A UPLC-DAD-Based Bio-Screening Assay for the Evaluation of the Angiotensin Converting Enzyme Inhibitory Potential of Plant Extracts and Compounds: Pyrroquinazoline Alkaloids from <i>Adhatoda vasica</i> as a Case Study
Angiotensin converting enzyme (ACE) plays a crucial role in regulating blood pressure in the human body. Identification of potential ACE inhibitors from medicinal plants supported the idea of repurposing these medicinal plants against hypertension. A method based on ultra-performance liquid chromato...
Guardado en:
| Autores principales: | , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/27fa4a08bf8840cab5e7af8a46b082a0 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:27fa4a08bf8840cab5e7af8a46b082a0 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:27fa4a08bf8840cab5e7af8a46b082a02021-11-25T18:28:51ZA UPLC-DAD-Based Bio-Screening Assay for the Evaluation of the Angiotensin Converting Enzyme Inhibitory Potential of Plant Extracts and Compounds: Pyrroquinazoline Alkaloids from <i>Adhatoda vasica</i> as a Case Study10.3390/molecules262269711420-3049https://doaj.org/article/27fa4a08bf8840cab5e7af8a46b082a02021-11-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/22/6971https://doaj.org/toc/1420-3049Angiotensin converting enzyme (ACE) plays a crucial role in regulating blood pressure in the human body. Identification of potential ACE inhibitors from medicinal plants supported the idea of repurposing these medicinal plants against hypertension. A method based on ultra-performance liquid chromatography (UPLC) coupled with a diode array detector (DAD) was used for the rapid screening of plant extracts and purified compounds to determine their ACE inhibitory activity. Hippuryl-histidiyl-leucine (HHL) was used as a substrate, which is converted into hippuric acid (HA) by the action of ACE. A calibration curve of the substrate HHL was developed with the linear regression 0.999. The limits of detection and quantification of this method were found to be 0.134 and 0.4061 mM, respectively. Different parameters of ACE inhibitory assay were optimized, including concentration, incubation time and temperature. The ACE inhibition potential of <i>Adhatoda vasica</i> (methanolic-aqueous extract) and its isolated pyrroquinazoline alkaloids, vasicinol (<b>1</b>), vasicine (<b>2</b>) and vasicinone (<b>3</b>) was evaluated. Compounds <b>1</b>–<b>3</b> were characterized by various spectroscopic techniques. The IC<sub>50</sub> values of vasicinol (<b>1</b>), vasicine (<b>2</b>) and vasicinone (<b>3</b>) were found to be 6.45, 2.60 and 13.49 mM, respectively. Molecular docking studies of compounds <b>1</b>–<b>3</b> were also performed. Among these compounds, vasicinol (<b>1</b>) binds as effectively as captopril, a standard drug of ACE inhibition.Syeda TehreemSaeedur RahmanMuhammad Salman BhattiReaz UddinMuhammad Noman KhanSaba TauseefHesham R. El-SeediAbdullatif Bin MuhsinahJalal UddinSyed Ghulam MusharrafMDPI AGarticleangiotensin converting enzymehypertensionpyrroquinazoline alkaloidsvasicinevasicinolvasicinoneOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6971, p 6971 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
angiotensin converting enzyme hypertension pyrroquinazoline alkaloids vasicine vasicinol vasicinone Organic chemistry QD241-441 |
| spellingShingle |
angiotensin converting enzyme hypertension pyrroquinazoline alkaloids vasicine vasicinol vasicinone Organic chemistry QD241-441 Syeda Tehreem Saeedur Rahman Muhammad Salman Bhatti Reaz Uddin Muhammad Noman Khan Saba Tauseef Hesham R. El-Seedi Abdullatif Bin Muhsinah Jalal Uddin Syed Ghulam Musharraf A UPLC-DAD-Based Bio-Screening Assay for the Evaluation of the Angiotensin Converting Enzyme Inhibitory Potential of Plant Extracts and Compounds: Pyrroquinazoline Alkaloids from <i>Adhatoda vasica</i> as a Case Study |
