Characterization of normal and cancer stem-like cell populations in murine lingual epithelial organoids using single-cell RNA sequencing

Abstract The advances in oral cancer research and therapies have not improved the prognosis of patients with tongue cancer. The poor treatment response of tongue cancer may be attributed to the presence of heterogeneous tumor cells exhibiting stem cell characteristics. Therefore, there is a need to...

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Autores principales:	Erik Johansson, Hiroo Ueno
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/2800cd5ec03e4a009571454fc8bc0faf
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spelling	oai:doaj.org-article:2800cd5ec03e4a009571454fc8bc0faf2021-11-21T12:23:05ZCharacterization of normal and cancer stem-like cell populations in murine lingual epithelial organoids using single-cell RNA sequencing10.1038/s41598-021-01783-52045-2322https://doaj.org/article/2800cd5ec03e4a009571454fc8bc0faf2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01783-5https://doaj.org/toc/2045-2322Abstract The advances in oral cancer research and therapies have not improved the prognosis of patients with tongue cancer. The poor treatment response of tongue cancer may be attributed to the presence of heterogeneous tumor cells exhibiting stem cell characteristics. Therefore, there is a need to develop effective molecular-targeted therapies based on the specific gene expression profiles of these cancer stem-like cell populations. In this study, the characteristics of normal and cancerous organoids, which are convenient tools for screening anti-cancer drugs, were analyzed comparatively. As organoids are generally generated by single progenitors, they enable the exclusion of normal cell contamination from the analyses. Single-cell RNA sequencing analysis revealed that p53 signaling activation and negative regulation of cell cycle were enriched characteristics in normal stem-like cells whereas hypoxia-related pathways, such as HIF-1 signaling and glycolysis, were upregulated in cancer stem-like cells. The findings of this study improved our understanding of the common features of heterogeneous cell populations with stem cell properties in tongue cancers, that are different from those of normal stem cell populations; this will enable the development of novel molecular-targeted therapies for tongue cancer.Erik JohanssonHiroo UenoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Erik Johansson Hiroo Ueno Characterization of normal and cancer stem-like cell populations in murine lingual epithelial organoids using single-cell RNA sequencing
description	Abstract The advances in oral cancer research and therapies have not improved the prognosis of patients with tongue cancer. The poor treatment response of tongue cancer may be attributed to the presence of heterogeneous tumor cells exhibiting stem cell characteristics. Therefore, there is a need to develop effective molecular-targeted therapies based on the specific gene expression profiles of these cancer stem-like cell populations. In this study, the characteristics of normal and cancerous organoids, which are convenient tools for screening anti-cancer drugs, were analyzed comparatively. As organoids are generally generated by single progenitors, they enable the exclusion of normal cell contamination from the analyses. Single-cell RNA sequencing analysis revealed that p53 signaling activation and negative regulation of cell cycle were enriched characteristics in normal stem-like cells whereas hypoxia-related pathways, such as HIF-1 signaling and glycolysis, were upregulated in cancer stem-like cells. The findings of this study improved our understanding of the common features of heterogeneous cell populations with stem cell properties in tongue cancers, that are different from those of normal stem cell populations; this will enable the development of novel molecular-targeted therapies for tongue cancer.
format	article
author	Erik Johansson Hiroo Ueno
author_facet	Erik Johansson Hiroo Ueno
author_sort	Erik Johansson
title	Characterization of normal and cancer stem-like cell populations in murine lingual epithelial organoids using single-cell RNA sequencing
title_short	Characterization of normal and cancer stem-like cell populations in murine lingual epithelial organoids using single-cell RNA sequencing
title_full	Characterization of normal and cancer stem-like cell populations in murine lingual epithelial organoids using single-cell RNA sequencing
title_fullStr	Characterization of normal and cancer stem-like cell populations in murine lingual epithelial organoids using single-cell RNA sequencing
title_full_unstemmed	Characterization of normal and cancer stem-like cell populations in murine lingual epithelial organoids using single-cell RNA sequencing
title_sort	characterization of normal and cancer stem-like cell populations in murine lingual epithelial organoids using single-cell rna sequencing
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/2800cd5ec03e4a009571454fc8bc0faf
work_keys_str_mv	AT erikjohansson characterizationofnormalandcancerstemlikecellpopulationsinmurinelingualepithelialorganoidsusingsinglecellrnasequencing AT hirooueno characterizationofnormalandcancerstemlikecellpopulationsinmurinelingualepithelialorganoidsusingsinglecellrnasequencing
_version_	1718419053309067264

Characterization of normal and cancer stem-like cell populations in murine lingual epithelial organoids using single-cell RNA sequencing

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