$Combined Protein and Alkaloid Research of Chelidonium majus Latex Reveals CmMLP1 Accompanied by Alkaloids with Cytotoxic Potential to Human Cervical Carcinoma Cells$
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Combined Protein and Alkaloid Research of Chelidonium majus Latex Reveals CmMLP1 Accompanied by Alkaloids with Cytotoxic Potential to Human Cervical Carcinoma Cells

Chelidonium majus L. is a latex-bearing plant used in traditional folk medicine to treat human papillomavirus (HPV)-caused warts, papillae, and condylomas. Its latex and extracts are rich in many low-molecular compounds and proteins, but there is little or no information on their...

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Autores principales:	Robert Nawrot, Alicja Warowicka, Piotr Józef Rudzki, Oskar Musidlak, Katarzyna Magdalena Dolata, Jacek Musijowski, Elżbieta Urszula Stolarczyk, Anna Goździcka-Józefiak
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	major latex protein <i>Chelidonium majus</i> greater celandine defense-related proteins alkaloids molecular docking Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/27d8980aa3084ded9adde44c1a93aa48
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spelling	oai:doaj.org-article:27d8980aa3084ded9adde44c1a93aa482021-11-11T17:16:10ZCombined Protein and Alkaloid Research of <i>Chelidonium majus</i> Latex Reveals CmMLP1 Accompanied by Alkaloids with Cytotoxic Potential to Human Cervical Carcinoma Cells10.3390/ijms2221118381422-00671661-6596https://doaj.org/article/27d8980aa3084ded9adde44c1a93aa482021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11838https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067<i>Chelidonium majus</i> L. is a latex-bearing plant used in traditional folk medicine to treat human papillomavirus (HPV)-caused warts, papillae, and condylomas. Its latex and extracts are rich in many low-molecular compounds and proteins, but there is little or no information on their potential interaction. We describe the isolation and identification of a novel major latex protein (CmMLP1) composed of 147 amino acids and present a model of its structure containing a conserved hydrophobic cavity with high affinity to berberine, 8-hydroxycheleritrine, and dihydroberberine. CmMLP1 and the accompanying three alkaloids were present in the eluted chromatographic fractions of latex. They decreased in vitro viability of human cervical cancer cells (HPV-negative and HPV-positive). We combined, for the first time, research on macromolecular and low-molecular-weight compounds of latex-bearing plants in contrast to other studies that investigated proteins and alkaloids separately. The observed interaction between latex protein and alkaloids may influence our knowledge on plant defense. The proposed toolbox may help in further understanding of plant disease resistance and in pharmacological research.Robert NawrotAlicja WarowickaPiotr Józef RudzkiOskar MusidlakKatarzyna Magdalena DolataJacek MusijowskiElżbieta Urszula StolarczykAnna Goździcka-JózefiakMDPI AGarticlemajor latex protein<i>Chelidonium majus</i>greater celandinedefense-related proteinsalkaloidsmolecular dockingBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11838, p 11838 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	major latex protein <i>Chelidonium majus</i> greater celandine defense-related proteins alkaloids molecular docking Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	major latex protein <i>Chelidonium majus</i> greater celandine defense-related proteins alkaloids molecular docking Biology (General) QH301-705.5 Chemistry QD1-999 Robert Nawrot Alicja Warowicka Piotr Józef Rudzki Oskar Musidlak Katarzyna Magdalena Dolata Jacek Musijowski Elżbieta Urszula Stolarczyk Anna Goździcka-Józefiak Combined Protein and Alkaloid Research of <i>Chelidonium majus</i> Latex Reveals CmMLP1 Accompanied by Alkaloids with Cytotoxic Potential to Human Cervical Carcinoma Cells
description	<i>Chelidonium majus</i> L. is a latex-bearing plant used in traditional folk medicine to treat human papillomavirus (HPV)-caused warts, papillae, and condylomas. Its latex and extracts are rich in many low-molecular compounds and proteins, but there is little or no information on their potential interaction. We describe the isolation and identification of a novel major latex protein (CmMLP1) composed of 147 amino acids and present a model of its structure containing a conserved hydrophobic cavity with high affinity to berberine, 8-hydroxycheleritrine, and dihydroberberine. CmMLP1 and the accompanying three alkaloids were present in the eluted chromatographic fractions of latex. They decreased in vitro viability of human cervical cancer cells (HPV-negative and HPV-positive). We combined, for the first time, research on macromolecular and low-molecular-weight compounds of latex-bearing plants in contrast to other studies that investigated proteins and alkaloids separately. The observed interaction between latex protein and alkaloids may influence our knowledge on plant defense. The proposed toolbox may help in further understanding of plant disease resistance and in pharmacological research.
format	article
author	Robert Nawrot Alicja Warowicka Piotr Józef Rudzki Oskar Musidlak Katarzyna Magdalena Dolata Jacek Musijowski Elżbieta Urszula Stolarczyk Anna Goździcka-Józefiak
author_facet	Robert Nawrot Alicja Warowicka Piotr Józef Rudzki Oskar Musidlak Katarzyna Magdalena Dolata Jacek Musijowski Elżbieta Urszula Stolarczyk Anna Goździcka-Józefiak
author_sort	Robert Nawrot
title	Combined Protein and Alkaloid Research of <i>Chelidonium majus</i> Latex Reveals CmMLP1 Accompanied by Alkaloids with Cytotoxic Potential to Human Cervical Carcinoma Cells
title_short	Combined Protein and Alkaloid Research of <i>Chelidonium majus</i> Latex Reveals CmMLP1 Accompanied by Alkaloids with Cytotoxic Potential to Human Cervical Carcinoma Cells
title_full	Combined Protein and Alkaloid Research of <i>Chelidonium majus</i> Latex Reveals CmMLP1 Accompanied by Alkaloids with Cytotoxic Potential to Human Cervical Carcinoma Cells
title_fullStr	Combined Protein and Alkaloid Research of <i>Chelidonium majus</i> Latex Reveals CmMLP1 Accompanied by Alkaloids with Cytotoxic Potential to Human Cervical Carcinoma Cells
title_full_unstemmed	Combined Protein and Alkaloid Research of <i>Chelidonium majus</i> Latex Reveals CmMLP1 Accompanied by Alkaloids with Cytotoxic Potential to Human Cervical Carcinoma Cells
title_sort	combined protein and alkaloid research of <i>chelidonium majus</i> latex reveals cmmlp1 accompanied by alkaloids with cytotoxic potential to human cervical carcinoma cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/27d8980aa3084ded9adde44c1a93aa48
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Combined Protein and Alkaloid Research of <i>Chelidonium majus</i> Latex Reveals CmMLP1 Accompanied by Alkaloids with Cytotoxic Potential to Human Cervical Carcinoma Cells

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