| description |
Angiotensin converting enzyme (ACE) plays a crucial role in regulating blood pressure in the human body. Identification of potential ACE inhibitors from medicinal plants supported the idea of repurposing these medicinal plants against hypertension. A method based on ultra-performance liquid chromatography (UPLC) coupled with a diode array detector (DAD) was used for the rapid screening of plant extracts and purified compounds to determine their ACE inhibitory activity. Hippuryl-histidiyl-leucine (HHL) was used as a substrate, which is converted into hippuric acid (HA) by the action of ACE. A calibration curve of the substrate HHL was developed with the linear regression 0.999. The limits of detection and quantification of this method were found to be 0.134 and 0.4061 mM, respectively. Different parameters of ACE inhibitory assay were optimized, including concentration, incubation time and temperature. The ACE inhibition potential of <i>Adhatoda vasica</i> (methanolic-aqueous extract) and its isolated pyrroquinazoline alkaloids, vasicinol (<b>1</b>), vasicine (<b>2</b>) and vasicinone (<b>3</b>) was evaluated. Compounds <b>1</b>–<b>3</b> were characterized by various spectroscopic techniques. The IC<sub>50</sub> values of vasicinol (<b>1</b>), vasicine (<b>2</b>) and vasicinone (<b>3</b>) were found to be 6.45, 2.60 and 13.49 mM, respectively. Molecular docking studies of compounds <b>1</b>–<b>3</b> were also performed. Among these compounds, vasicinol (<b>1</b>) binds as effectively as captopril, a standard drug of ACE inhibition. |
| format |
article |
| author |
Syeda Tehreem Saeedur Rahman Muhammad Salman Bhatti Reaz Uddin Muhammad Noman Khan Saba Tauseef Hesham R. El-Seedi Abdullatif Bin Muhsinah Jalal Uddin Syed Ghulam Musharraf |
| author_facet |
Syeda Tehreem Saeedur Rahman Muhammad Salman Bhatti Reaz Uddin Muhammad Noman Khan Saba Tauseef Hesham R. El-Seedi Abdullatif Bin Muhsinah Jalal Uddin Syed Ghulam Musharraf |
| author_sort |
Syeda Tehreem |
| title |
A UPLC-DAD-Based Bio-Screening Assay for the Evaluation of the Angiotensin Converting Enzyme Inhibitory Potential of Plant Extracts and Compounds: Pyrroquinazoline Alkaloids from <i>Adhatoda vasica</i> as a Case Study |
| title_short |
A UPLC-DAD-Based Bio-Screening Assay for the Evaluation of the Angiotensin Converting Enzyme Inhibitory Potential of Plant Extracts and Compounds: Pyrroquinazoline Alkaloids from <i>Adhatoda vasica</i> as a Case Study |
| title_full |
A UPLC-DAD-Based Bio-Screening Assay for the Evaluation of the Angiotensin Converting Enzyme Inhibitory Potential of Plant Extracts and Compounds: Pyrroquinazoline Alkaloids from <i>Adhatoda vasica</i> as a Case Study |
| title_fullStr |
A UPLC-DAD-Based Bio-Screening Assay for the Evaluation of the Angiotensin Converting Enzyme Inhibitory Potential of Plant Extracts and Compounds: Pyrroquinazoline Alkaloids from <i>Adhatoda vasica</i> as a Case Study |
| title_full_unstemmed |
A UPLC-DAD-Based Bio-Screening Assay for the Evaluation of the Angiotensin Converting Enzyme Inhibitory Potential of Plant Extracts and Compounds: Pyrroquinazoline Alkaloids from <i>Adhatoda vasica</i> as a Case Study |
| title_sort |
uplc-dad-based bio-screening assay for the evaluation of the angiotensin converting enzyme inhibitory potential of plant extracts and compounds: pyrroquinazoline alkaloids from <i>adhatoda vasica</i> as a case study |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/27fa4a08bf8840cab5e7af8a46b082a0 |
| work_keys_str_mv |
