High-throughput quantitation of SARS-CoV-2 antibodies in a single-dilution homogeneous assay

Abstract SARS-CoV-2 emerged in late 2019 and has since spread around the world, causing a pandemic of the respiratory disease COVID-19. Detecting antibodies against the virus is an essential tool for tracking infections and developing vaccines. Such tests, primarily utilizing the enzyme-linked immun...

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Autores principales:	Markus H. Kainulainen, Eric Bergeron, Payel Chatterjee, Asheley P. Chapman, Joo Lee, Asiya Chida, Xiaoling Tang, Rebekah E. Wharton, Kristina B. Mercer, Marla Petway, Harley M. Jenks, Timothy D. Flietstra, Amy J. Schuh, Panayampalli S. Satheshkumar, Jasmine M. Chaitram, S. Michele Owen, Laura K. McMullan, Mike Flint, M. G. Finn, Jason M. Goldstein, Joel M. Montgomery, Christina F. Spiropoulou
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/27dfd414a1384a84aa5cb8b2992de9c1
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spelling	oai:doaj.org-article:27dfd414a1384a84aa5cb8b2992de9c12021-12-02T17:30:46ZHigh-throughput quantitation of SARS-CoV-2 antibodies in a single-dilution homogeneous assay10.1038/s41598-021-91300-52045-2322https://doaj.org/article/27dfd414a1384a84aa5cb8b2992de9c12021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91300-5https://doaj.org/toc/2045-2322Abstract SARS-CoV-2 emerged in late 2019 and has since spread around the world, causing a pandemic of the respiratory disease COVID-19. Detecting antibodies against the virus is an essential tool for tracking infections and developing vaccines. Such tests, primarily utilizing the enzyme-linked immunosorbent assay (ELISA) principle, can be either qualitative (reporting positive/negative results) or quantitative (reporting a value representing the quantity of specific antibodies). Quantitation is vital for determining stability or decline of antibody titers in convalescence, efficacy of different vaccination regimens, and detection of asymptomatic infections. Quantitation typically requires two-step ELISA testing, in which samples are first screened in a qualitative assay and positive samples are subsequently analyzed as a dilution series. To overcome the throughput limitations of this approach, we developed a simpler and faster system that is highly automatable and achieves quantitation in a single-dilution screening format with sensitivity and specificity comparable to those of ELISA.Markus H. KainulainenEric BergeronPayel ChatterjeeAsheley P. ChapmanJoo LeeAsiya ChidaXiaoling TangRebekah E. WhartonKristina B. MercerMarla PetwayHarley M. JenksTimothy D. FlietstraAmy J. SchuhPanayampalli S. SatheshkumarJasmine M. ChaitramS. Michele OwenLaura K. McMullanMike FlintM. G. FinnJason M. GoldsteinJoel M. MontgomeryChristina F. SpiropoulouNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Markus H. Kainulainen Eric Bergeron Payel Chatterjee Asheley P. Chapman Joo Lee Asiya Chida Xiaoling Tang Rebekah E. Wharton Kristina B. Mercer Marla Petway Harley M. Jenks Timothy D. Flietstra Amy J. Schuh Panayampalli S. Satheshkumar Jasmine M. Chaitram S. Michele Owen Laura K. McMullan Mike Flint M. G. Finn Jason M. Goldstein Joel M. Montgomery Christina F. Spiropoulou High-throughput quantitation of SARS-CoV-2 antibodies in a single-dilution homogeneous assay
description	Abstract SARS-CoV-2 emerged in late 2019 and has since spread around the world, causing a pandemic of the respiratory disease COVID-19. Detecting antibodies against the virus is an essential tool for tracking infections and developing vaccines. Such tests, primarily utilizing the enzyme-linked immunosorbent assay (ELISA) principle, can be either qualitative (reporting positive/negative results) or quantitative (reporting a value representing the quantity of specific antibodies). Quantitation is vital for determining stability or decline of antibody titers in convalescence, efficacy of different vaccination regimens, and detection of asymptomatic infections. Quantitation typically requires two-step ELISA testing, in which samples are first screened in a qualitative assay and positive samples are subsequently analyzed as a dilution series. To overcome the throughput limitations of this approach, we developed a simpler and faster system that is highly automatable and achieves quantitation in a single-dilution screening format with sensitivity and specificity comparable to those of ELISA.
format	article
author	Markus H. Kainulainen Eric Bergeron Payel Chatterjee Asheley P. Chapman Joo Lee Asiya Chida Xiaoling Tang Rebekah E. Wharton Kristina B. Mercer Marla Petway Harley M. Jenks Timothy D. Flietstra Amy J. Schuh Panayampalli S. Satheshkumar Jasmine M. Chaitram S. Michele Owen Laura K. McMullan Mike Flint M. G. Finn Jason M. Goldstein Joel M. Montgomery Christina F. Spiropoulou
author_facet	Markus H. Kainulainen Eric Bergeron Payel Chatterjee Asheley P. Chapman Joo Lee Asiya Chida Xiaoling Tang Rebekah E. Wharton Kristina B. Mercer Marla Petway Harley M. Jenks Timothy D. Flietstra Amy J. Schuh Panayampalli S. Satheshkumar Jasmine M. Chaitram S. Michele Owen Laura K. McMullan Mike Flint M. G. Finn Jason M. Goldstein Joel M. Montgomery Christina F. Spiropoulou
author_sort	Markus H. Kainulainen
title	High-throughput quantitation of SARS-CoV-2 antibodies in a single-dilution homogeneous assay
title_short	High-throughput quantitation of SARS-CoV-2 antibodies in a single-dilution homogeneous assay
title_full	High-throughput quantitation of SARS-CoV-2 antibodies in a single-dilution homogeneous assay
title_fullStr	High-throughput quantitation of SARS-CoV-2 antibodies in a single-dilution homogeneous assay
title_full_unstemmed	High-throughput quantitation of SARS-CoV-2 antibodies in a single-dilution homogeneous assay
title_sort	high-throughput quantitation of sars-cov-2 antibodies in a single-dilution homogeneous assay
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/27dfd414a1384a84aa5cb8b2992de9c1
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High-throughput quantitation of SARS-CoV-2 antibodies in a single-dilution homogeneous assay

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