eggs, larvae, and oocysts through contamination of soil, food, or water. In this study, we investigated the prevalence of Toxocara spp. infection in stray cats in Ahvaz city, southwest Iran. Eggs of Toxocara spp. in feces of stray cats were detected by the sucrose flotation method, and identification was conducted by polymerase chain reaction (PCR) and DNA sequencing. Of the 140 fecal samples that were randomly collected from public environments during the months of January to May 2012, 45% were found to harbour Toxocara spp. eggs. The highest prevalence of Toxocara spp. eggs was found in the central area of Ahvaz city (28.6%). T. canis eggs were found in 4 (6.34%) of the 63 positive samples. Stray cats are found in parks, playgrounds, and other public places and may be a potential contamination risk. Identification of Toxocara spp. using molecular methods is sufficiently sensitive to detect low levels of parasites and identify the different Toxocara spp. in feces. The relatively high prevalence of Toxocara spp. infection may continue to increase due to lack of effective environmental hygiene control in Iran. Consequently, there is a need to plan adequate programs to detect, identify, and control this infection as well as stray cats in the region.
format	article
author	Shahram Khademvatan Fakher Rahim Mahdi Tavalla Rahman Abdizadeh Mahmoud Hashemitabar
author_facet	Shahram Khademvatan Fakher Rahim Mahdi Tavalla Rahman Abdizadeh Mahmoud Hashemitabar
author_sort	Shahram Khademvatan
title	PCR-based molecular characterization of Toxocara spp. using feces of stray cats: a study from Southwest Iran.
title_short	PCR-based molecular characterization of Toxocara spp. using feces of stray cats: a study from Southwest Iran.
title_full	PCR-based molecular characterization of Toxocara spp. using feces of stray cats: a study from Southwest Iran.
title_fullStr	PCR-based molecular characterization of Toxocara spp. using feces of stray cats: a study from Southwest Iran.
title_full_unstemmed	PCR-based molecular characterization of Toxocara spp. using feces of stray cats: a study from Southwest Iran.
title_sort	pcr-based molecular characterization of toxocara spp. using feces of stray cats: a study from southwest iran.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/27d1cb33bc264efb90219cfff7eb95aa
work_keys_str_mv	AT shahramkhademvatan pcrbasedmolecularcharacterizationoftoxocarasppusingfecesofstraycatsastudyfromsouthwestiran AT fakherrahim pcrbasedmolecularcharacterizationoftoxocarasppusingfecesofstraycatsastudyfromsouthwestiran AT mahditavalla pcrbasedmolecularcharacterizationoftoxocarasppusingfecesofstraycatsastudyfromsouthwestiran AT rahmanabdizadeh pcrbasedmolecularcharacterizationoftoxocarasppusingfecesofstraycatsastudyfromsouthwestiran AT mahmoudhashemitabar pcrbasedmolecularcharacterizationoftoxocarasppusingfecesofstraycatsastudyfromsouthwestiran
_version_	1718423023053176832

PCR-based molecular characterization of Toxocara spp. using feces of stray cats: a study from Southwest Iran.

Ejemplares similares