AT syedatehreem auplcdadbasedbioscreeningassayfortheevaluationoftheangiotensinconvertingenzymeinhibitorypotentialofplantextractsandcompoundspyrroquinazolinealkaloidsfromiadhatodavasicaiasacasestudy AT saeedurrahman auplcdadbasedbioscreeningassayfortheevaluationoftheangiotensinconvertingenzymeinhibitorypotentialofplantextractsandcompoundspyrroquinazolinealkaloidsfromiadhatodavasicaiasacasestudy AT muhammadsalmanbhatti auplcdadbasedbioscreeningassayfortheevaluationoftheangiotensinconvertingenzymeinhibitorypotentialofplantextractsandcompoundspyrroquinazolinealkaloidsfromiadhatodavasicaiasacasestudy AT reazuddin auplcdadbasedbioscreeningassayfortheevaluationoftheangiotensinconvertingenzymeinhibitorypotentialofplantextractsandcompoundspyrroquinazolinealkaloidsfromiadhatodavasicaiasacasestudy AT muhammadnomankhan auplcdadbasedbioscreeningassayfortheevaluationoftheangiotensinconvertingenzymeinhibitorypotentialofplantextractsandcompoundspyrroquinazolinealkaloidsfromiadhatodavasicaiasacasestudy AT sabatauseef auplcdadbasedbioscreeningassayfortheevaluationoftheangiotensinconvertingenzymeinhibitorypotentialofplantextractsandcompoundspyrroquinazolinealkaloidsfromiadhatodavasicaiasacasestudy AT heshamrelseedi auplcdadbasedbioscreeningassayfortheevaluationoftheangiotensinconvertingenzymeinhibitorypotentialofplantextractsandcompoundspyrroquinazolinealkaloidsfromiadhatodavasicaiasacasestudy AT abdullatifbinmuhsinah auplcdadbasedbioscreeningassayfortheevaluationoftheangiotensinconvertingenzymeinhibitorypotentialofplantextractsandcompoundspyrroquinazolinealkaloidsfromiadhatodavasicaiasacasestudy AT jalaluddin auplcdadbasedbioscreeningassayfortheevaluationoftheangiotensinconvertingenzymeinhibitorypotentialofplantextractsandcompoundspyrroquinazolinealkaloidsfromiadhatodavasicaiasacasestudy AT syedghulammusharraf auplcdadbasedbioscreeningassayfortheevaluationoftheangiotensinconvertingenzymeinhibitorypotentialofplantextractsandcompoundspyrroquinazolinealkaloidsfromiadhatodavasicaiasacasestudy AT syedatehreem uplcdadbasedbioscreeningassayfortheevaluationoftheangiotensinconvertingenzymeinhibitorypotentialofplantextractsandcompoundspyrroquinazolinealkaloidsfromiadhatodavasicaiasacasestudy AT saeedurrahman uplcdadbasedbioscreeningassayfortheevaluationoftheangiotensinconvertingenzymeinhibitorypotentialofplantextractsandcompoundspyrroquinazolinealkaloidsfromiadhatodavasicaiasacasestudy AT muhammadsalmanbhatti uplcdadbasedbioscreeningassayfortheevaluationoftheangiotensinconvertingenzymeinhibitorypotentialofplantextractsandcompoundspyrroquinazolinealkaloidsfromiadhatodavasicaiasacasestudy AT reazuddin uplcdadbasedbioscreeningassayfortheevaluationoftheangiotensinconvertingenzymeinhibitorypotentialofplantextractsandcompoundspyrroquinazolinealkaloidsfromiadhatodavasicaiasacasestudy AT muhammadnomankhan uplcdadbasedbioscreeningassayfortheevaluationoftheangiotensinconvertingenzymeinhibitorypotentialofplantextractsandcompoundspyrroquinazolinealkaloidsfromiadhatodavasicaiasacasestudy AT sabatauseef uplcdadbasedbioscreeningassayfortheevaluationoftheangiotensinconvertingenzymeinhibitorypotentialofplantextractsandcompoundspyrroquinazolinealkaloidsfromiadhatodavasicaiasacasestudy AT heshamrelseedi uplcdadbasedbioscreeningassayfortheevaluationoftheangiotensinconvertingenzymeinhibitorypotentialofplantextractsandcompoundspyrroquinazolinealkaloidsfromiadhatodavasicaiasacasestudy AT abdullatifbinmuhsinah uplcdadbasedbioscreeningassayfortheevaluationoftheangiotensinconvertingenzymeinhibitorypotentialofplantextractsandcompoundspyrroquinazolinealkaloidsfromiadhatodavasicaiasacasestudy AT jalaluddin uplcdadbasedbioscreeningassayfortheevaluationoftheangiotensinconvertingenzymeinhibitorypotentialofplantextractsandcompoundspyrroquinazolinealkaloidsfromiadhatodavasicaiasacasestudy AT syedghulammusharraf uplcdadbasedbioscreeningassayfortheevaluationoftheangiotensinconvertingenzymeinhibitorypotentialofplantextractsandcompoundspyrroquinazolinealkaloidsfromiadhatodavasicaiasacasestudy |
| _version_ |
1718411064115200000 |