Effects of inflammatory and anti-inflammatory environments on the macrophage mitochondrial function

Abstract Mitochondrial response to inflammation is crucial in the metabolic adaptation to infection. This study aimed to explore the mitochondrial response under inflammatory and anti-inflammatory environments, with a focus on the tricarboxylic acid (TCA) cycle. Expression levels of key TCA cycle en...

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Autores principales:	Dong Ji, Jian-yun Yin, Dan-feng Li, Chang-tai Zhu, Jian-ping Ye, Yuan-qing Pan
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/27fd3661c2754e128fbfb31ffa690e6b
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spelling	oai:doaj.org-article:27fd3661c2754e128fbfb31ffa690e6b2021-12-02T11:42:10ZEffects of inflammatory and anti-inflammatory environments on the macrophage mitochondrial function10.1038/s41598-020-77370-x2045-2322https://doaj.org/article/27fd3661c2754e128fbfb31ffa690e6b2020-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-77370-xhttps://doaj.org/toc/2045-2322Abstract Mitochondrial response to inflammation is crucial in the metabolic adaptation to infection. This study aimed to explore the mitochondrial response under inflammatory and anti-inflammatory environments, with a focus on the tricarboxylic acid (TCA) cycle. Expression levels of key TCA cycle enzymes and the autophagy-related protein light chain 3b (LC3b) were determined in raw 264.7 cells treated with lipopolysaccharide (LPS) and metformin (Met). Additionally, reactive oxygen species (ROS) levels and mitochondrial membrane potential were assessed using flow cytometry. Moreover, 8-week-old C57BL/6J mice were intraperitoneally injected with LPS and Met to assess the mitochondrial response in vivo. Upon LPS stimulation, the expression of key TCA enzymes, including citrate synthase, α-ketoglutarate dehydrogenase, and isocitrate dehydrogenase 2, and the mitochondrial membrane potential decreased, whereas the levels of LC3b and ROS increased. However, treatment with Met inhibited the reduction of LPS-induced enzyme levels as well as the elevation of LC3b and ROS levels. In conclusion, the mitochondrial TCA cycle is affected by the inflammatory environment, and the LPS-induced effects can be reversed by Met treatment.Dong JiJian-yun YinDan-feng LiChang-tai ZhuJian-ping YeYuan-qing PanNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Dong Ji Jian-yun Yin Dan-feng Li Chang-tai Zhu Jian-ping Ye Yuan-qing Pan Effects of inflammatory and anti-inflammatory environments on the macrophage mitochondrial function
description	Abstract Mitochondrial response to inflammation is crucial in the metabolic adaptation to infection. This study aimed to explore the mitochondrial response under inflammatory and anti-inflammatory environments, with a focus on the tricarboxylic acid (TCA) cycle. Expression levels of key TCA cycle enzymes and the autophagy-related protein light chain 3b (LC3b) were determined in raw 264.7 cells treated with lipopolysaccharide (LPS) and metformin (Met). Additionally, reactive oxygen species (ROS) levels and mitochondrial membrane potential were assessed using flow cytometry. Moreover, 8-week-old C57BL/6J mice were intraperitoneally injected with LPS and Met to assess the mitochondrial response in vivo. Upon LPS stimulation, the expression of key TCA enzymes, including citrate synthase, α-ketoglutarate dehydrogenase, and isocitrate dehydrogenase 2, and the mitochondrial membrane potential decreased, whereas the levels of LC3b and ROS increased. However, treatment with Met inhibited the reduction of LPS-induced enzyme levels as well as the elevation of LC3b and ROS levels. In conclusion, the mitochondrial TCA cycle is affected by the inflammatory environment, and the LPS-induced effects can be reversed by Met treatment.
format	article
author	Dong Ji Jian-yun Yin Dan-feng Li Chang-tai Zhu Jian-ping Ye Yuan-qing Pan
author_facet	Dong Ji Jian-yun Yin Dan-feng Li Chang-tai Zhu Jian-ping Ye Yuan-qing Pan
author_sort	Dong Ji
title	Effects of inflammatory and anti-inflammatory environments on the macrophage mitochondrial function
title_short	Effects of inflammatory and anti-inflammatory environments on the macrophage mitochondrial function
title_full	Effects of inflammatory and anti-inflammatory environments on the macrophage mitochondrial function
title_fullStr	Effects of inflammatory and anti-inflammatory environments on the macrophage mitochondrial function
title_full_unstemmed	Effects of inflammatory and anti-inflammatory environments on the macrophage mitochondrial function
title_sort	effects of inflammatory and anti-inflammatory environments on the macrophage mitochondrial function
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/27fd3661c2754e128fbfb31ffa690e6b
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Effects of inflammatory and anti-inflammatory environments on the macrophage mitochondrial function

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